FastQCFastQC Report
Sat 23 Apr 2022
EGAF00000820933

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000820933
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Sequences flagged as poor quality0
Sequence length101
%GC25

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTCGG156.6350313E-494.942691
ATTCGGG205.384887E-447.50935436-37
TCGGGGG354.304815E-640.7261338-39
GGGCGCC308.281293E-539.594642-43
GGGTAGA250.001620692837.9794528-29
AGGGTAG250.001620692837.9794528-29
TTCGGGG300.003946725331.67587938-39
ATAACAG404.4855653E-429.69929190-91
GGGGAAG350.00842079127.1254722-23
GGGGGGA958.30454E-619.98793620-21
GGGGGAA757.731599E-418.99815830-31
TAACAGA1152.053168E-618.59434190-91
AGATCTC1959.4772986E-814.61442594-95
AATTAGA1705.3666354E-613.97613890-91
GTAGATC2307.0568785E-712.3926692-93
ATCTCGG1400.003515356211.8742294-95
TAGATCT2309.546926E-611.35794994-95
AATAAGA2401.530672E-510.88470294-95
AGGGAAA5550.010.69749594-95
GGAAAGA4108.403731E-1010.42614594-95
Produced by FastQC (version 0.11.5)