FastQCFastQC Report
Wed 8 May 2024
EGAF00000828775

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000828775
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4743911
Total Bases142.3 Mbp
Sequences flagged as poor quality0
Sequence length30
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCAGCATTGTACAGGGCTATGAATCTCGT4277269.016315862586797No Hit
ATCTCGTATGCCGTCTTCTGCTTGAAAAAA3856898.13019046942491Illumina Paired End PCR Primer 2 (100% over 24bp)
TATTGCACTTGTCCCGGCCTGTATCTCGTA3484587.345373890867683No Hit
AAGCTGCCAGTTGAAGAACTGTATCTCGTA1446953.0501204596797877No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTA1408222.9684789617680436No Hit
TACCCTGTAGATCCGAATTTGTATCTCGTA1233562.6003017341598524No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGT1191362.5113455964920086No Hit
TAGCTTATCAGACTGATGTTGAATCTCGTA628101.3240130348145231No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGT616821.3002351856938295No Hit
TCGTTTCCGGCTCGAAGGACCAATCTCGTA361480.7619873138429452No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTA358180.7550310281959337No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTA342190.7213246622881416No Hit
AGCAGCATTGTACAGGGCTATGATCTCGTA335910.7080866399053439No Hit
CACCCGTAGAACCGACCTTGCGATCTCGTA318410.6711972463227072No Hit
CACCCGTAGAACCGACCTTGCATCTCGTAT286860.604690939606582No Hit
ACCCTGTAGATCCGAATTTGTATCTCGTAT264550.557662232702089No Hit
TGGTAGACTATGGAACGTAGGATCTCGTAT261470.551169699431545No Hit
CTAGACTGAAGCTCCTTGAGGATCTCGTAT252180.5315867013525338No Hit
TAGCTTATCAGACTGATGTTGACATCTCGT243200.5126571725312722No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTA232250.4895749519752795No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTA207730.4378876416526364No Hit
TTATGGTTTGCCTGGGACTGAATCTCGTAT162780.34313459927894935No Hit
AGCCGCATTGTACAGGGCTATGAATCTCGT157820.3326790911549563No Hit
TATTGCACTTGTCCCGGCCTGTAAATCTCG157190.33135107298598143No Hit
CTTTCAGTCGGATGTTTACAGTATCTCGTA155220.3271983812512503No Hit
TATTGCACTTGTCCCGGCCTGTTATCTCGT145400.3064981615380221No Hit
AACATTCAACGCTGTCGGTGAGATCTCGTA137760.2903933062825167No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTAT136250.287210278607672No Hit
CACTAGATTGTGAGCTCCTGGAATCTCGTA134280.2830575868729409No Hit
ACCATCGACCGTTGATTGTACCATCTCGTA132740.27981132023766886No Hit
TCAGTGCACTACAGAACTTTGTATCTCGTA130970.2760802215724536No Hit
TGTCAGTTTGTCAAATACCCCAATCTCGTA121190.25546432047312856No Hit
AACATTCAACGCTGTCGGTGAGTATCTCGT120070.25310339928383985No Hit
TACCCTGTAGATCCGAATTTGATCTCGTAT116070.24467153789352286No Hit
CATTGCACTTGTCTCGGTCTGATCTCGTAT106260.22399239783377048No Hit
TATTGCACTTGTCCCGGCCTGTATATCTCG103720.21863816585091922No Hit
TGAGATGAAGCACTGTAGCTCTATCTCGTA99000.2086885694103452No Hit
TACAGTACTGTGATAACTGAATCTCGTATG97740.20603253307239533No Hit
TTCTCGTATGCCGTCTTCTGCTTGAAAAAA95800.2019430802980916TruSeq Adapter, Index 20 (100% over 24bp)
CTTTCAGTCGGATGTTTACAGATCTCGTAT95100.2004675045547861No Hit
GTACAGTACTGTGATAACTGAATCTCGTAT91400.19266803276874292No Hit
CAAAGTGCTTACAGTGCAGGTAGATCTCGT90240.190222792965551No Hit
ACCCTGTAGATCCGAATTTGTGAATCTCGT88030.18556418954740087No Hit
TGAGATGAAGCACTGTAGCTATCTCGTATG83900.1768582926618986No Hit
TGGCTCAGTTCAGCAGGAACAGATCTCGTA82650.17422333597742454No Hit
TCGGATCCGTCTGAGCTTGGCTATCTCGTA78670.16583363389405914No Hit
ATTGCACTTGTCCCGGCCTGTATCTCGTAT77380.1631143585956819No Hit
CTAGACTGAAGCTCCTTGAGGAATCTCGTA76460.161175030475909No Hit
AACATTCAACGCTGTCGGTGAGAATCTCGT76010.16022644606949837No Hit
TCGGATCCGTCTGAGCTTGGCATCTCGTAT74290.15660074567166204No Hit
ACCACTGACCGTTGACTGTACCATCTCGTA72220.15223725740217303No Hit
ACCACAGGGTAGAACCACGGACATCTCGTA72050.15187890329308457No Hit
TCGTACCGTGAGTAATAATGCGATCTCGTA71690.15112003576795602No Hit
ACTGCAGTGAAGGCACTTGTAGATCTCGTA71120.14991849551983585No Hit
CAAAGTGCTGTTCGTGCAGGTAGAATCTCG70570.14875911457866725No Hit
TGTGACTGGTTGACCAGAGGGGATCTCGTA68020.14338380294234018No Hit
CATTGCACTTGTCTCGGTCTGTATCTCGTA64000.13490978224507164No Hit
AAGCTGCCAGTTGAAGACCTGTATCTCGTA63120.13305477273920188No Hit
TATTGCACTTGTCCCGGCCTGTAGATCTCG62660.13208510867931544No Hit
GCGGGTGATGCGAACTGGAGTCTGAGCATC62460.13166351560979958No Hit
ATCACATTGCCAGGGATTTCCAATCTCGTA61120.12883884204404342No Hit
TGAGATGAAGCACTGTAGCTCATCTCGTAT61000.1285858862023339No Hit
TGTAAACATCCCCGACTGGAAGCATCTCGT59750.12595092951785983No Hit
CGTACCGTGAGTAATAATGCGATCTCGTAT58530.12337921179381316No Hit
TACCCTGTAGAACCGAATTTGTATCTCGTA57220.12061777718848435No Hit
TGAGGTAGTAGGTTGTATAGATCTCGTATG55630.11726611228583335No Hit
ACTGGACTTGGAGTCAGAAGGCATCTCGTA54300.11446251837355297No Hit
AGCAGCATTGTACAGGGCTATGTATCTCGT53890.11359825258104546No Hit
TACAGTACTGTGATAACTGAAGATCTCGTA53410.11258642921420743No Hit
CAGCATTGTACAGGGCTATGAATCTCGTAT51990.1095931184206449No Hit
TAAAGTGCTTATAGTGCAGGTAGATCTCGT50730.10693708208269505No Hit
TACAGTACTGTGATAACTGAATATCTCGTA50450.10634685178537288No Hit
AAAAGCTGGGTTGAGAGGGCGAATCTCGTA49760.10489235569554319No Hit
ATCACATTGCCAGGGATTTCCATCTCGTAT49740.10485019638859161No Hit
TTCAAGTAATCCAGGATAGGCTATCTCGTA49320.10396485094260834No Hit
CATTGCACTTGTCTCGGTCTGAAATCTCGT47850.10086614188166683No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)