FastQCFastQC Report
Fri 10 May 2024
EGAF00000828780

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000828780
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14835494
Total Bases445 Mbp
Sequences flagged as poor quality0
Sequence length30
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGATGAAGCACTGTAGCTATCTCGTATG12967008.74052458246419No Hit
TGAGATGAAGCACTGTAGCTCTATCTCGTA11760247.92709700128624No Hit
ATCTCGTATGCCGTCTTCTGCTTGAAAAAA9608436.4766498506891645Illumina Paired End PCR Primer 2 (100% over 24bp)
AAGCTGCCAGTTGAAGAACTGTATCTCGTA5287313.563959514930881No Hit
TGAGATGAAGCACTGTAGCTCATCTCGTAT3812682.569971717827529No Hit
TAGCTTATCAGACTGATGTTGAATCTCGTA3746002.5250254558425897No Hit
TACAGTACTGTGATAACTGAATCTCGTATG3701202.4948276073584066No Hit
TACCCTGTAGAACCGAATTTGTATCTCGTA3273032.2062157148255395No Hit
TCGTACCGTGAGTAATAATGCGATCTCGTA2565531.7293188888755573No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGT2194431.4791755502041253No Hit
GTACAGTACTGTGATAACTGAATCTCGTAT1810431.2203368489111317No Hit
TGTAAACATCCTCGACTGGAAGATCTCGTA1504101.0138523193093536No Hit
TCAGTGCACTACAGAACTTTGTATCTCGTA1355340.9135792849230366No Hit
TAATACTGCCGGGTAATGATGGATCTCGTA1353470.9123187943724692No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTA1336100.9006103874936688No Hit
TAATACTGCCGGGTAATGATGGAATCTCGT1080590.7283815422661355No Hit
TACCCTGTAGATCCGAATTTGTATCTCGTA797420.5375082218360911No Hit
TAGCACCATCTGAAATCGGTTATCTCGTAT790600.5329111386516687No Hit
CACCCGTAGAACCGACCTTGCGATCTCGTA771320.519915278857583No Hit
CACCCGTAGAACCGACCTTGCATCTCGTAT739650.49856782659209053No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTA733230.49424036705484836No Hit
TGAGATGAAGCACTGTAGCTCAATCTCGTA700520.4721918933066873No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTA600590.40483316564989336No Hit
TGAGATGAAGCACTGTAGCTAATCTCGTAT598780.40361311864640304No Hit
CTTTCAGTCGGATGTTTGCAGCATCTCGTA560980.37813368398787395No Hit
TGAGATGAAGCACTGTAGCATCTCGTATGC542910.36595343572650835No Hit
TGTAAACATCCTCGACTGGAAGCATCTCGT510950.3444105063168102No Hit
TACAGTACTGTGATAACTGAATATCTCGTA500050.3370632619311497No Hit
TAGCTTATCAGACTGATGTTGATCTCGTAT494380.3332413467323703No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTAT444550.2996529808848967No Hit
TGAGATGAAGCACTGTAGCTCTTATCTCGT441800.2977993183105328No Hit
TATTGCACTTGTCCCGGCCTGTATCTCGTA429510.28951513175092114No Hit
TGAGATGAAGCACTGTAGCTCTAATCTCGT413740.27888521946084166No Hit
CTTTCAGTCGGATGTTTACAGATCTCGTAT400390.26988653023620246No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTA397320.2678171687440944No Hit
TAGCTTATCAGACTGATGTTGACATCTCGT376380.2537023708142109No Hit
TGAGATGAAGCACTGTAGATCTCGTATGCC367560.2477571693938874No Hit
CGTACCGTGAGTAATAATGCGATCTCGTAT356520.24031555673171384No Hit
TGAGGTAGTAGGTTGTATAGATCTCGTATG353800.23848211593088842No Hit
TGAGATGAAGCACTGTAGCAATCTCGTATG352820.23782153799529696No Hit
GCGGGTGATGCGAACTGGAGTCTGAGCATC348630.23499723029108435No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC334450.2254390719985462No Hit
CTTTCAGTCGGATGTTTGCAGATCTCGTAT323960.2183681918512454No Hit
TGTGACAGATTGATAACTGAATCTCGTATG323460.21803116229227015No Hit
CTTATCAGATTGTATTGTAATTATCTCGTA308700.20808204971132072No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTA307680.20739450941101117No Hit
AGCAGCATTGTACAGGGCTATGATCTCGTA302750.2040713979595152No Hit
TTTCAGTCGGATGTTTGCAGCATCTCGTAT251460.16949890579983384No Hit
TACCCTGTAGAACCGAATTTGATCTCGTAT243500.16413339522094783No Hit
TTCTCGTATGCCGTCTTCTGCTTGAAAAAA231580.1560986105349778TruSeq Adapter, Index 20 (100% over 24bp)
AAGCTGCCAGTTGAAGACCTGTATCTCGTA224590.1513869373005038No Hit
CTTTCAGTCGGATGTTTACAATCTCGTATG215770.14544173588018033No Hit
TGAGATGAAGCACTGTAGAATCTCGTATGC210490.14188270373740167No Hit
TCAGTGCACTACAGAACTTTGATCTCGTAT207090.13959090273636995No Hit
TACCCTGTAGAACCGAATTTGTGATCTCGT199540.13450175639584364No Hit
ACCCTGTAGAACCGAATTTGTATCTCGTAT195350.13167744869163103No Hit
TACAGTACTGTGATAACTGAAATCTCGTAT194860.1313471597238353No Hit
CTAGACTGAAGCTCCTTGAGGATCTCGTAT194390.13103035193839854No Hit
GTTTGTGATGACTTACATGGAATCTCGTAT193320.1303091086821915No Hit
GGAGAGAACGCGGTCTGAGTGGATCTCGTA191340.12897447162864953No Hit
ACTGCAGTGAAGGCACTTGTAGATCTCGTA183060.12339326213201933No Hit
TACCGTACTGTGATAACTGAATCTCGTATG177480.11963201225385552No Hit
TGAGGTAGTAGATTGTATAGTATCTCGTAT176460.118944471953546No Hit
TAATACTGCCGGGTAATGATGATCTCGTAT175970.11861418298575026No Hit
TACAGTACTGTGATAACTGAAGATCTCGTA175020.11797382682369728No Hit
GTCTACGGCCATACCACCCTGAACGCGCCC170040.11461701241630376No Hit
GTGAAATGTTTAGGACCACTAGATCTCGTA164440.11084228135578095No Hit
CTTTCAGTCGGATGTTTGCATCTCGTATGC161320.10873921690777537No Hit
TGAGGTAGTAGGTTGTGTGGATCTCGTATG158010.1065080812273592No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAA150610.10152004375452546Illumina Paired End PCR Primer 2 (100% over 23bp)
ACCCTGTAGATCCGAATTTGTATCTCGTAT150320.10132456661031983No Hit
ACCCTGTAGAACCGAATTTGTGATCTCGTA149930.10106168355431913No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)