FastQCFastQC Report
Sat 23 Apr 2022
EGAF00000817470

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000817470
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Sequences flagged as poor quality0
Sequence length100
%GC26

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC207.0286286E-445.0087789
CGGAAGA451.9341314E-839.9912764
AAGAGCG403.455807E-739.382687
GAAGAGC403.455807E-739.382686
TCGGAAG356.2615436E-638.5630153
AGAGCGT250.002105714536.0070238
AGAGCGC250.002105714536.0070238
ATCGGAA250.00210998935.992152
GGAAGAG602.4176552E-730.0054745
AAATCGG250.002264861522.50157234-35
TGGGAAA250.002265850322.49988220-21
GTAGGGG250.00226634522.49903518-19
GGTAGGG250.002269644522.49340416-17
GGAAAGA458.261724E-619.99989322-23
TGGGGAA356.9886993E-419.28561220-21
GAAAGAG356.9886993E-419.28561222-23
GGGCCCC300.00649942818.75718142-43
GGGTAGG300.00652484718.74450316-17
AGGGAAA451.8675067E-417.49990720-21
GGGAAAA1005.0931703E-1116.87491222-23
Produced by FastQC (version 0.11.5)