FastQCFastQC Report
Sat 23 Apr 2022
EGAF00000818872

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000818872
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Sequences flagged as poor quality0
Sequence length100
%GC25

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTGGGA406.719347E-945.0771331
GAACGGA850.045.0771331
GATTGGA253.853378E-545.0771331
GAGAGCG1650.044.9908566
AAAGCGT850.044.9908568
AAAGCGC207.0424326E-444.9908568
GGAGAGC1650.044.9852265
TTGGGAG207.0528546E-444.9773522
ACGGAAG351.2177406E-744.9773523
GATCGGA5400.044.242371
TCGGGAG4050.043.86682
ATCGGGA4850.043.6831
AAAAGCG1650.043.62757
CGGGAGA4250.042.860773
AAGCGTC900.042.4913679
GAAAAGC1750.041.1344956
GAGGGGC3000.040.491778
GGGAGAG4750.040.247424
TCGGAAA1700.039.6858983
CCGAAGA356.2689705E-638.556364
Produced by FastQC (version 0.11.5)