@HD VN:1.4 SO:coordinate @RG ID:1#215 PL:ILLUMINA PU:141128_MS5_14817_A_MS2786524-500V2_1#215 LB:12425779 DS:EGAS00001000814: The aim of this study is to ascertain whether leukaemic mutations exist within the blood of people with otherwise normal haematopoeisis. To satisfy this aim we plan to look for 7 known leukaemic mutations in the whole blood DNA of a large cohort of blood donors who have normal haematopoesis. Genomic regions around mutational sites have been amplified using a 2 step PCR process which involves barcoding of individual patients DT:2014-11-28T00:00:00+0000 SM:UKHLS_983 PG:BamIndexDecoder CN:SC @PG ID:SCS PN:MiSeq Control Software DS:Controlling software on instrument VN:2.5.0.5 @PG ID:basecalling PN:RTA PP:SCS DS:Basecalling Package VN:1.18.54.0 @PG ID:Illumina2bam PN:Illumina2bam PP:basecalling DS:Convert Illumina BCL to BAM or SAM file VN:V1.16 CL:uk.ac.sanger.npg.illumina.Illumina2bam INTENSITY_DIR=/nfs/sf49/ILorHSorMS_sf49/analysis/141128_MS5_14817_A_MS2786524-500V2/Data/Intensities BASECALLS_DIR=/nfs/sf49/ILorHSorMS_sf49/analysis/141128_MS5_14817_A_MS2786524-500V2/Data/Intensities/BaseCalls LANE=1 OUTPUT=/dev/stdout SAMPLE_ALIAS=UKHLS_769,UKHLS_770,UKHLS_771,UKHLS_772,UKHLS_773,UKHLS_774,UKHLS_775,UKHLS_776,UKHLS_777,UKHLS_778,UKHLS_779,UKHLS_780,UKHLS_781,UKHLS_782,UKHLS_783,UKHLS_784,UKHLS_785,UKHLS_786,UKHLS_787,UKHLS_788,UKHLS_789,UKHLS_790,UKHLS_791,UKHLS_792,UKHLS_793,UKHLS_794,UKHLS_795,UKHLS_796,UKHLS_797,UKHLS_798,UKHLS_799,UKHLS_800,UKHLS_801,UKHLS_802,UKHLS_803,UKHLS_804,UKHLS_805,UKHLS_806,UKHLS_807,UKHLS_808,UKHLS_809,UKHLS_810,UKHLS_811,UKHLS_812,UKHLS_813,UKHLS_814,UKHLS_815,UKHLS_816,UKHLS_817,UKHLS_818,UKHLS_819,UKHLS_820,UKHLS_821,UKHLS_822,UKHLS_823,UKHLS_824,UKHLS_825,UKHLS_826,UKHLS_827,UKHLS_828,UKHLS_829,UKHLS_830,UKHLS_831,UKHLS_832,UKHLS_833,UKHLS_834,UKHLS_835,UKHLS_836,UKHLS_837,UKHLS_838,UKHLS_839,UKHLS_840,UKHLS_841,UKHLS_842,UKHLS_843,UKHLS_844,UKHLS_845,UKHLS_846,UKHLS_847,UKHLS_848,UKHLS_849,UKHLS_850,UKHLS_851,UKHLS_852,UKHLS_853,UKHLS_854,UKHLS_855,UKHLS_856,UKHLS_857,UKHLS_858,UKHLS_859,UKHLS_860,UKHLS_861,UKHLS_862,UKHLS_863,UKHLS_864,UKHLS_865,UKHLS_866,UKHLS_867,UKHLS_868,UKHLS_869,UKHLS_870,UKHLS_871,UKHLS_872,UKHLS_873,UKHLS_874,UKHLS_875,UKHLS_876,UKHLS_877,UKHLS_878,UKHLS_879,UKHLS_880,UKHLS_881,UKHLS_882,UKHLS_883,UKHLS_884,UKHLS_885,UKHLS_886,UKHLS_887,UKHLS_888,UKHLS_889,UKHLS_890,UKHLS_891,UKHLS_892,UKHLS_893,UKHLS_894,UKHLS_895,UKHLS_896,UKHLS_897,UKHLS_898,UKHLS_899,UKHLS_900,UKHLS_901,UKHLS_902,UKHLS_903,UKHLS_904,UKHLS_905,UKHLS_906,UKHLS_907,UKHLS_908,UKHLS_909,UKHLS_910,UKHLS_911,UKHLS_912,UKHLS_913,UKHLS_914,UKHLS_915,UKHLS_916,UKHLS_917,UKHLS_918,UKHLS_919,UKHLS_920,UKHLS_921,UKHLS_922,UKHLS_923,UKHLS_924,UKHLS_925,UKHLS_926,UKHLS_927,UKHLS_928,UKHLS_929,UKHLS_930,UKHLS_931,UKHLS_932,UKHLS_933,UKHLS_934,UKHLS_935,UKHLS_936,UKHLS_937,UKHLS_938,UKHLS_939,UKHLS_940,UKHLS_941,UKHLS_942,UKHLS_943,UKHLS_944,UKHLS_945,UKHLS_946,UKHLS_947,UKHLS_948,UKHLS_949,UKHLS_950,UKHLS_951,UKHLS_952,UKHLS_953,UKHLS_954,UKHLS_955,UKHLS_956,UKHLS_957,UKHLS_958,UKHLS_959,UKHLS_960,UKHLS_961,UKHLS_962,UKHLS_963,UKHLS_964,UKHLS_965,UKHLS_966,UKHLS_967,UKHLS_968,UKHLS_969,UKHLS_970,UKHLS_971,UKHLS_972,UKHLS_973,UKHLS_974,UKHLS_975,UKHLS_976,UKHLS_977,UKHLS_978,UKHLS_979,UKHLS_980,UKHLS_981,UKHLS_982,UKHLS_983,UKHLS_984,UKHLS_985,UKHLS_986,UKHLS_987,UKHLS_988,UKHLS_989,UKHLS_990,UKHLS_991,UKHLS_992,UKHLS_993,UKHLS_994,UKHLS_995,UKHLS_996,UKHLS_997,UKHLS_998,UKHLS_999,UKHLS_1000,UKHLS_1001,UKHLS_1002,UKHLS_1003,UKHLS_1004,UKHLS_1005,UKHLS_1006,UKHLS_1007,UKHLS_1008,UKHLS_1009,UKHLS_1010,UKHLS_1011,UKHLS_1012,UKHLS_1013,UKHLS_1014,UKHLS_1015,UKHLS_1016,UKHLS_1017,UKHLS_1018,UKHLS_1019,UKHLS_1020,UKHLS_1021,UKHLS_1022,UKHLS_1023,UKHLS_1024,UKHLS_1025,UKHLS_1026,UKHLS_1027,UKHLS_1028,UKHLS_1029,UKHLS_1030,UKHLS_1031,UKHLS_1032,UKHLS_1033,UKHLS_1034,UKHLS_1035,UKHLS_1036,UKHLS_1037,UKHLS_1038,UKHLS_1039,UKHLS_1040,UKHLS_1041,UKHLS_1042,UKHLS_1043,UKHLS_1044,UKHLS_1045,UKHLS_1046,UKHLS_1047,UKHLS_1048,UKHLS_1049,UKHLS_1050,UKHLS_1051,UKHLS_1052,UKHLS_1053,UKHLS_1054,UKHLS_1055,UKHLS_1056,UKHLS_1057,UKHLS_1058,UKHLS_1059,UKHLS_1060,UKHLS_1061,UKHLS_1062,UKHLS_1063,UKHLS_1064,UKHLS_1065,UKHLS_1066,UKHLS_1067,UKHLS_1068,UKHLS_1069,UKHLS_1070,UKHLS_1071,UKHLS_1072,UKHLS_1073,UKHLS_1074,UKHLS_1075,UKHLS_1076,UKHLS_1077,UKHLS_1078,UKHLS_1079,UKHLS_1080,UKHLS_1081,UKHLS_1082,UKHLS_1083,UKHLS_1084,UKHLS_1085,UKHLS_1086,UKHLS_1087,UKHLS_1088,UKHLS_1089,UKHLS_1090,UKHLS_1091,UKHLS_1092,UKHLS_1093,UKHLS_1094,UKHLS_1095,UKHLS_1096,UKHLS_1097,UKHLS_1098,UKHLS_1099,UKHLS_1100,UKHLS_1101,UKHLS_1102,UKHLS_1103,UKHLS_1104,UKHLS_1105,UKHLS_1106,UKHLS_1107,UKHLS_1108,UKHLS_1109,UKHLS_1110,UKHLS_1111,UKHLS_1112,UKHLS_1113,UKHLS_1114,UKHLS_1115,UKHLS_1116,UKHLS_1117,UKHLS_1118,UKHLS_1119,UKHLS_1120,UKHLS_1121,UKHLS_1122,UKHLS_1123,UKHLS_1124,UKHLS_1125,UKHLS_1126,UKHLS_1127,UKHLS_1128,UKHLS_1129,UKHLS_1130,UKHLS_1131,UKHLS_1132,UKHLS_1133,UKHLS_1134,UKHLS_1135,UKHLS_1136,UKHLS_1137,UKHLS_1138,UKHLS_1139,UKHLS_1140,UKHLS_1141,UKHLS_1142,UKHLS_1143,UKHLS_1144,UKHLS_1145,UKHLS_1146,UKHLS_1147,UKHLS_1148,UKHLS_1149,UKHLS_1150,UKHLS_1151,UKHLS_1152 STUDY_NAME=EGAS00001000814: The aim of this study is to ascertain whether leukaemic mutations exist within the blood of people with otherwise normal haematopoeisis. To satisfy this aim we plan to look for 7 known leukaemic mutations in the whole blood DNA of a large cohort of blood donors who have normal haematopoesis. Genomic regions around mutational sites have been amplified using a 2 step PCR process which involves barcoding of individual patients PLATFORM_UNIT=141128_MS5_14817_A_MS2786524-500V2_1 COMPRESSION_LEVEL=0 GENERATE_SECONDARY_BASE_CALLS=false PF_FILTER=true READ_GROUP_ID=1 LIBRARY_NAME=unknown SEQUENCING_CENTER=SC PLATFORM=ILLUMINA BARCODE_SEQUENCE_TAG_NAME=BC BARCODE_QUALITY_TAG_NAME=QT VERBOSITY=INFO QUIET=false VALIDATION_STRINGENCY=STRICT MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false CREATE_MD5_FILE=false @PG ID:bamadapterfind PN:bamadapterfind PP:Illumina2bam VN:0.0.180 CL:bamadapterfind level=0 @PG ID:BamIndexDecoder PN:BamIndexDecoder PP:bamadapterfind DS:A command-line tool to decode multiplexed bam file VN:V1.16 CL:uk.ac.sanger.npg.picard.BamIndexDecoder INPUT=/dev/stdin OUTPUT=/dev/stdout