FastQCFastQC Report
Sat 23 Apr 2022
EGAF00000822058

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000822058
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Sequences flagged as poor quality0
Sequence length100
%GC26

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACCTC156.90171E-494.0060594
CTGCTTG3550.046.35434358-59
TCTGCTT4250.045.9102858-59
TTCTGCT4200.045.8968256-57
TGCTTGA3600.045.71053360-61
CTTCTGC4300.045.37615256-57
TCTTCTG4300.044.80535554-55
GTCTTCT4900.044.5934854-55
CTTGAAA3850.044.57301762-63
GCTTGAA3600.044.4045260-61
TGCCGTC4500.043.3401950-51
GCCGTCT4450.043.2991250-51
CCGTCTT5000.043.23575652-53
CGTCTTC5000.043.23575652-53
ATGCCGT4600.041.88719648-49
TATGCCG4750.041.05912848-49
GTATGCC4600.040.85380646-47
CTGCTTT655.456968E-1239.78323458-59
TCCGTCT503.3340257E-837.59630650-51
GAGTACA500.001704399637.591619
Produced by FastQC (version 0.11.5)