FastQCFastQC Report
Wed 8 May 2024
EGAF00000828781

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000828781
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18460987
Total Bases553.8 Mbp
Sequences flagged as poor quality0
Sequence length30
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTATGCCGTCTTCTGCTTGAAAAAA11874866.432407974719878Illumina Paired End PCR Primer 2 (100% over 24bp)
TGAGATGAAGCACTGTAGCTATCTCGTATG8956174.851403665470324No Hit
TGTAAACATCCTCGACTGGAAGATCTCGTA7449314.035163450361566No Hit
AAGCTGCCAGTTGAAGAACTGTATCTCGTA7176493.8873815359926316No Hit
TGAGATGAAGCACTGTAGCTCTATCTCGTA6918053.7473890209662137No Hit
TCAGTGCACTACAGAACTTTGTATCTCGTA3833202.0763786898284473No Hit
TAGCTTATCAGACTGATGTTGAATCTCGTA3199761.7332551071077618No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGT2874791.5572244322581452No Hit
TCAGTGCACTACAGAACTTTGATCTCGTAT1867271.0114681300626018No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTA1857441.0061433876747758No Hit
TCAGTGCACTACAGAACTTTGAATCTCGTA1842780.998202317135048No Hit
TGTAAACATCCTCGACTGGAAGCATCTCGT1837760.9954830692421809No Hit
TGAGATGAAGCACTGTAGCTCATCTCGTAT1772270.9600082595800539No Hit
TAGCACCATCTGAAATCGGTTATCTCGTAT1745860.9457024155859056No Hit
AACCCGTAGATCCGAACTTGTATCTCGTAT1671800.9055853839234056No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTAT1646140.8916858020646459No Hit
TACAGTACTGTGATAACTGAATCTCGTATG1637620.887070664206632No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTA1517680.8221012235152975No Hit
CACCCGTAGAACCGACCTTGCGATCTCGTA1305680.7072644599121379No Hit
CACCCGTAGAACCGACCTTGCATCTCGTAT1268530.6871409421392258No Hit
TGAGGTAGTAGGTTGTATAGATCTCGTATG1254940.6797794722459856No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTA1200330.6501981719612282No Hit
AACCCGTAGATCCGAACTTGTGAATCTCGT1042600.5647585364747832No Hit
GTACAGTACTGTGATAACTGAATCTCGTAT966580.5235798064318012No Hit
TAATACTGCCGGGTAATGATGGAATCTCGT957840.5188454983474069No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTA945530.512177382498563No Hit
TGAGGTAGTAGATTGTATAGTATCTCGTAT854420.4628246582915638No Hit
TACCCTGTAGAACCGAATTTGTATCTCGTA819650.4439903456949512No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC806680.4369647191669655No Hit
TAATACTGCCGGGTAATGATGGATCTCGTA803290.43512841431500926No Hit
CGCGACCTCAGATCAGACGATCTCGTATGC692790.3752724597010983No Hit
CTTTCAGTCGGATGTTTGCAGATCTCGTAT649260.3516930053631477No Hit
CTTTCAGTCGGATGTTTGCAGCATCTCGTA623750.33787467593146564No Hit
TAGCTTATCAGACTGATGTTGATCTCGTAT605290.3278752105724358No Hit
TGAGGTAGTAGGTTGTATAGAATCTCGTAT561530.3041711691796327No Hit
TGTAAACATCCTTGACTGGATCTCGTATGC548280.29699387145443523No Hit
AGCAGCATTGTACAGGGCTATGATCTCGTA548120.2969072021988857No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTA545640.2955638287378676No Hit
TATTGCACTTGTCCCGGCCTGTATCTCGTA524720.28423182357476334No Hit