BARCODE_FILE=/nfs/sf49/ILorHSorMS_sf49/analysis/141128_MS5_14817_A_MS2786524-500V2/Data/Intensities/BAM_basecalls_20141130-023102/metadata_cache_14817/lane_1.taglist METRICS_FILE=/nfs/sf49/ILorHSorMS_sf49/analysis/141128_MS5_14817_A_MS2786524-500V2/Data/Intensities/BAM_basecalls_20141130-023102/14817_1.bam.tag_decode.metrics VALIDATION_STRINGENCY=SILENT CREATE_MD5_FILE=false BARCODE_TAG_NAME=BC BARCODE_QUALITY_TAG_NAME=QT MAX_MISMATCHES=1 MIN_MISMATCH_DELTA=1 MAX_NO_CALLS=2 CONVERT_LOW_QUALITY_TO_NO_CALL=false MAX_LOW_QUALITY_TO_CONVERT=15 VERBOSITY=INFO QUIET=false COMPRESSION_LEVEL=5 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false @PG ID:SplitBamByReadGroup PN:SplitBamByReadGroup PP:BamIndexDecoder DS:Split a BAM file into multiple BAM files based on ReadGroup. Headers are a copy of the original file, removing @RGs where IDs match with the other ReadGroup IDs VN:V1.16 CL:uk.ac.sanger.npg.picard.SplitBamByReadGroup INPUT=/nfs/sf49/ILorHSorMS_sf49/analysis/141128_MS5_14817_A_MS2786524-500V2/Data/Intensities/BAM_basecalls_20141130-023102/no_cal/14817_1.bam OUTPUT_PREFIX=/nfs/sf49/ILorHSorMS_sf49/analysis/141128_MS5_14817_A_MS2786524-500V2/Data/Intensities/BAM_basecalls_20141130-023102/no_cal/lane1/14817_1 OUTPUT_COMMON_RG_HEAD_TO_TRIM=1 VALIDATION_STRINGENCY=SILENT CREATE_MD5_FILE=true VERBOSITY=INFO QUIET=false COMPRESSION_LEVEL=5 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false @PG ID:bwa_aln PN:bwa PP:SplitBamByReadGroup VN:0.5.10-tpx CL:/software/solexa/pkg/bwa/bwa-0.5.10-mt_fixes/bwa aln -q 15 -t 12 /lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/bwa/human_g1k_v37.fasta -b1 /nfs/sf49/ILorHSorMS_sf49/analysis/141128_MS5_14817_A_MS2786524-500V2/Data/Intensities/BAM_basecalls_20141130-023102/no_cal/lane1/14817_1#215.bam > /tmp/NdWcOC3L9R/1.sai @PG ID:bwa_aln_1 PN:bwa PP:bwa_aln VN:0.5.10-tpx CL:/software/solexa/pkg/bwa/bwa-0.5.10-mt_fixes/bwa aln -q 15 -t 12 /lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/bwa/human_g1k_v37.fasta -b2 /nfs/sf49/ILorHSorMS_sf49/analysis/141128_MS5_14817_A_MS2786524-500V2/Data/Intensities/BAM_basecalls_20141130-023102/no_cal/lane1/14817_1#215.bam > /tmp/NdWcOC3L9R/2.sai @PG ID:bwa_sam PN:bwa PP:bwa_aln_1 VN:0.5.10-tpx CL:/software/solexa/pkg/bwa/bwa-0.5.10-mt_fixes/bwa sampe -t 6 /lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/bwa/human_g1k_v37.fasta /tmp/NdWcOC3L9R/1.sai /tmp/NdWcOC3L9R/2.sai /nfs/sf49/ILorHSorMS_sf49/analysis/141128_MS5_14817_A_MS2786524-500V2/Data/Intensities/BAM_basecalls_20141130-023102/no_cal/lane1/14817_1#215.bam /nfs/sf49/ILorHSorMS_sf49/analysis/141128_MS5_14817_A_MS2786524-500V2/Data/Intensities/BAM_basecalls_20141130-023102/no_cal/lane1/14817_1#215.bam @PG ID:Picard_SamFormatConverter PN:SamFormatConverter PP:bwa_sam VN:1.72(1230) CL:/software/jdk1.7.0_25/bin/java -Xmx1000m -jar /software/solexa/pkg/picard/picard-tools-1.72/SamFormatConverter.jar VALIDATION_STRINGENCY=SILENT INPUT=/dev/stdin OUTPUT=/dev/stdout COMPRESSION_LEVEL=0 @PG ID:samtools_fixmate PN:samtools PP:Picard_SamFormatConverter VN:0.1.18 (r982:295) CL:/software/solexa/pkg/samtools/samtools-0.1.18/samtools fixmate - - @PG ID:BamMerger PN:BamMerger PP:samtools_fixmate DS:A command-line