FastQCFastQC Report
Wed 8 May 2024
EGAF00000828783

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000828783
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20994870
Total Bases629.8 Mbp
Sequences flagged as poor quality0
Sequence length30
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTATGCCGTCTTCTGCTTGAAAAAA14081006.706876489351922Illumina Paired End PCR Primer 2 (100% over 24bp)
TGAGATGAAGCACTGTAGCTATCTCGTATG10883145.18371392630676No Hit
TGAGATGAAGCACTGTAGCTCTATCTCGTA8849314.214986803919244No Hit
AAGCTGCCAGTTGAAGAACTGTATCTCGTA7846863.737513021037996No Hit
TGTAAACATCCTCGACTGGAAGATCTCGTA7828843.7289299719407643No Hit
TAGCTTATCAGACTGATGTTGAATCTCGTA4446072.1176935127485903No Hit
TCAGTGCACTACAGAACTTTGTATCTCGTA4121101.9629080818314186No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGT2825061.3455953763943287No Hit
AACCCGTAGATCCGAACTTGTATCTCGTAT2621581.248676462392956No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTA2469341.17616351041945No Hit
TGAGATGAAGCACTGTAGCTCATCTCGTAT2330281.1099282824804344No Hit
TACAGTACTGTGATAACTGAATCTCGTATG2297721.0944197320583553No Hit
TAGCACCATCTGAAATCGGTTATCTCGTAT2260571.0767249332813207No Hit
TCAGTGCACTACAGAACTTTGATCTCGTAT2022150.9631638585997436No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTAT2001810.9534757776542555No Hit
TGTAAACATCCTCGACTGGAAGCATCTCGT1854110.8831252586941476No Hit
TCAGTGCACTACAGAACTTTGAATCTCGTA1834800.873927773784739No Hit
TGAGGTAGTAGGTTGTATAGATCTCGTATG1552200.7393234633031783No Hit
TACCCTGTAGAACCGAATTTGTATCTCGTA1461220.6959890678056115No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTA1392000.6630191089537587No Hit
AACCCGTAGATCCGAACTTGTGAATCTCGT1342540.6394609730853299No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTA1324330.6307874256901805No Hit
CACCCGTAGAACCGACCTTGCATCTCGTAT1249250.5950263088078183No Hit
GTACAGTACTGTGATAACTGAATCTCGTAT1188970.5663145330264012No Hit
CACCCGTAGAACCGACCTTGCGATCTCGTA1149310.5474242041031928No Hit
TAGCTTATCAGACTGATGTTGATCTCGTAT963790.45905976078918326No Hit
TGAGGTAGTAGATTGTATAGTATCTCGTAT877280.4178544568268344No Hit
TACAGTACTGTGATAACTGAATATCTCGTA775130.3691997140253786No Hit
TAATACTGCCGGGTAATGATGGAATCTCGT762190.36303630363036304No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTA759690.3618455365525007No Hit
CTTTCAGTCGGATGTTTGCAGCATCTCGTA728840.34715147081167924No Hit
CTTTCAGTCGGATGTTTGCAGATCTCGTAT712330.3392876450294762No Hit
TGTAAACATCCTTGACTGGATCTCGTATGC672330.3202353717836786No Hit
TGAGGTAGTAGGTTGTATAGAATCTCGTAT667200.317791917739905No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC619630.29513400178234017No Hit
TATTGCACTTGTCCCGGCCTGTATCTCGTA617200.293976576182658No Hit
TAATACTGCCGGGTAATGATGGATCTCGTA610050.29057098233997164No Hit
TGAGGTAGTAGGTTGTGTGGATCTCGTATG598480.28506011230362466No Hit
TAGCACCATTTGAAATCGGTTATCTCGTAT593380.28263094746478545No Hit
TGAGATGAAGCACTGTAGCTAATCTCGTAT587100.2796397405651952No Hit
