FastQCFastQC Report
Sat 23 Apr 2022
EGAF00000818755

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000818755
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Sequences flagged as poor quality0
Sequence length100
%GC25

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTGAA1950.045.79214558-59
TTCTGCT2150.042.6253754-55
CTGCTTG2100.042.52127556-57
TCTGCTT2050.042.41210656-57
ATGCCGT2350.040.99755546-47
TCTTCTG2250.040.73090752-53
GCCGTCT2400.040.14344448-49
CGTCTTC2500.039.47764650-51
GTCTTCT2400.039.16433752-53
CTTGAAA2300.038.82377660-61
TGCTTGA2200.038.45225558-59
CCGTCTT2500.037.5977650-51
TGCCGTC2450.037.40593348-49
TATGCCG2450.037.40593346-47
GTATGCC2600.037.05548544-45
TCTCGTA2750.035.8887740-41
CTCGTAT2700.034.8127442-43
CTTCTGC2600.033.44031554-55
AATCTCG3000.032.89803738-39
ATCTCGT2900.032.4118640-41
Produced by FastQC (version 0.11.5)