FastQCFastQC Report
Sun 2 Apr 2023
EGAF00000785489

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000785489
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51355234
Sequences flagged as poor quality0
Sequence length42
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAAT593818211.562953836409353TruSeq Adapter, Index 12 (100% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCCGATGTATC898090.17487798809367708TruSeq Adapter, Index 9 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTAT665890.12966351199957535TruSeq Adapter, Index 2 (100% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATC572800.1115368299168883TruSeq Adapter, Index 12 (100% over 42bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)