FastQCFastQC Report
Sat 23 Apr 2022
EGAF00000818871

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000818871
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Sequences flagged as poor quality0
Sequence length100
%GC26

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCC53420.13355TruSeq Adapter, Index 3 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC50310.125775TruSeq Adapter, Index 10 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAATGC156.904703E-493.995894
TACTACA156.904703E-493.995894
AACACAT352.939043E-453.71188494
CACTAGT205.681411E-446.9955530-31
ACTAGTT205.681411E-446.9955532-33
TACTAGC404.649337E-946.9955530-31
CTAGCTT8100.046.4153632-33
GTCACTA7950.046.4044128-29
CACTAGC7650.046.38122630-31
TATCTCG8600.045.9026338-39
CTTATCT8350.045.8699136-37
AGCTTAT8400.045.5968734-35
ACTAGCT8050.043.2008832-33
TCACTAG7900.043.1288330-31
GCTTATC8200.042.69717436-37
CATCTCG10650.042.58282538-39
ACAGTCA2050.042.41061832-33
TCACAGT2000.042.29599430-31
TTATCTC8650.042.1058438-39
CTGCTTT950.042.0486556-57
Produced by FastQC (version 0.11.5)