FastQCFastQC Report
Sat 23 Apr 2022
EGAF00000823869

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000823869
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Sequences flagged as poor quality0
Sequence length101
%GC26

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAAG301.2358214E-894.966875
GGATCGG254.6399873E-794.940751
AAGGGGC505.5952114E-966.4718259
TGGGAGG250.00502912256.9801255
TTTCGGG357.614835E-847.74464436-37
TGGAAAA405.399067E-447.4834375
GTATGGA205.4055534E-447.47245870-71
GGGTTGC205.4087193E-447.4668242-43
GGTGCCC205.409886E-447.46474544-45
GGGCCCC506.4210326E-1042.71826644-45
TCCGGGG451.2001692E-842.21383738-39
AGGGGCG402.2735185E-741.54229410-11
TTCGGGG700.040.706238-39
CTGGGGG354.3193195E-640.706238-39
TTCCGGG308.0478545E-539.78720536-37
ATTCCGG308.0478545E-539.78720536-37
GGTGGGA308.29084E-539.58685718-19
CGGGTGG250.001616629137.99886316-17
TCTGGGG250.001617970637.99244738-39
GGGGTGC250.001621950937.97345442-43
Produced by FastQC (version 0.11.5)