FastQCFastQC Report
Sat 23 Apr 2022
EGAF00000821082

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000821082
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Sequences flagged as poor quality0
Sequence length100
%GC27

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT75320.1883TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACAT255.556545E-575.2123394
TAGGCTA205.687123E-446.9859752-53
GTCTTCT9650.045.28181554-55
TCTGCTT9550.045.01796758-59
CTTGAAA9100.044.9276762-63
TGCTTGA9050.044.9092460-61
CTTCTGC9550.044.7719756-57
TCTTCTG9400.044.48671754-55
CTGCTTG8950.044.36105358-59
CCGTCTT9800.044.34952-53
GCTTGAA8900.044.3463160-61
TTCTGCT9450.044.2513456-57
TGCCGTC9700.043.8544250-51
CGTCTTC9700.043.8374252-53
TATGCCG9800.043.4069248-49
GCCGTCT9600.043.0871750-51
ATGCCGT9750.042.66533748-49
GTATGCC9850.042.23218546-47
CTGCTTT451.3189492E-841.76530558-59
CTCGTAT10850.040.71462644-45
Produced by FastQC (version 0.11.5)