FastQCFastQC Report
Sat 23 Apr 2022
EGAF00000818177

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000818177
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Sequences flagged as poor quality0
Sequence length100
%GC27

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCTTGA5100.043.926360-61
GCTTGAA4950.043.8282260-61
CTGCTTG4900.043.79419358-59
CTTGAAA5250.043.56960762-63
TCTTCTG5100.043.4715954-55
TCTGCTT5300.042.71363458-59
GTCTTCT5450.042.41089254-55
CGTCTTC5500.041.5978252-53
TTCTGCT5400.041.0420156-57
CTTCTGC5600.040.41827456-57
GCCGTCT5550.040.3743850-51
TATGCCG5700.040.1395148-49
TGCCGTC5650.040.07725550-51
ATGCCGT5650.039.65978648-49
CCGTCTT5950.039.64099552-53
CTCGTAT6300.037.80095344-45
GTATGCC6100.037.5074146-47
TCTCGTA6450.036.548742-43
ATCTCGT6700.035.5367942-43
CGTATGC6600.035.38070346-47
Produced by FastQC (version 0.11.5)