FastQCFastQC Report
Wed 8 May 2024
EGAF00000828776

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000828776
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7800915
Total Bases234 Mbp
Sequences flagged as poor quality0
Sequence length30
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTATGCCGTCTTCTGCTTGAAAAAA7184519.209829872521365Illumina Paired End PCR Primer 2 (100% over 24bp)
TACAGTACTGTGATAACTGAATCTCGTATG6155487.890715384028669No Hit
TAGCTTATCAGACTGATGTTGAATCTCGTA4270475.474319358690615No Hit
TAGCACCATCTGAAATCGGTTATCTCGTAT2345453.006634478134937No Hit
TAGCTTATCAGACTGATGTTGATCTCGTAT1960712.5134359238627777No Hit
TCTCCCAACCCTTGTACCAGTGATCTCGTA1681742.1558240283351378No Hit
TCTCCCAACCCTTGTACCAGTATCTCGTAT1548421.9849209996519641No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGT1300711.6673813264213238No Hit
TCTCCCAACCCTTGTACCAGATCTCGTATG1245581.5967101295168578No Hit
TACAGTACTGTGATAACTGAATATCTCGTA1077461.3811969493322258No Hit
TGAGGTAGTAGGTTGTATAGATCTCGTATG878041.1255602708143853No Hit
TATTGCACTTGTCCCGGCCTGTATCTCGTA876801.1239707136919195No Hit
GTACAGTACTGTGATAACTGAATCTCGTAT735130.9423638124502062No Hit
TTCAAGTAATCCAGGATAGGATCTCGTATG728350.933672524312853No Hit
TGTAAACATCCTTGACTGGATCTCGTATGC682600.8750255578992977No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGT669440.8581557419866772No Hit
TACAGTACTGTGATAACTGAAATCTCGTAT648840.8317485833392622No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTAT593800.7611927575162657No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTA582130.7462329739524145No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTA577380.7401439446526465No Hit
GCGGGTGATGCGAACTGGAGTCTGAGCATC557940.71522379105528No Hit
TAGCACCATTTGAAATCGGTTATCTCGTAT555070.7115447354573149No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTA498550.6390916962946014No Hit
TGAGGTAGTAGGTTGTATAGAATCTCGTAT482160.6180813404581386No Hit
TCAGTGCACTACAGAACTTTGAATCTCGTA471070.6038650594193117No Hit
TAGCACCATCTGAAATCGGTATCTCGTATG413700.5303224044871659No Hit
TACAGTACTGTGATAACTGAAGATCTCGTA393650.5046202913376188No Hit
TCAGTGCACTACAGAACTTTGATCTCGTAT393380.5042741780932108No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTA345610.44303777185112264No Hit
GTTTGTGATGACTTACATGGAATCTCGTAT338900.43443621677713445No Hit
TAGCACCATTTGAAATCGGTATCTCGTATG327970.4204250398831419No Hit
TGTAAACATCCTTGACTGGAAGATCTCGTA309000.39610737971122617No Hit
CACTAGATTGTGAGCTCCTGGAATCTCGTA293570.37632764874376917No Hit
TAGCACCATCTGAAATCGGTTAATCTCGTA286040.3666749349275053No Hit
GTAGTGTTTCCTACTTTATGGATCTCGTAT283500.36341890662826093No Hit
TCAGTGCACTACAGAACTTTGTATCTCGTA274700.35213817866237485No Hit
TACAGTACTGTGATAACTGAAAATCTCGTA269520.34549793197336465No Hit
TAGCACCATTTGAAATCGGATCTCGTATGC254980.3268590928115484No Hit
