FastQCFastQC Report
Wed 8 May 2024
EGAF00000828777

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000828777
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6600673
Total Bases198 Mbp
Sequences flagged as poor quality0
Sequence length30
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTATGCCGTCTTCTGCTTGAAAAAA6376469.660317970606936Illumina Paired End PCR Primer 2 (100% over 24bp)
TAGCTTATCAGACTGATGTTGAATCTCGTA5494918.324772337608605No Hit
TAGCTTATCAGACTGATGTTGATCTCGTAT2319803.5144901133566226No Hit
TACAGTACTGTGATAACTGAATCTCGTATG2170753.2886798058319204No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGT1589582.4082089811145018No Hit
TAGCACCATCTGAAATCGGTTATCTCGTAT1072161.624319217146494No Hit
TCTCCCAACCCTTGTACCAGTGATCTCGTA1018251.5426457271856975No Hit
AAGCTGCCAGTTGAAGAACTGTATCTCGTA1007481.5263292091579146No Hit
TGAGGTAGTAGGTTGTATAGATCTCGTATG895951.3573615902499638No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTA783241.1866062748450046No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTA617320.9352379674012029No Hit
TCTCCCAACCCTTGTACCAGTATCTCGTAT602090.9121645626135396No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTA578130.8758652337420745No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTAT565750.8571095704937967No Hit
TATTGCACTTGTCCCGGCCTGTATCTCGTA516140.7819505677678624No Hit
GTACAGTACTGTGATAACTGAATCTCGTAT493880.748226733849715No Hit
TCGTTTCCGGCTCGAAGGACCAATCTCGTA486200.7365915566488447No Hit
TCTCCCAACCCTTGTACCAGATCTCGTATG477290.7230929330993976No Hit
AGCAGCATTGTACAGGGCTATGATCTCGTA448730.6798246178836612No Hit
TACAGTACTGTGATAACTGAATATCTCGTA403410.6111649524222758No Hit
TACAGTACTGTGATAACTGAAATCTCGTAT372130.5637758452812311No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTA351420.5324002567616969No Hit
TGAGGTAGTAGGTTGTGTGGATCTCGTATG332470.503691063017362No Hit
GTTTGTGATGACTTACATGGAATCTCGTAT327330.49590397827615457No Hit
TTCAAGTAATCCAGGATAGGATCTCGTATG325470.4930860837978188No Hit
TGTAAACATCCTTGACTGGAAGATCTCGTA302620.45846840163116703No Hit
TAGCTTATCAGACTGATGTTGACATCTCGT297410.45057526709776413No Hit
GCGGGTGATGCGAACTGGAGTCTGAGCATC297310.45042376739462775No Hit
TCAGTGCACTACAGAACTTTGATCTCGTAT295620.4478634224116238No Hit
GTAGTGTTTCCTACTTTATGGATCTCGTAT291880.442197333514325No Hit
TTAATGCTAATCGTGATAGGGATCTCGTAT270520.40983699692440456No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGT266560.40383760868020574No Hit
TGAGGTAGTAGGTTGTATAGAATCTCGTAT266200.403292209748915No Hit
TAGCACCATCTGAAATCGGTATCTCGTATG261210.395732374562412No Hit
TTAATGCTAATCGTGATAGGGGATCTCGTA247360.3747496656780301No Hit
TGAGGTAGTAGATTGTATAGTATCTCGTAT232590.3523731595247939No Hit
TGTAAACATCCTTGACTGGATCTCGTATGC223730.33895028582691494No Hit
GTAGTGTTTCCTACTTTATGGAATCTCGTA222230.33667779027986994No Hit
AACTTGACCGCTCTGACCAATCTCGTATGC221880.3361475413188928No Hit
TAGCACCATCTGAAATCGGATCTCGTATGC207790.3148012331469836No Hit
CTTTCAGTCGGATGTTTACAATCTCGTATG207190.31389223492816565No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGT206470.31280143706558405No Hit
TACAGTACTGTGATAACTGAAGATCTCGTA202640.3069989984354626No Hit
CTTTCAGTCGGATGTTTACAGATCTCGTAT199880.30281760662889984No Hit
TAGCACCATTTGAAATCGGTTATCTCGTAT188410.28544059067916255No Hit
TAGCACCATTTGAAATCGGATCTCGTATGC187010.28331959483525393No Hit
TGAGGTAGTAGATTGTATAGATCTCGTATG174920.26500328072607143No Hit
CTTTCAGTCGGATGTTTACAGTATCTCGTA173850.26338223390251264No Hit
TCAGTGCACTACAGAACTTTGTATCTCGTA168380.2550952001409553No Hit
TGAGGTAGTAGGTTGTGTGGTATCTCGTAT155360.23536993879260493No Hit
TAGCACCATTTGAAATCGGTATCTCGTATG149470.22644660627787497No Hit
TAGCTTATCAGACTGATGTTATCTCGTATG146370.2217501154806487No Hit
GGAGAGAACGCGGTCTGAGTGGATCTCGTA145160.21991696907269911No Hit
TTAATGCTAATCGTGATAGGGGTATCTCGT143320.2171293745349906No Hit
TCAGTGCACTACAGAACTTTGAATCTCGTA141360.21415998035351852No Hit
TGAGGTAGTAGGTTGTGTGGTTATCTCGTA141150.2138418309769322No Hit
TGAGGTAGTAGGTTGTGTGAATCTCGTATG129910.19681326434440855No Hit
TGTAGTGTTTCCTACTTTATGGATCTCGTA128520.19470741847081352No Hit
TTAATGCTAATCGTGATAGGATCTCGTATG121760.1844660385387975No Hit
TAGCAGCACGTAAATATTGGCGATCTCGTA119460.18098154536666186No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTA117660.17825455071020788No Hit
TACCACAGGGTAGAACCACGGAATCTCGTA110820.1678919710156828No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAA105410.15969583707600724Illumina Paired End PCR Primer 2 (100% over 23bp)
TTAATGCTAATCGTGATAGGGAATCTCGTA105000.15907468829314828No Hit
CACTAGATTGTGAGCTCCTGGAATCTCGTA96350.1459699639718556No Hit
TAGCACCATCTGAAATCGGTTAATCTCGTA96200.1457427144171511No Hit
TAGCTTATCAGACTGATGTTGAAATCTCGT94400.14301571976069713No Hit
TCTCCCAACCCTTGTACCAGTGAATCTCGT83510.1265174020891506No Hit
TACAGTACTGTGATAACTGAAAATCTCGTA81720.12380555740301026No Hit
TAGCACCATTTGAAATCAGTGATCTCGTAT81400.123320758352974No Hit
CTAGCACCATCTGAAATCGGTTATCTCGTA78350.1187000174073159No Hit
ATCGCGTATGCCGTCTTCTGCTTGAAAAAA74500.11286727883656712Illumina Paired End PCR Primer 2 (95% over 24bp)
TTAATGCTAATCGTGATAGGGGAATCTCGT74300.11256427943029447No Hit
TTCTCGTATGCCGTCTTCTGCTTGAAAAAA72070.10918583605035426TruSeq Adapter, Index 20 (100% over 24bp)
CGCGGGTGATGCGAACTGGAGTCTGAGCAT68850.10430754560936438No Hit
TAGCACCATCTGAAATCGGTAATCTCGTAT67110.1016714507747922No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)