FastQCFastQC Report
Sun 2 Apr 2023
EGAF00000785485

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000785485
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40171400
Sequences flagged as poor quality0
Sequence length42
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGAT402199910.012095670053819TruSeq Adapter, Index 5 (100% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATC1110760.2765051753237378TruSeq Adapter, Index 5 (100% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGCGAT404570.10071095356397836TruSeq Adapter, Index 5 (97% over 41bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)