tool to merge BAM/SAM alignment info in the first input file with the data in an unmapped BAM file, producing a third BAM file that has alignment data and all the additional data from the unmapped BAM VN:V1.16 CL:uk.ac.sanger.npg.picard.BamMerger ALIGNED_BAM=/dev/stdin ALIGNMENT_PROGRAM_ID=NULL KEEP_ALL_PG=true INPUT=/nfs/sf49/ILorHSorMS_sf49/analysis/141128_MS5_14817_A_MS2786524-500V2/Data/Intensities/BAM_basecalls_20141130-023102/no_cal/lane1/14817_1#215.bam OUTPUT=/nfs/sf49/ILorHSorMS_sf49/analysis/141128_MS5_14817_A_MS2786524-500V2/Data/Intensities/BAM_basecalls_20141130-023102/no_cal/archive/lane1/14817_1#215.bam KEEP_EXTRA_UNMAPPED_READS=true VALIDATION_STRINGENCY=SILENT CREATE_MD5_FILE=true REPLACE_ALIGNED_BASE_QUALITY=false VERBOSITY=INFO QUIET=false COMPRESSION_LEVEL=5 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false @PG ID:bamsort PN:bamsort PP:BamMerger VN:0.0.180 CL:/software/hpag/biobambam/0.0.180/bin/bamsort tmpfile=/nfs/sf49/ILorHSorMS_sf49/analysis/141128_MS5_14817_A_MS2786524-500V2/Data/Intensities/BAM_basecalls_20141130-023102/no_cal/archive/lane1/iOXkHLLDZw/ @PG ID:bammarkduplicates2 PN:bammarkduplicates2 PP:bamsort VN:0.0.180 CL:/software/hpag/biobambam/0.0.180/bin/bammarkduplicates2 I=/nfs/sf49/ILorHSorMS_sf49/analysis/141128_MS5_14817_A_MS2786524-500V2/Data/Intensities/BAM_basecalls_20141130-023102/no_cal/archive/lane1/iOXkHLLDZw/sorted.bam O=/dev/stdout tmpfile=/nfs/sf49/ILorHSorMS_sf49/analysis/141128_MS5_14817_A_MS2786524-500V2/Data/Intensities/BAM_basecalls_20141130-023102/no_cal/archive/lane1/iOXkHLLDZw/ M=/tmp/40lE8M0gbR level=0 @PG ID:BamTagStripper PN:BamTagStripper PP:bammarkduplicates2 DS:Strip a list of tags in bam/sam record. By default, any tags containing lowercase letters will be stripped and other tags will be kept. A list of tags can be given to keep or strip VN:V1.16 CL:uk.ac.sanger.npg.picard.BamTagStripper INPUT=/dev/stdin OUTPUT=/dev/stdout TAG_TO_KEEP=[a3, aa, af, ah, as, br, qr, tq, tr] TAG_TO_STRIP=[OQ] TMP_DIR=[/nfs/sf49/ILorHSorMS_sf49/analysis/141128_MS5_14817_A_MS2786524-500V2/Data/Intensities/BAM_basecalls_20141130-023102/no_cal/archive/lane1/iOXkHLLDZw] VERBOSITY=INFO VALIDATION_STRINGENCY=SILENT CREATE_INDEX=false CREATE_MD5_FILE=false QUIET=false COMPRESSION_LEVEL=5 MAX_RECORDS_IN_RAM=500000 @PG ID:scramble PN:scramble PP:BamTagStripper VN:1.13.7 CL:/software/badger/bin/scramble -I bam -O cram -r /lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta @SQ SN:1 LN:249250621 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:1b22b98cdeb4a9304cb5d48026a85128 SP:Human @SQ SN:2 LN:243199373 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:a0d9851da00400dec1098a9255ac712e SP:Human @SQ SN:3 LN:198022430 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:fdfd811849cc2fadebc929bb925902e5 SP:Human @SQ SN:4 LN:191154276 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:23dccd106897542ad87d2765d28a19a1 SP:Human @SQ SN:5 LN:180915260 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:0740173db9ffd264d728f32784845cd7 SP:Human @SQ SN:6 LN:171115067 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:1d3a93a248d92a729ee764823acbbc6b SP:Human @SQ SN:7 LN:159138663 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:618366e953d6aaad97dbe4777c29375e SP:Human @SQ SN:8 LN:146364022 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:96f514a9929e410c6651697bded59aec SP:Human @SQ SN:9 LN:141213431 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:3e273117f15e0a400f01055d9f393768 SP:Human @SQ SN:10 LN:135534747 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:988c28e000e84c26d552359af1ea2e1d SP:Human @SQ SN:11 LN:135006516 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:98c59049a2df285c76ffb1c6db8f8b96 SP:Human @SQ SN:12 LN:133851895 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:51851ac0e1a115847ad36449b0015864 SP:Human @SQ SN:13 LN:115169878 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:283f8d7892baa81b510a015719ca7b0b SP:Human @SQ SN:14 LN:107349540 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:98f3cae32b2a2e9524bc19813927542e SP:Human @SQ SN:15 LN:102531392 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:e5645a794a8238215b2cd77acb95a078 SP:Human @SQ SN:16 LN:90354753 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:fc9b1a7b42b97a864f56b348b06095e6 SP:Human @SQ SN:17 LN:81195210 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:351f64d4f4f9ddd45b35336ad97aa6de SP:Human @SQ SN:18 LN:78077248 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:b15d4b2d29dde9d3e4f93d1d0f2cbc9c SP:Human @SQ SN:19 LN:59128983 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:1aacd71f30db8e561810913e0b72636d SP:Human @SQ SN:20 LN:63025520 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:0dec9660ec1efaaf33281c0d5ea2560f SP:Human @SQ SN:21 LN:48129895 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:2979a6085bfe28e3ad6f552f361ed74d SP:Human @SQ SN:22 LN:51304566 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:a718acaa6135fdca8357d5bfe94211dd SP:Human @SQ SN:X LN:155270560 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:7e0e2e580297b7764e31dbc80c2540dd SP:Human @SQ SN:Y LN:59373566 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:1fa3474750af0948bdf97d5a0ee52e51 SP:Human @SQ SN:MT LN:16569 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:c68f52674c9fb33aef52dcf399755519 SP:Human @SQ SN:GL000207.1 LN:4262 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:f3814841f1939d3ca19072d9e89f3fd7 SP:Human @SQ SN:GL000226.1 LN:15008 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:1c1b2cd1fccbc0a99b6a447fa24d1504 SP:Human @SQ SN:GL000229.1 LN:19913 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:d0f40ec87de311d8e715b52e4c7062e1 SP:Human @SQ SN:GL000231.1 LN:27386 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:ba8882ce3a1efa2080e5d29b956568a4 SP:Human @SQ SN:GL000210.1 LN:27682 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:851106a74238044126131ce2a8e5847c SP:Human @SQ SN:GL000239.1 LN:33824 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:99795f15702caec4fa1c4e15f8a29c07 SP:Human @SQ SN:GL000235.1 LN:34474 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:118a25ca210cfbcdfb6c2ebb249f9680 SP:Human @SQ SN:GL000201.1 LN:36148 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:dfb7e7ec60ffdcb85cb359ea28454ee9 