FastQCFastQC Report
Sat 23 Apr 2022
EGAF00000818609

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000818609
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2920044
Sequences flagged as poor quality0
Sequence length100
%GC27

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACGA404.656613E-946.98647328-29
CACGATC358.636562E-846.98647330-31
GTCACGG253.0160727E-546.9864728-29
TCACGAT452.5283953E-1046.9864730-31
CGGCTAC253.0169886E-546.9840632-33
ACGATCA358.640018E-846.98405532-33
CGATCAG358.640018E-846.98405532-33
CATCTCG301.6106023E-646.98164438-39
TACATCT301.6106023E-646.98164436-37
CCGTATC950.046.98164438-39
TCAGATC301.6106023E-646.98164436-37
GATCTCG404.660251E-946.98164438-39
CAGATCT301.6106023E-646.98164436-37
TCCGTAT950.046.98164436-37
AGATCTC404.660251E-946.98164438-39
GATCAGA358.643474E-846.9816434-35
GTATCTC1050.044.74442340-41
TATCTCG1050.044.74442340-41
CGTATCT1050.044.74442338-39
TTCCGTA1000.044.63256536-37
Produced by FastQC (version 0.11.5)