FastQCFastQC Report
Sat 23 Apr 2022
EGAF00000817666

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000817666
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Sequences flagged as poor quality0
Sequence length100
%GC24

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGCC402.9654002E-622.51136842-43
GGAAAAG352.6988442E-522.50727822-23
TGGGGAA302.4679696E-422.50178120-21
GGGAAAA1100.019.43810522-23
AGGGGAA300.006510833318.75148420-21
GGGGGAA552.2576914E-618.4105520-21
TCGGGGG400.001702985316.88839138-39
GGGGGGC609.7217744E-515.00757942-43
GGGGAAA951.3339013E-714.21165220-21
GGAAAAA1105.493166E-813.29975522-23
GGGGGGA600.0016627913.11987818-19
CGGGGGG650.003015049312.12029140-41
AAAAGGG750.00875395710.50497624-25
Produced by FastQC (version 0.11.5)