FastQCFastQC Report
Wed 14 Sep 2022
EGAF00000785486

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000785486
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35417430
Sequences flagged as poor quality0
Sequence length42
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTAT932152426.319029923966813TruSeq Adapter, Index 2 (100% over 41bp)
ATATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTAT783100.2211058227545025TruSeq Adapter, Index 2 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCCGATGTATC696280.19659246873643854TruSeq Adapter, Index 9 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATC614430.17348237859155788TruSeq Adapter, Index 2 (100% over 42bp)
AGTTCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTAT582580.1644896312352421TruSeq Adapter, Index 2 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGAAT369260.10425939996210906TruSeq Adapter, Index 2 (97% over 41bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)