FastQCFastQC Report
Wed 8 May 2024
EGAF00000828774

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000828774
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13862079
Total Bases415.8 Mbp
Sequences flagged as poor quality0
Sequence length30
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGATGAAGCACTGTAGCTATCTCGTATG11340708.181096067913044No Hit
ATCTCGTATGCCGTCTTCTGCTTGAAAAAA8095365.839932090994432Illumina Paired End PCR Primer 2 (100% over 24bp)
TGAGATGAAGCACTGTAGCTCTATCTCGTA7577245.466164202353774No Hit
TGTAAACATCCTCGACTGGAAGATCTCGTA5418713.9090168220798622No Hit
AAGCTGCCAGTTGAAGAACTGTATCTCGTA4961513.579196165308249No Hit
TAGCTTATCAGACTGATGTTGAATCTCGTA4259113.0724900644412716No Hit
TGAGATGAAGCACTGTAGCTCATCTCGTAT2237461.6140868912953101No Hit
TCAGTGCACTACAGAACTTTGTATCTCGTA2095171.5114399506740654No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGT1750041.2624657527922039No Hit
TGTAAACATCCTCGACTGGAAGCATCTCGT1552681.120091726500765No Hit
TAGCACCATCTGAAATCGGTTATCTCGTAT1483661.0703012152794686No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC1178890.8504424192071045No Hit
TACCCTGTAGAACCGAATTTGTATCTCGTA1170110.8441085929462673No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTA1129670.8149354797357597No Hit
TACAGTACTGTGATAACTGAATCTCGTATG907180.6544328596020842No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTA792670.5718262029815296No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTAT780030.5627078016219645No Hit
TCAGTGCACTACAGAACTTTGAATCTCGTA709190.5116043560276925No Hit
CACCCGTAGAACCGACCTTGCATCTCGTAT680790.49111680866917584No Hit
TAATACTGCCGGGTAATGATGGAATCTCGT676390.48794268161363097No Hit
CACCCGTAGAACCGACCTTGCGATCTCGTA601880.43419172549802953No Hit
TAGCTTATCAGACTGATGTTGATCTCGTAT600930.43350640261103696No Hit
TTTGGCAATGGTAGAACTCACAATCTCGTA578010.4169720862216988No Hit
TCAGTGCACTACAGAACTTTGATCTCGTAT573770.41391338196817373No Hit
TGAGGTAGTAGGTTGTATAGATCTCGTATG558940.4032151310059624No Hit
TCCCTGGTCTAGTGGTTAGGATTCGGCGCA557920.4024793106430861No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTA555400.40066140151127405No Hit
TGAGATGAAGCACTGTAGCTAATCTCGTAT524090.378074601941022No Hit
AACCCGTAGATCCGAACTTGTATCTCGTAT522450.3768915182203189No Hit
TAGCACCATTTGAAATCGGTTATCTCGTAT513550.37047112485796685No Hit
TGAGATGAAGCACTGTAGCTCAATCTCGTA471190.3399129380232215No Hit
CTTTCAGTCGGATGTTTGCAGCATCTCGTA467320.33712114899936724No Hit
AACCCGTAGATCCGAACTTGTGAATCTCGT459600.3315519988019113No Hit
GCATGTGGTTCAGTGGTAGAATTCTCGCAT453640.3272524994266733No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCA441730.3186607146013235No Hit
TAATACTGCCGGGTAATGATGGATCTCGTA432240.3118146996565234No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCG413940.29861321667550733No Hit
TGAGGTAGTAGATTGTATAGTATCTCGTAT405740.2926977980719919No Hit
CTTTCAGTCGGATGTTTGCAGATCTCGTAT405340.2924092410669424No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTA400440.28887441775508566No Hit
