FastQCFastQC Report
Wed 8 May 2024
EGAF00000828779

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000828779
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34493328
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length30
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGATGAAGCACTGTAGCTCTATCTCGTA30223138.762022035101976No Hit
TGAGATGAAGCACTGTAGCTATCTCGTATG30166088.745482604635887No Hit
ATCTCGTATGCCGTCTTCTGCTTGAAAAAA16321714.731845532562123Illumina Paired End PCR Primer 2 (100% over 24bp)
AAGCTGCCAGTTGAAGAACTGTATCTCGTA16241194.7085018876694065No Hit
TGTAAACATCCTCGACTGGAAGATCTCGTA13338403.866950733196867No Hit
TGAGATGAAGCACTGTAGCTCATCTCGTAT8386962.4314731243097216No Hit
TCAGTGCACTACAGAACTTTGTATCTCGTA8140782.360102800170514No Hit
TAGCTTATCAGACTGATGTTGAATCTCGTA7411792.1487604791280215No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGT6784521.9669079191198948No Hit
TGTAAACATCCTCGACTGGAAGCATCTCGT4657801.3503481021025283No Hit
TACAGTACTGTGATAACTGAATCTCGTATG4651071.3483970001386936No Hit
TACCCTGTAGAACCGAATTTGTATCTCGTA3399470.9855442188703856No Hit
CACCCGTAGAACCGACCTTGCGATCTCGTA2971770.8615492248240008No Hit
CACCCGTAGAACCGACCTTGCATCTCGTAT2969090.8607722629721318No Hit
AACCCGTAGATCCGAACTTGTATCTCGTAT2770340.8031524241441707No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTA2607560.7559606889773002No Hit
TAGCACCATCTGAAATCGGTTATCTCGTAT2485710.7206350167197552No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTA2443460.7083862711072704No Hit
GTACAGTACTGTGATAACTGAATCTCGTAT2215010.6421560714582252No Hit
TCAGTGCACTACAGAACTTTGATCTCGTAT2087890.6053025674994306No Hit
TCAGTGCACTACAGAACTTTGAATCTCGTA2079170.6027745423694693No Hit
AACCCGTAGATCCGAACTTGTGAATCTCGT2064170.5984258752881136No Hit
TGAGATGAAGCACTGTAGCTCAATCTCGTA1938790.5620768167107564No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTA1886600.5469463543790265No Hit
TGAGATGAAGCACTGTAGCTAATCTCGTAT1768750.5127803266765097No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTA1715960.49747591766152577No Hit
TGAGGTAGTAGATTGTATAGTATCTCGTAT1629720.47247398105511884No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTAT1517360.43989956550437814No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTA1486240.43087753086625913No Hit
CTTTCAGTCGGATGTTTGCAGCATCTCGTA1376100.398946718043559No Hit
TAATACTGCCGGGTAATGATGGAATCTCGT1276530.3700802659575208No Hit
AGCAGCATTGTACAGGGCTATGATCTCGTA1108940.3214940582132289No Hit
TGTAAACATCCTTGACTGGATCTCGTATGC1106450.32077217947772396No Hit
TGAGATGAAGCACTGTAGCATCTCGTATGC1057120.30647086300283927No Hit
TCGTACCGTGAGTAATAATGCGATCTCGTA1018500.2952744948240425No Hit
TAGCTTATCAGACTGATGTTGATCTCGTAT1015490.2944018622963838No Hit
TAATACTGCCGGGTAATGATGGATCTCGTA1005720.29156943047072753No Hit
