FastQCFastQC Report
Thu 9 May 2024
EGAF00000828782

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000828782
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16563704
Total Bases496.9 Mbp
Sequences flagged as poor quality0
Sequence length30
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCTGCCAGTTGAAGAACTGTATCTCGTA15597539.416692063562595No Hit
TGAGATGAAGCACTGTAGCTATCTCGTATG12072137.288303389145326No Hit
ATCTCGTATGCCGTCTTCTGCTTGAAAAAA11668857.04483127686899Illumina Paired End PCR Primer 2 (100% over 24bp)
TGAGATGAAGCACTGTAGCTCTATCTCGTA8296845.0090487007012445No Hit
TAGCTTATCAGACTGATGTTGAATCTCGTA8282525.000403291437712No Hit
TGTAAACATCCTCGACTGGAAGATCTCGTA2694541.626773818223267No Hit
TGAGATGAAGCACTGTAGCTCATCTCGTAT2252821.3600943363875617No Hit
AACCCGTAGATCCGAACTTGTATCTCGTAT2022761.2212002822557082No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGT1996791.205521422020099No Hit
TCAGTGCACTACAGAACTTTGTATCTCGTA1720811.0389041001940147No Hit
TACCCTGTAGAACCGAATTTGTATCTCGTA1627560.9826063059325377No Hit
TAGCTTATCAGACTGATGTTGATCTCGTAT1349010.8144373987847163No Hit
CACCCGTAGAACCGACCTTGCATCTCGTAT1294040.7812503773310606No Hit
TACAGTACTGTGATAACTGAATCTCGTATG1272190.7680588834478085No Hit
CACCCGTAGAACCGACCTTGCGATCTCGTA1220570.7368943564796859No Hit
TGTAAACATCCTCGACTGGAAGCATCTCGT1217680.7351495776548531No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTA1196010.7220667551170922No Hit
AACCCGTAGATCCGAACTTGTGAATCTCGT1086190.655765159773442No Hit
TAGCACCATCTGAAATCGGTTATCTCGTAT980590.5920113037518662No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTA842440.5086060460872762No Hit
TACCCTGTAGATCCGAATTTGTATCTCGTA807970.4877954834256879No Hit
AAGCTGCCAGTTGAAGACCTGTATCTCGTA781260.4716698632141699No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTA674300.4070949348044374No Hit
TAGCTTATCAGACTGATGTTGACATCTCGT664380.4011059362084713No Hit
GTACAGTACTGTGATAACTGAATCTCGTAT615420.3715473302348315No Hit
TCAGTGCACTACAGAACTTTGATCTCGTAT606120.3659326440511132No Hit
CTTTCAGTCGGATGTTTGCAGCATCTCGTA605860.3657756743298479No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTAT593580.3583618736485511No Hit
TGAGATGAAGCACTGTAGCTAATCTCGTAT582770.35183555562209995No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTA533020.3218000031876928No Hit
TGAGATGAAGCACTGTAGCTCAATCTCGTA519280.3135047571485219No Hit
TATTGCACTTGTCCCGGCCTGTATCTCGTA493610.2980070158220649No Hit
TAATACTGCCGGGTAATGATGGAATCTCGT489440.2954894629848493No Hit
TGAGATGAAGCACTGTAGCATCTCGTATGC434770.26248356044034593No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTA434440.26228432964027854No Hit
TGAGGTAGTAGGTTGTATAGATCTCGTATG411270.24829591255675662No Hit
CGCGACCTCAGATCAGACGTGGATCTCGTA393490.23756159854100267No Hit
AAGCTGCCAGTTGAAGAACTGTAATCTCGT383460.23150618967834732No Hit
TTTGGCAATGGTAGAACTCACAATCTCGTA378390.22844528011367504No Hit
TGTAAACATCCTTGACTGGATCTCGTATGC364720.22019229515330632No Hit
TACAGTACTGTGATAACTGAATATCTCGTA362120.21862259794065386No Hit
TCAGTGCACTACAGAACTTTGAATCTCGTA361930.21850788929819082No Hit
TGAGATGAAGCACTGTAGCAATCTCGTATG358670.2165397304854035No Hit
TGAGATGAAGCACTGTAGATCTCGTATGCC350670.2117098929080114No Hit
CTTTCAGTCGGATGTTTACAGTATCTCGTA338420.2043142041176297No Hit
TAATACTGCCGGGTAATGATGGATCTCGTA337400.20369839982651225No Hit
TGAGGTAGTAGATTGTATAGTATCTCGTAT297600.17966995787898649No Hit
CTTTCAGTCGGATGTTTACAGATCTCGTAT278540.1681628698508498No Hit
TTCTCGTATGCCGTCTTCTGCTTGAAAAAA272250.16436541005562524TruSeq Adapter, Index 20 (100% over 24bp)
CTTTCAGTCGGATGTTTGCAGATCTCGTAT268800.1622825426003749No Hit
TATGGCACTGGTAGAATTCACTATCTCGTA262510.15848508280515036No Hit
TGAGATGAAGCACTGTAGCTCTTATCTCGT261790.15805039742318505No Hit
CGCGACCTCAGATCAGACGATCTCGTATGC261390.15780890554431545No Hit
TGAGATGAAGCACTGTAGCTCTAATCTCGT260900.15751307799270017No Hit
TTTGGCAATGGTAGAACTCACACTATCTCG253970.15332923119128428No Hit
AACCCGTAGATCCGAACTTGTGATCTCGTA252430.1523994874576363No Hit
AGCAGCATTGTACAGGGCTATGATCTCGTA246400.14875899738367698No Hit
TCGTACCGTGAGTAATAATGCGATCTCGTA245650.14830620011079648No Hit
TGAGATGAAGCACTGTAGAATCTCGTATGC230600.13922006816832758No Hit
TTTGGCAATGGTAGAACTCACATCTCGTAT220720.13325521876024832No Hit
TGAGATGAAGCACTGTAGAAAATCTCGTAT216530.1307255913290892No Hit
TACAGTACTGTGATAACTGAAGATCTCGTA201800.12183265288971597No Hit
TGTAAACATCCCCGACTGGAAGCATCTCGT189160.11420150951743643No Hit
AAGATGCCAGTTGAAGAACTGTATCTCGTA184610.11145453939529469No Hit
TAGCACCATCTGAAATCGGTTAATCTCGTA181150.10936563464307258No Hit
TGTAAACATCCTTGACTGGAAGATCTCGTA180690.10908791898237254No Hit
ACCCTGTAGATCCGAATTTGTATCTCGTAT177930.10742162501817226No Hit
TTTGGCAATGGTAGAACTCACACATCTCGT172030.10385961980484558No Hit
AACCCGTAGATCCGATCTTGTATCTCGTAT171180.10334644956224767No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)