TGAGGTAGTAGATTGTATAGTATCTCGTAT253500.3249618794718312No Hit
AAGCTGCCAGTTGAAGAACTGTATCTCGTA242580.3109635215868908No Hit
TAGCACCATCTGAAATCGGATCTCGTATGC239330.3067973436449442No Hit
TCTCCCAACCCTTGTACCAGTGAATCTCGT230880.2959652809958832No Hit
TCGTTTCCGGCTCGAAGGACCAATCTCGTA223670.28672277546928787No Hit
AGCAGCATTGTACAGGGCTATGATCTCGTA221460.28388977446876423No Hit
CTTTCAGTCGGATGTTTACAGTATCTCGTA207800.26637900810353654No Hit
AACTTGACCGCTCTGACCAATCTCGTATGC197590.2532907998612983No Hit
CTTTCAGTCGGATGTTTACAGATCTCGTAT196920.2524319262548047No Hit
TAGCTTATCAGACTGATGTTGACATCTCGT178990.2294474430243119No Hit
TGAGGTAGTAGATTGTATAGATCTCGTATG174260.22338405174264814No Hit
CTTTCAGTCGGATGTTTACAATCTCGTATG168140.2155388182027365No Hit
TAGCACCATCTGAAATCGGTAATCTCGTAT164730.21116753611595562No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTA160370.2055784481692212No Hit
ATGTGTGACTGAAAGGTATTTTCTGAGCAT150600.19305427632527725No Hit
CGCGGGTGATGCGAACTGGAGTCTGAGCAT149980.19225949776404436No Hit
TGAGGTAGTAGGTTGTGTGGATCTCGTATG138360.1773638092454539No Hit
CTAGCACCATCTGAAATCGGTTATCTCGTA134960.1730053461677252No Hit
GTAGTGTTTCCTACTTTATGGAATCTCGTA133570.1712235039094773No Hit
TACCACAGGGTAGAACCACGGAATCTCGTA130240.1669547738951136No Hit
TTCAAGTAATCCAGGATAGGCTATCTCGTA130210.16691631686795716No Hit
TCTCCCAACCCTTGTACCAGTGTATCTCGT119400.1530589680825903No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAA116160.1489056091496959Illumina Paired End PCR Primer 2 (100% over 23bp)
TAGCTTATCAGACTGATGTTGAAATCTCGT104610.13409965369447047No Hit
ATCGCGTATGCCGTCTTCTGCTTGAAAAAA98710.12653643835370595Illumina Paired End PCR Primer 2 (95% over 24bp)
TGAGGTAGTAGATTGTATAGAATCTCGTAT97590.1251007093398659No Hit
TAGCACCATTTGAAATCAGTGATCTCGTAT96980.12431874978768517No Hit
ATGCAGTGTGGAACACAATGAACTGAACAT96360.12352397122645227No Hit
AATGTGTGACTGAAAGGTATTTTCTGAGCA95830.12284456374668869No Hit
TTAATGCTAATCGTGATAGGGATCTCGTAT95380.12226770833934225No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGT93460.11980645860133073No Hit
TAGCACCATCTGAAATCGGTTAAATCTCGT93260.11955007842028788No Hit
TAGCACCATTTGAAATCGGTTAATCTCGTA92880.11906295607630643No Hit
TAGCAGCACGTAAATATTGGCGATCTCGTA91220.11693500057365065No Hit
TAGCACCATTTGAAATCAGTGTTATCTCGT90710.11628123111199135No Hit
TTAATGCTAATCGTGATAGGGGATCTCGTA90450.11594793687663564No Hit
TATTGCACTTGTCCCGGCCTGTTATCTCGT90060.11544799552360205No Hit
GTCCAGTACTGTGATAACTGAATCTCGTAT85840.1100383737035976No Hit
TAGCTTATCAGACTGATGTTATCTCGTATG83200.10665415531383178No Hit
CACTAGATTGTGAGCTCCTGGAAATCTCGT81090.10394934440382955No Hit
TAAAGTACTGTGATAACTGAATCTCGTATG80890.10369296422278668No Hit
TGTAGTGTTTCCTACTTTATGGATCTCGTA80730.1034878600779524No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)