GTACAGTACTGTGATAACTGAATCTCGTAT391550.28246123831785985No Hit
TGAGATGAAGCACTGTAGCATCTCGTATGC389930.28129258244740923No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTA379710.27391995096839367No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGC358890.2589005588555656No Hit
TACAGTACTGTGATAACTGAATATCTCGTA346390.24988315244776774No Hit
TCGTACCGTGAGTAATAATGCGATCTCGTA345430.24919061563564887No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTG329110.237417489829628No Hit
TACCCTGTAGATCCGAATTTGTATCTCGTA307130.22156128240215628No Hit
TAGCTTATCAGACTGATGTTGACATCTCGT306750.2212871532473592No Hit
TTTCAGTCGGATGTTTGCAGCATCTCGTAT304640.21976501504572293No Hit
TGTAAACATCCTTGACTGGATCTCGTATGC300690.2169155146208588No Hit
TGAGATGAAGCACTGTAGCTCTAATCTCGT274300.197877966212716No Hit
TGTAAACATCCTCGACTGGAAATCTCGTAT271980.1962043355834287No Hit
TGAGATGAAGCACTGTAGCAATCTCGTATG265200.19131329434783917No Hit
TGAGATGAAGCACTGTAGCTCTTATCTCGT259020.1868550886198239No Hit
TAGCACCATCTGAAATCGGTTAATCTCGTA236210.1704001254068744No Hit
AGCAGCATTGTACAGGGCTATGATCTCGTA234550.16920261383591884No Hit
TGAGGTAGTAGGTTGTATAGAATCTCGTAT231730.16716828695031966No Hit
TGAGATGAAGCACTGTAGATCTCGTATGCC230010.16592749182860667No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTG222380.16042326695728687No Hit
TTTGGCAATGGTAGAACTCACACTATCTCG206720.1491262602095977No Hit
AAGCTGCCAGTTGAAGACCTGTATCTCGTA205950.14857078797487736No Hit
TTCTCGTATGCCGTCTTCTGCTTGAAAAAA205560.14828944489495408TruSeq Adapter, Index 20 (100% over 24bp)
TGAGGTAGTAGGTTGTGTGGATCTCGTATG203840.14704864977324109No Hit
TATTGCACTTGTCCCGGCCTGTATCTCGTA190790.13763447748350013No Hit
TGTAAACATCCTCGACTGGAAGAATCTCGT188590.13604741395572773No Hit
TGTCAACATCCTCGACTGGAAGATCTCGTA187820.13549194172100737No Hit
TACAGTACTGTGATAACTGAAGATCTCGTA187720.135419802469745No Hit
TAGCACCATTTGAAATCGGTATCTCGTATG182750.13183448168200457No Hit
TATGGCACTGGTAGAATTCACTATCTCGTA174610.12596234662924657No Hit
TGAGGTAGTAGGTTGTATGGTTATCTCGTA170950.12332205003304339No Hit
TTTGGCAATGGTAGAACTCACATCTCGTAT166830.12034991288103322No Hit
AACATTCAACGCTGTCGGTGAGATCTCGTA162630.11732006432801313No Hit
CTTTCAGTCGGATGTTTACAGTATCTCGTA162320.11709643264909975No Hit
TAGCACCATTTGAAATCGGTTAATCTCGTA160570.11583399575200806No Hit
TTCAAGTAATCCAGGATAGGATCTCGTATG158800.11455713100466387No Hit
TGTAAACATCCTTGACTGGAAGATCTCGTA157110.1133379776583296No Hit
CTAGCACCATCTGAAATCGGTTATCTCGTA152520.11002678602538624No Hit
TTTGGCAATGGTAGAACTCAATCTCGTATG152000.10965166191882184No Hit
TGAGGTAGTAGATTGTATAGATCTCGTATG151290.10913947323485892No Hit
AAGCTGCCAGTTGAAGAACTGTAATCTCGT148550.10716285775026962No Hit
TGAGATGAAGCACTGTAGAATCTCGTATGC148050.10680216149395773No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCC144300.10409693957161836No Hit
CTTTCAGTCGGATGTTTACAGATCTCGTAT143090.10322405463134354No Hit
TTCACAGTGGCTAAGTTCTGCATCTCGTAT141360.10197604558450431No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)