FastQCFastQC Report
Sat 23 Apr 2022
EGAF00000822066

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000822066
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Sequences flagged as poor quality0
Sequence length100
%GC26

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTATC301.6088889E-646.98922338-39
TCTGCTT4300.045.38353758-59
CTGCTTG3550.045.0369858-59
TGCTTGA3650.044.44725860-61
TTCTGCT4400.043.2863556-57
TGCCGTC4800.043.08853550-51
CTTGAAA4100.043.0002662-63
TATGCCG4850.042.64432548-49
GTCTTCT5350.042.6062354-55
CTTCTGC4600.042.4266656-57
TCTTCTG4500.042.29876354-55
GCTTGAA3800.042.0740260-61
ATGCCGT4850.041.67513748-49
GCCGTCT4850.041.67513750-51
CCGTCTT5500.041.44994452-53
CTGCTTT750.040.7540558-59
GTATGCC4950.040.34176346-47
AGTTCCT354.6484274E-640.27345734-35
CGTCTTC5550.040.2295852-53
CTTCTTC950.039.60180356-57
Produced by FastQC (version 0.11.5)