FastQCFastQC Report
Wed 8 May 2024
EGAF00000828778

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000828778
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6310080
Total Bases189.3 Mbp
Sequences flagged as poor quality0
Sequence length30
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTATGCCGTCTTCTGCTTGAAAAAA64536010.227445610832191Illumina Paired End PCR Primer 2 (100% over 24bp)
TAGCTTATCAGACTGATGTTGAATCTCGTA3902326.184263907906081No Hit
TACAGTACTGTGATAACTGAATCTCGTATG3545245.618375678279832No Hit
TAGCTTATCAGACTGATGTTGATCTCGTAT1840972.9175065926264008No Hit
TCTCCCAACCCTTGTACCAGTGATCTCGTA1408092.231493103098534No Hit
TAGCACCATCTGAAATCGGTTATCTCGTAT1274412.019641589330088No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGT1162941.842987727572392No Hit
TCTCCCAACCCTTGTACCAGTATCTCGTAT811941.286734874993661No Hit
TACAGTACTGTGATAACTGAATATCTCGTA765491.2131224960697804No Hit
TGAGGTAGTAGGTTGTATAGATCTCGTATG683351.0829498199705867No Hit
AAGCTGCCAGTTGAAGAACTGTATCTCGTA652391.0338854657944117No Hit
TATTGCACTTGTCCCGGCCTGTATCTCGTA595590.9438707591662864No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTA572120.9066763020437142No Hit
GTACAGTACTGTGATAACTGAATCTCGTAT547020.8668986764034688No Hit
TCTCCCAACCCTTGTACCAGATCTCGTATG521780.8268991835285766No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTAT509390.8072639332623359No Hit
TTCAAGTAATCCAGGATAGGATCTCGTATG482210.7641899944216238No Hit
TACAGTACTGTGATAACTGAAATCTCGTAT471970.7479619909731731No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTA448370.7105615142755718No Hit
AACTTGACCGCTCTGACCAATCTCGTATGC410510.6505622749632335No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTA394600.6253486485115878No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTA346590.5492640346873573No Hit
GCGGGTGATGCGAACTGGAGTCTGAGCATC329720.5225290329124195No Hit
TGAGGTAGTAGGTTGTATAGAATCTCGTAT327220.5185671180080126No Hit
TCAGTGCACTACAGAACTTTGATCTCGTAT325020.5150806328921345No Hit
TCAGTGCACTACAGAACTTTGAATCTCGTA321820.5100093818144936No Hit
TGTAAACATCCTTGACTGGATCTCGTATGC312870.49582572645671685No Hit
GTAGTGTTTCCTACTTTATGGATCTCGTAT300800.47669760129824024No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGT292720.4638926923271971No Hit
TAGCTTATCAGACTGATGTTGACATCTCGT288440.4571098940108525No Hit
TACAGTACTGTGATAACTGAAGATCTCGTA279450.4428628480146052No Hit
GTTTGTGATGACTTACATGGAATCTCGTAT278230.4409294335412546No Hit
TCAGTGCACTACAGAACTTTGTATCTCGTA241150.3821663116790912No Hit
TGTAGTGTTTCCTACTTTATGGATCTCGTA225560.3574598103352097No Hit
TGAGGTAGTAGATTGTATAGTATCTCGTAT221430.35091472691312947No Hit
TGTAAACATCCTTGACTGGAAGATCTCGTA220540.34950428520716065No Hit
TAGCACCATCTGAAATCGGTATCTCGTATG204810.3245759166286323No Hit
TCTCCCAACCCTTGTACCAGTGAATCTCGT193580.3067789948780364No Hit