TACAGTACTGTGATAACTGAATATCTCGTA987180.2861944779581721No Hit
TGAGATGAAGCACTGTAGCTCTAATCTCGT973700.28228647580772726No Hit
CGCGACCTCAGATCAGACGTGGATCTCGTA972170.2818429117654289No Hit
TGAGATGAAGCACTGTAGCTCTTATCTCGT957320.27753773135488696No Hit
TGAGGTAGTAGGTTGTATAGATCTCGTATG955560.2770274877506746No Hit
CTTTCAGTCGGATGTTTGCAGATCTCGTAT869480.2520719369264688No Hit
AAGCTGCCAGTTGAAGACCTGTATCTCGTA856830.2484045610211923No Hit
TACAGTACTGTGATAACTGAAGATCTCGTA848050.24585914122290548No Hit
TTTGGCAATGGTAGAACTCACAATCTCGTA844240.24475457978424117No Hit
TACCCTGTAGATCCGAATTTGTATCTCGTA804720.23329729158056306No Hit
TGAGATGAAGCACTGTAGCAATCTCGTATG788380.22856014357327306No Hit
TGCGATGAAGCACTGTAGCTCTATCTCGTA774430.2245158831876124No Hit
TGCGATGAAGCACTGTAGCTATCTCGTATG720900.2089969399299482No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC675500.19583497423037868No Hit
TATTGCACTTGTCCCGGCCTGTATCTCGTA636090.18440957625196386No Hit
TTTCAGTCGGATGTTTGCAGCATCTCGTAT634160.18385004775416278No Hit
TGTAAACATCCTCGACTGGAAGAATCTCGT627560.18193663423836634No Hit
TGTAAACATCCTCGACTGGAAATCTCGTAT626990.18177138488927483No Hit
TGAGATGAAGCACTGTAGATCTCGTATGCC615390.17840841567969318No Hit
TAGCACCATTTGAAATCGGTTATCTCGTAT597850.17332337430589476No Hit
AAACCGTTACCATTACTGAGTATCTCGTAT583370.16912546101669285No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGT548840.15911482939541235No Hit
TGTAAACATCCTTGACTGGAAGATCTCGTA540620.1567317598348295No Hit
CGCGACCTCAGATCAGACGATCTCGTATGC528630.15325572528113263No Hit
TTCAAGTAATCCAGGATAGGATCTCGTATG521250.1511161810771057No Hit
AACCCGTAGATCCGAACTTGTGATCTCGTA513300.14881138752398723No Hit
CTTTCAGTCGGATGTTTACAGTATCTCGTA487870.1414389472653958No Hit
TGAGGTAGTAGATTGTATAGATCTCGTATG466390.13521165600489463No Hit
TAGCTTATCAGACTGATGTTGACATCTCGT462910.13420276524202013No Hit
TGAGATGAAGCACTGTAGAAAATCTCGTAT448300.12996716350477983No Hit
TTCAAGTAATCCAGGATAGGCTATCTCGTA446550.129459819011955No Hit
TGAGATGAAGCACTGTAGAATCTCGTATGC440860.12781022463242747No Hit
TACAGTACTGTGATAACTGAAATCTCGTAT425870.12346445666245948No Hit
TGAGGTAGTAGGTTGTATAGAATCTCGTAT419890.12173078805269238No Hit
TGGCTCAGTTCAGCAGGAACAGTATCTCGT398110.11541652345056412No Hit
TAGCACCATCTGAAATCGGTTAATCTCGTA396290.11488888517802631No Hit
CTTTCAGTCGGATGTTTACAGATCTCGTAT395940.11478741627946135No Hit
TGGCTCAGTTCAGCAGGAACAGATCTCGTA393900.114195997556397No Hit
TGTAAACATCCTTGACTGGAAGCATCTCGT387100.11222460181284914No Hit
TGATATGAAGCACTGTAGCTATCTCGTATG379990.11016333361628661No Hit
TGATATGAAGCACTGTAGCTCTATCTCGTA366780.10633360747330613No Hit
TTCACAGTGGCTAAGTTCTGCATCTCGTAT361790.10488695089090852No Hit
TCAGTGCACTACAGAACTTTGTCATCTCGT355580.10308660271922733No Hit
AAGCTGCCAGTTGAAGAACTGTAATCTCGT352800.10228064975348276No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)