FastQCFastQC Report
Sat 23 Apr 2022
EGAF00000821543

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000821543
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Sequences flagged as poor quality0
Sequence length100
%GC27

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCTTT253.015917E-546.9871558-59
CTTGAAA4050.044.66735562-63
TTCTGCT4050.044.66344556-57
GCTTGAA3750.044.4817260-61
CTGCTTG3750.044.48116358-59
TCTTCTG4100.044.11380854-55
TCTGCTT4150.043.59048558-59
TGCTTGA4000.042.2889660-61
CTTCTGC4300.042.0667356-57
GCCGTCT4300.042.06147450-51
GTATGCC4450.041.69935246-47
TGCCGTC4400.041.6393750-51
TATGCCG4400.041.6393748-49
ATGCCGT4350.041.57800748-49
GTCTTCT4750.041.5387454-55
CCGTCTT4750.041.5382252-53
CGTCTTC4700.041.48035452-53
CTCGTAT5100.040.0692644-45
CGTATGC4750.039.0657146-47
TCTCGTA5300.038.1140242-43
Produced by FastQC (version 0.11.5)