AAACCGTTACCATTACTGAGTATCTCGTAT510330.2764370074037753No Hit
CGCGACCTCAGATCAGACGTGGATCTCGTA494040.26761299382313636No Hit
TGAGATGAAGCACTGTAGCTAATCTCGTAT489740.26528375758024203No Hit
TGAGATGAAGCACTGTAGCTCAATCTCGTA473330.2563947420579409No Hit
TCGTACCGTGAGTAATAATGCGATCTCGTA470350.25478052717333044No Hit
TGTAAACATCCTCGACTGGAAATCTCGTAT469280.2542009265268428No Hit
TACAGTACTGTGATAACTGAATATCTCGTA440280.2384921239584861No Hit
TTTGGCAATGGTAGAACTCACAATCTCGTA439200.23790710648352661No Hit
TACCCTGTAGATCCGAATTTGTATCTCGTA372540.20179852789019354No Hit
TAGCACCATTTGAAATCGGTTATCTCGTAT364600.19749756608354688No Hit
TTCAAGTAATCCAGGATAGGATCTCGTATG354560.19205907029781236No Hit
TGAGATGAAGCACTGTAGCATCTCGTATGC332990.18037497128403807No Hit
TGAGGTAGTAGGTTGTGTGGATCTCGTATG323470.17521815057883958No Hit
TTTCAGTCGGATGTTTGCAGCATCTCGTAT323180.175061062553156No Hit
TGAGGTAGTAGGTTGTATGGTTATCTCGTA321720.17427020559626633No Hit
TACAGTACTGTGATAACTGAAGATCTCGTA316840.17162679330200492No Hit
TGAGGTAGTAGATTGTATAGATCTCGTATG307210.16641038748361614No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCA305170.16530535447535932No Hit
ACTGGTGCGGACCAGGGGATCTCGTATGCC300940.16301403603176798No Hit
CTTTCAGTCGGATGTTTACAGTATCTCGTA300740.16290569946233102No Hit
AAGCTGCCAGTTGAAGACCTGTATCTCGTA294300.15941726192646147No Hit
TGTAAACATCCTCGACTGGAAGAATCTCGT284030.15385417908587445No Hit
TTCTCGTATGCCGTCTTCTGCTTGAAAAAA282340.15293873507413228TruSeq Adapter, Index 20 (100% over 24bp)
TTCAAGTAATCCAGGATAGGCTATCTCGTA275790.1493907124250724No Hit
TGTCAACATCCTCGACTGGAAGATCTCGTA273680.14824776161751266No Hit
TGAGATGAAGCACTGTAGCAATCTCGTATG267030.1446455706837343No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGT265260.14368679204421736No Hit
TAGCACCATCTGAAATCGGTTAATCTCGTA263920.14296093702898985No Hit
CTTTCAGTCGGATGTTTACAGATCTCGTAT258190.13985709431462143No Hit
AACCCGTAGATCCGAACTTGTGATCTCGTA251640.13630907166556155No Hit
TGTAAACATCCTTGACTGGAAGATCTCGTA251310.1361303163259906No Hit
TGGCTCAGTTCAGCAGGAACAGTATCTCGT237660.12873634546191923No Hit
TGAGATGAAGCACTGTAGCTCTAATCTCGT236990.12837341795430549No Hit
TGAGGTAGTAGTTTGTGCTGTAAATCTCGT228010.12350910598658675No Hit
TAGCACCATCTGAAATCGGTATCTCGTATG227030.12297825679634573No Hit
TGAGGTAGTAGTTTGTGCTGTATCTCGTAT222460.12050276618471158No Hit
TGAGATGAAGCACTGTAGCTCTTATCTCGT222010.12025900890347845No Hit
AAACCGTTACCATTACTGAGTTATCTCGTA214760.11633180826138927No Hit
TACAGTACTGTGATAACTGAAATCTCGTAT211010.1143004975844466No Hit
TGGCTCAGTTCAGCAGGAACAGATCTCGTA203340.11014579014653983No Hit
AACCCGTAGATCCGATCTTGTATCTCGTAT202830.10986953189447562No Hit
TCAGTGCATCACAGAACTTTGAATCTCGTA201210.1089920056820364No Hit
TCCCTGAGACCCTAACTTGTGAATCTCGTA198690.10762696490713092No Hit
TAGCACCATTTGAAATCGGTATCTCGTATG192600.104328116367776No Hit
TGAGATGAAGCACTGTAGATCTCGTATGCC192080.10404644128723996No Hit
AAGCTGCCAGTTGAAGAACTGTAATCTCGT188630.10217763546445269No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)