FastQCFastQC Report
Sat 23 Apr 2022
EGAF00000817538

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000817538
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Sequences flagged as poor quality0
Sequence length100
%GC24

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGCG554.8747534E-628.06596444-45
GGGGGGC653.49919E-818.65923942-43
GGGAAAG300.00748386918.30424922-23
GGGAAAA400.00201089216.47382222-23
AAAGGGG450.004394757614.650084526-27
GGGGAAA755.3608295E-513.17905722-23
GGGGGAA1003.3479046E-713.17823420-21
GGGGGGA1150.00277365238.594520-21
Produced by FastQC (version 0.11.5)