FastQCFastQC Report
Sat 23 Apr 2022
EGAF00000819217

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000819217
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Sequences flagged as poor quality0
Sequence length101
%GC26

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGAA308.27767E-539.59753820-21
GAAGAGG404.430015E-429.76255494-95
GTCTTGT458.8976714E-426.41653492-93
CTTGTAG500.001630366223.81004594-95
CAAATAG550.00283697621.64549694-95
TGGGGGG550.002880479421.58880240-41
GGGAAAA951.8624934E-417.50606222-23
CACAAGA850.0017918616.76045290-91
TACTAGA850.0017918616.76045288-89
ATACAGA900.002576670115.87336494-95
GTAAGAG1206.023223E-515.87336394-95
ATTAAAG900.002624879415.82931688-89
CTCGGTG2900.015.59968594-95
TATAGAT1552.012859E-615.361320594-95
TCGTAAG1258.643609E-515.21592392-93
TTCTAGA950.003762876414.99619288-89
AGCATCG1000.00519389614.28602794-95
GGTGGTC1858.092193E-714.15732494-95
TCTCAGA1200.00110488213.85065190-91
AAAGCGT1050.007176090513.60574194-95
Produced by FastQC (version 0.11.5)