SP:Human @SQ SN:GL000247.1 LN:36422 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:7de00226bb7df1c57276ca6baabafd15 SP:Human @SQ SN:GL000245.1 LN:36651 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:89bc61960f37d94abf0df2d481ada0ec SP:Human @SQ SN:GL000197.1 LN:37175 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:6f5efdd36643a9b8c8ccad6f2f1edc7b SP:Human @SQ SN:GL000203.1 LN:37498 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:96358c325fe0e70bee73436e8bb14dbd SP:Human @SQ SN:GL000246.1 LN:38154 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:e4afcd31912af9d9c2546acf1cb23af2 SP:Human @SQ SN:GL000249.1 LN:38502 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:1d78abec37c15fe29a275eb08d5af236 SP:Human @SQ SN:GL000196.1 LN:38914 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:d92206d1bb4c3b4019c43c0875c06dc0 SP:Human @SQ SN:GL000248.1 LN:39786 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:5a8e43bec9be36c7b49c84d585107776 SP:Human @SQ SN:GL000244.1 LN:39929 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:0996b4475f353ca98bacb756ac479140 SP:Human @SQ SN:GL000238.1 LN:39939 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:131b1efc3270cc838686b54e7c34b17b SP:Human @SQ SN:GL000202.1 LN:40103 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:06cbf126247d89664a4faebad130fe9c SP:Human @SQ SN:GL000234.1 LN:40531 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:93f998536b61a56fd0ff47322a911d4b SP:Human @SQ SN:GL000232.1 LN:40652 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:3e06b6741061ad93a8587531307057d8 SP:Human @SQ SN:GL000206.1 LN:41001 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:43f69e423533e948bfae5ce1d45bd3f1 SP:Human @SQ SN:GL000240.1 LN:41933 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:445a86173da9f237d7bcf41c6cb8cc62 SP:Human @SQ SN:GL000236.1 LN:41934 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:fdcd739913efa1fdc64b6c0cd7016779 SP:Human @SQ SN:GL000241.1 LN:42152 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:ef4258cdc5a45c206cea8fc3e1d858cf SP:Human @SQ SN:GL000243.1 LN:43341 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:cc34279a7e353136741c9fce79bc4396 SP:Human @SQ SN:GL000242.1 LN:43523 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:2f8694fc47576bc81b5fe9e7de0ba49e SP:Human @SQ SN:GL000230.1 LN:43691 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:b4eb71ee878d3706246b7c1dbef69299 SP:Human @SQ SN:GL000237.1 LN:45867 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:e0c82e7751df73f4f6d0ed30cdc853c0 SP:Human @SQ SN:GL000233.1 LN:45941 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:7fed60298a8d62ff808b74b6ce820001 SP:Human @SQ SN:GL000204.1 LN:81310 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:efc49c871536fa8d79cb0a06fa739722 SP:Human @SQ SN:GL000198.1 LN:90085 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:868e7784040da90d900d2d1b667a1383 SP:Human @SQ SN:GL000208.1 LN:92689 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:aa81be49bf3fe63a79bdc6a6f279abf6 SP:Human @SQ SN:GL000191.1 LN:106433 