FastQCFastQC Report
Sat 23 Apr 2022
EGAF00000817818

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000817818
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Sequences flagged as poor quality0
Sequence length101
%GC26

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCCCG550.00258343534.537146
ATAGTAT300.003946963731.67548660-61
GGGGCCG605.404383E-627.69681744-45
GGGGGAA605.418062E-627.68781320-21
GTAGGGG350.008424384527.12309518-19
GAAGAGG350.00842592627.12207824-25
TTGGGGG458.928549E-426.40086438-39
GGGGAAA651.0091077E-525.55718422-23
GGGGGCC500.001650201323.7606342-43
GGGGGGC704.8405898E-420.36625342-43
AAAGGGG650.00759936318.25570126-27
GTGGTCG1501.4691941E-615.83279194-95
ATATAGA1000.005260518314.25860990-91
CGGTGGT1809.707273E-613.19671692-93
CTCAGAT1607.039869E-411.87704492-93
TATTAGA1450.004560868711.46749192-93
TCTAGAT1500.00585290111.09002990-91
CTCGGTG2259.467345E-510.56193290-91
CTAGATC4056.712071E-1010.56193290-91
TCGGTGG2054.7420326E-410.42647294-95
Produced by FastQC (version 0.11.5)