FastQCFastQC Report
Sat 23 Apr 2022
EGAF00000822567

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000822567
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3924327
Sequences flagged as poor quality0
Sequence length100
%GC25

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN95340.2429461153466569No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATTGGA156.9036806E-493.999252
AAAGGGC200.002159491970.531899
GAATTGG200.002163437470.499441
GAGAGGG452.1976666E-762.695027
CGGAAAG408.031209E-658.7577935
GAGGGGC451.6103548E-552.245859
GGAGAGG558.8084744E-751.279536
ATTCGGG252.9946166E-547.04352636-37
ATCCGGG205.652902E-447.0435236-37
TCCGGGG550.047.0435238-39
TAGGGGA205.656111E-447.03810518-19
GTTGGGG205.656111E-447.03810518-19
GCCCGGT252.9990339E-547.0317946-47
AAGGGGC555.2713487E-542.7466059
TTGGGGG451.3047611E-841.81646738-39
GGGCCCG451.3062163E-841.81111544-45
TCGGGGG750.040.77105338-39
GGGGCGC354.6133573E-640.3178642-43
CTGGGGG308.780774E-539.20293438-39
TGGGGAA609.640644E-1139.18964820-21
Produced by FastQC (version 0.11.5)