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:d75b436f50a8214ee9c2a51d30b2c2cc SP:Human @SQ SN:GL000227.1 LN:128374 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:a4aead23f8053f2655e468bcc6ecdceb SP:Human @SQ SN:GL000228.1 LN:129120 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:c5a17c97e2c1a0b6a9cc5a6b064b714f SP:Human @SQ SN:GL000214.1 LN:137718 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:46c2032c37f2ed899eb41c0473319a69 SP:Human @SQ SN:GL000221.1 LN:155397 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:3238fb74ea87ae857f9c7508d315babb SP:Human @SQ SN:GL000209.1 LN:159169 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:f40598e2a5a6b26e84a3775e0d1e2c81 SP:Human @SQ SN:GL000218.1 LN:161147 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:1d708b54644c26c7e01c2dad5426d38c SP:Human @SQ SN:GL000220.1 LN:161802 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:fc35de963c57bf7648429e6454f1c9db SP:Human @SQ SN:GL000213.1 LN:164239 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:9d424fdcc98866650b58f004080a992a SP:Human @SQ SN:GL000211.1 LN:166566 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:7daaa45c66b288847b9b32b964e623d3 SP:Human @SQ SN:GL000199.1 LN:169874 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:569af3b73522fab4b40995ae4944e78e SP:Human @SQ SN:GL000217.1 LN:172149 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:6d243e18dea1945fb7f2517615b8f52e SP:Human @SQ SN:GL000216.1 LN:172294 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:642a232d91c486ac339263820aef7fe0 SP:Human @SQ SN:GL000215.1 LN:172545 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:5eb3b418480ae67a997957c909375a73 SP:Human @SQ SN:GL000205.1 LN:174588 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:d22441398d99caf673e9afb9a1908ec5 SP:Human @SQ SN:GL000219.1 LN:179198 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:f977edd13bac459cb2ed4a5457dba1b3 SP:Human @SQ SN:GL000224.1 LN:179693 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:d5b2fc04f6b41b212a4198a07f450e20 SP:Human @SQ SN:GL000223.1 LN:180455 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:399dfa03bf32022ab52a846f7ca35b30 SP:Human @SQ SN:GL000195.1 LN:182896 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:5d9ec007868d517e73543b005ba48535 SP:Human @SQ SN:GL000212.1 LN:186858 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:563531689f3dbd691331fd6c5730a88b SP:Human @SQ SN:GL000222.1 LN:186861 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:6fe9abac455169f50470f5a6b01d0f59 SP:Human @SQ SN:GL000200.1 LN:187035 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:75e4c8d17cd4addf3917d1703cacaf25 SP:Human @SQ SN:GL000193.1 LN:189789 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:dbb6e8ece0b5de29da56601613007c2a SP:Human @SQ SN:GL000194.1 LN:191469 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:6ac8f815bf8e845bb3031b73f812c012 SP:Human @SQ SN:GL000225.1 LN:211173 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:63945c3e6962f28ffd469719a747e73c SP:Human @SQ SN:GL000192.1 LN:547496 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:325ba9e808f669dfeee210fdd7b470ac SP:Human