FastQCFastQC Report
Sat 23 Apr 2022
EGAF00000821539

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000821539
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Sequences flagged as poor quality0
Sequence length100
%GC27

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTCC501.4551915E-1146.9778546-47
TTCCCTC205.6919706E-446.9778550-51
CCTCTTC550.046.9778552-53
TCCGTCT404.663889E-946.9778550-51
CCCTCTT550.046.9778552-53
ATTCCCT205.6919706E-446.9778548-49
TCCCTCT205.6919706E-446.9778550-51
TGCCCTC358.6494765E-846.97784850-51
GCCCTCT358.6494765E-846.97784850-51
GCTTGAA3850.044.54133660-61
CTTGAAA4050.043.50181662-63
CTGCTTG3950.043.41316658-59
TGCTTGA3950.042.81960-61
TCTTCTG4500.041.7580954-55
TTCCGTC451.3209501E-841.75808750-51
GTCTTCT4550.041.2992154-55
ATGCCCT402.4701512E-741.1056248-49
CGTCTTC4650.040.9161952-53
TCTGCTT4600.040.342758-59
GCCGTCT4200.040.26672750-51
Produced by FastQC (version 0.11.5)