GTAGTGTTTCCTACTTTATGGAATCTCGTA186810.2960501293169025No Hit
AGCAGCATTGTACAGGGCTATGATCTCGTA178960.2836097165170648No Hit
TACAGTACTGTGATAACTGAAAATCTCGTA173660.2752104569197221No Hit
CTTTCAGTCGGATGTTTACAGATCTCGTAT172170.27284915563669554No Hit
CTTTCAGTCGGATGTTTACAGTATCTCGTA170510.2702184441401694No Hit
TAGCACCATCTGAAATCGGTTAATCTCGTA168280.2666844160454384No Hit
TAGCACCATTTGAAATCGGTTATCTCGTAT163660.25936279730209444No Hit
TCGTTTCCGGCTCGAAGGACCAATCTCGTA150050.2377941325625032No Hit
TTAATGCTAATCGTGATAGGGGATCTCGTA149130.23633614787768142No Hit
TTAATGCTAATCGTGATAGGGGTATCTCGT148280.23498909681018307No Hit
CTTTCAGTCGGATGTTTACAATCTCGTATG139710.22140765251787617No Hit
TGAGGTAGTAGATTGTATAGATCTCGTATG137300.2175883665500279No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTA131330.20812731375830415No Hit
TTAATGCTAATCGTGATAGGGATCTCGTAT128530.20368996906536843No Hit
CACTAGATTGTGAGCTCCTGGAATCTCGTA121080.19188346265023581No Hit
TCTCCCAACCCTTGTACCAGTGTATCTCGT120450.19088506009432527No Hit
TAGCACCATCTGAAATCGGATCTCGTATGC120440.19086921243470764No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGT119240.1889674932805923No Hit
TGTAGTGTTTCCTACTTTATGGAATCTCGT116870.18521159795121458No Hit
TAGCAGCACGTAAATATTGGCGATCTCGTA108900.1725810132359653No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAA107440.17026725493179168Illumina Paired End PCR Primer 2 (100% over 23bp)
TTCAAGTAATCCAGGATAGGCTATCTCGTA100140.15869846341092347No Hit
CTAGCACCATCTGAAATCGGTTATCTCGTA99300.15736726000304277No Hit
TAGCTTATCAGACTGATGTTGAAATCTCGT97770.15494256808154572No Hit
TAGCTTATCAGACTGATGTTATCTCGTATG93080.14751001572087832No Hit
TTAATGCTAATCGTGATAGGGAATCTCGTA91430.14489515188396979No Hit
ATCGCGTATGCCGTCTTCTGCTTGAAAAAA89890.1424546123028551Illumina Paired End PCR Primer 2 (95% over 24bp)
TGAGGTAGTAGGTTGTGTGGATCTCGTATG88350.14001407272174046No Hit
CGCGGGTGATGCGAACTGGAGTCTGAGCAT85200.13502205994218774No Hit
TAGCACCATTTGAAATCGGTATCTCGTATG84100.13327881738424868No Hit
TTCTCGTATGCCGTCTTCTGCTTGAAAAAA77250.12242317054617373TruSeq Adapter, Index 20 (100% over 24bp)
TAGCACCATCTGAAATCGGTAATCTCGTAT76420.12110781479791063No Hit
TTCAAGTAATTCAGGATAGGTTATCTCGTA71660.11356432881991986No Hit
TGTAAACATCCTTGACTGGAAGCATCTCGT70120.11112378923880521No Hit
TAGCACCATTTGAAATCGGATCTCGTATGC69900.1107751407272174No Hit
TGAGGTAGTAGGTTGTGTGGTTATCTCGTA67960.10770069476139764No Hit
TGAGGTAGTAGATTGTATAGAATCTCGTAT66700.10570388964957655No Hit
TAGCACCATTTGAAATCAGTGATCTCGTAT66480.10535524113798873No Hit
TTAATGCTAATCGTGATAGGGGAATCTCGT66140.1048164207109894No Hit
GTCCAGTACTGTGATAACTGAATCTCGTAT65950.10451531517825446No Hit
ATAGCGTATGCCGTCTTCTGCTTGAAAAAA63920.10129824027587606Illumina Single End Adapter 1 (95% over 21bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)