TGAGATGAAGCACTGTAGCTCAATCTCGTA577120.2748861983903687No Hit
TGTAAACATCCTCGACTGGAAATCTCGTAT576150.2744241807641581No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTA509950.242892668542363No Hit
AAACCGTTACCATTACTGAGTATCTCGTAT497180.23681023030864204No Hit
AGCAGCATTGTACAGGGCTATGATCTCGTA459490.21885822584278922No Hit
TTTGTTCGTTCGGCTCGCGTGAATCTCGTA452880.21570983768892113No Hit
TACCCTGTAGATCCGAATTTGTATCTCGTA451090.2148572484611717No Hit
TGAGGTAGTAGGTTGTATGGTTATCTCGTA449590.21414278821445426No Hit
CGCGACCTCAGATCAGACGATCTCGTATGC418380.19927725201442067No Hit
TGAGATGAAGCACTGTAGCATCTCGTATGC403210.19205167738595189No Hit
TACAGTACTGTGATAACTGAAGATCTCGTA401120.19105619610885896No Hit
TTCAAGTAATCCAGGATAGGATCTCGTATG390410.18595494994729664No Hit
TCGTACCGTGAGTAATAATGCGATCTCGTA381300.18161579471556624No Hit
TTTGGCAATGGTAGAACTCACAATCTCGTA361080.17198487058981551No Hit
TTCTCGTATGCCGTCTTCTGCTTGAAAAAA356810.1699510404208266TruSeq Adapter, Index 20 (100% over 24bp)
TAGCACCATCTGAAATCGGTTAATCTCGTA353420.16833636026324525No Hit
TGAGGTAGTAGGTTGTGTGGTTATCTCGTA339800.16184906122305115No Hit
AAGCTGCCAGTTGAAGACCTGTATCTCGTA331910.1580910003253176No Hit
AACCCGTAGATCCGAACTTGTGATCTCGTA328920.15666684290019417No Hit
TGAGGTAGTAGATTGTATAGATCTCGTATG322130.15343271951672005No Hit
TTTCAGTCGGATGTTTGCAGCATCTCGTAT318300.15160846435343492No Hit
TGAGATGAAGCACTGTAGATCTCGTATGCC316810.15089876717502895No Hit
TACAGTACTGTGATAACTGAAATCTCGTAT316380.15069395523763662No Hit
TGTCAACATCCTCGACTGGAAGATCTCGTA315980.15050343250517864No Hit
TGTAAACATCCTTGACTGGAAGATCTCGTA310650.1479647170951761No Hit
CTTTCAGTCGGATGTTTACAGTATCTCGTA294260.14015804813271052No Hit
TGAGGTAGTAGGTTGTGTGGTATCTCGTAT292170.13916256685561756No Hit
TGAGATGAAGCACTGTAGCAATCTCGTATG291090.13864815547798107No Hit
CGCGACCTCAGATCAGACGTGGATCTCGTA289160.1377288832938713No Hit
TGAGATGAAGCACTGTAGCTCTTATCTCGT288350.13734307476064392No Hit
ACTGGTGCGGACCAGGGGATCTCGTATGCC287000.13670006053859823No Hit
CTTTCAGTCGGATGTTTACAGATCTCGTAT282630.13461859968649484No Hit
TGAGATGAAGCACTGTAGCTCTAATCTCGT269670.12844566315485642No Hit
AACCCGTAGATCCGATCTTGTATCTCGTAT264670.1260641289991317No Hit
TAGCACCATTTGAAATCGGTATCTCGTATG262650.1251019892002189No Hit
TTCAAGTAATCCAGGATAGGCTATCTCGTA262620.12508769999528457No Hit
TGTAAACATCCTCGACTGGAAGAATCTCGT259330.12352065052081772No Hit
TAGCTTATCAGACTGATGTTGACATCTCGT255980.12192502263648215No Hit
TAGCACCATCTGAAATCGGTATCTCGTATG251120.11961017143711775No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGT238320.11351344399846248No Hit
TAGCACCATTTGAAATCGGATCTCGTATGC235370.1121083388465849No Hit
CTAGCACCATCTGAAATCGGTTATCTCGTA231790.11040316039108602No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAA216070.10291561700548754Illumina Paired End PCR Primer 2 (100% over 23bp)
TCCCTGAGACCCTAACTTGTGAATCTCGTA210090.10006730215524078No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)