FastQCFastQC Report
Sat 23 Apr 2022
EGAF00000817654

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000817654
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Sequences flagged as poor quality0
Sequence length100
%GC24

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAA404.138783E-738.487143
GAGCGGC357.297058E-637.712549
GAAAAGC357.297058E-637.712546
AGAGCGG357.297058E-637.712548
GGCGCCG601.9826984E-1036.67827244-45
CGGGAGA250.002353975335.1983684
AAAAGCG401.8216964E-532.9984667
AAGGGGC353.220056E-431.4271149
GGGGCCC300.005737696329.34261944-45
ATCGGAA1200.027.4932252
AAGAGCG701.1338216E-625.1416917
CGGAAAA801.3119825E-724.7488524
GAAGAGC651.8154E-523.691216
CGGAAGA651.8154E-523.691214
TCGGAAG752.0722637E-623.4588283
GATCGGA952.651177E-823.1890221
GGGCGCC605.984475E-1022.00696442-43
ATCTCGG555.2532414E-922.00448634-35
CTCGGGG504.622234E-822.00448636-37
TCTCGGG403.613357E-622.00448436-37
Produced by FastQC (version 0.11.5)