FastQCFastQC Report
Wed 8 May 2024
EGAF00000828784

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000828784
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5620771
Total Bases168.6 Mbp
Sequences flagged as poor quality0
Sequence length30
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTATGCCGTCTTCTGCTTGAAAAAA61931411.018310477334872Illumina Paired End PCR Primer 2 (100% over 24bp)
TAGCTTATCAGACTGATGTTGAATCTCGTA5248279.337277750685805No Hit
TAGCTTATCAGACTGATGTTGATCTCGTAT1680352.9895364888553546No Hit
TACAGTACTGTGATAACTGAATCTCGTATG1527802.7181324412611723No Hit
TAGCACCATCTGAAATCGGTTATCTCGTAT1319162.3469378133355723No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGT1092171.9430964186229966No Hit
TCTCCCAACCCTTGTACCAGTGATCTCGTA856421.5236699733897716No Hit
TCTCCCAACCCTTGTACCAGTATCTCGTAT634201.1283149589264534No Hit
TATTGCACTTGTCCCGGCCTGTATCTCGTA623351.1090115573112658No Hit
AAGCTGCCAGTTGAAGAACTGTATCTCGTA614961.0940847794724247No Hit
AACTTGACCGCTCTGACCAATCTCGTATGC565501.0060897339528687No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTA545840.9711123260492199No Hit
TCTCCCAACCCTTGTACCAGATCTCGTATG456970.8130023443403048No Hit
TCAGTGCACTACAGAACTTTGATCTCGTAT405870.7220895496365178No Hit
TACAGTACTGTGATAACTGAATATCTCGTA393070.6993168730766651No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTA386820.6881974021001745No Hit
TGAGGTAGTAGGTTGTATAGATCTCGTATG371970.6617775390600328No Hit
GCGGGTGATGCGAACTGGAGTCTGAGCATC364340.6482028888919331No Hit
GTACAGTACTGTGATAACTGAATCTCGTAT351480.625323465410706No Hit
TTCAAGTAATCCAGGATAGGATCTCGTATG345900.6153960017228953No Hit
GTAGTGTTTCCTACTTTATGGATCTCGTAT335030.5960570178005828No Hit
TCAGTGCACTACAGAACTTTGTATCTCGTA314050.5587311776266992No Hit
TAGCTTATCAGACTGATGTTGACATCTCGT313910.5584821014768259No Hit
TCGTTTCCGGCTCGAAGGACCAATCTCGTA312520.5560091311316543No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTA305140.5428792598026142No Hit
GTTTGTGATGACTTACATGGAATCTCGTAT301810.53695480566634No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTAT286500.5097165495623287No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGT259740.4621074226293866No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTA257690.4584602361490977No Hit
TCAGTGCACTACAGAACTTTGAATCTCGTA247990.44120281719358434No Hit
TAGCACCATCTGAAATCGGTATCTCGTATG240560.4279839900967323No Hit
TACAGTACTGTGATAACTGAAATCTCGTAT232160.41303942110432895No Hit
TGTAAACATCCTTGACTGGAAGATCTCGTA215970.3842355434868277No Hit
GTAGTGTTTCCTACTTTATGGAATCTCGTA215380.383185865426647No Hit
TAGCACCATTTGAAATCGGTTATCTCGTAT208730.371354748307661No Hit
TGTAGTGTTTCCTACTTTATGGATCTCGTA196350.3493293001974284No Hit
TGTAAACATCCTTGACTGGATCTCGTATGC193460.34418765681789915No Hit
AGCAGCATTGTACAGGGCTATGATCTCGTA192660.34276436453290837No Hit
TACAGTACTGTGATAACTGAAGATCTCGTA186630.33203629893479025No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGT171250.3046735047558422No Hit
TAGCACCATCTGAAATCGGTTAATCTCGTA171020.3042643082239073No Hit
TGAGGTAGTAGATTGTATAGTATCTCGTAT169810.30211157864285876No Hit
TAGCAGCACGTAAATATTGGCGATCTCGTA163500.2908853607449939No Hit
CACTAGATTGTGAGCTCCTGGAATCTCGTA150870.2684151337957017No Hit
TGAGGTAGTAGGTTGTATAGAATCTCGTAT148270.2637894338694816No Hit
TTAATGCTAATCGTGATAGGGATCTCGTAT143010.25443128709566715No Hit
TTAATGCTAATCGTGATAGGGGATCTCGTA138150.24578478646434804No Hit
CTTTCAGTCGGATGTTTACAGTATCTCGTA126120.22438202872879895No Hit
TTAATGCTAATCGTGATAGGGGTATCTCGT120950.2151840023370459No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAA118860.2114656512425075Illumina Paired End PCR Primer 2 (100% over 23bp)
CTTTCAGTCGGATGTTTACAGATCTCGTAT118570.21094970778919833No Hit
TAGCACCATTTGAAATCGGTATCTCGTATG116950.20806754091209195No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTA113980.2027835683040636No Hit
TTCAAGTAATCCAGGATAGGCTATCTCGTA108300.19267819308062897No Hit
TCTCCCAACCCTTGTACCAGTGAATCTCGT106020.1886218100684052No Hit
TAGCTTATCAGACTGATGTTGAAATCTCGT103130.18348016668887596No Hit
TGAGGTAGTAGATTGTATAGATCTCGTATG100310.1784630613842834No Hit
CGCGGGTGATGCGAACTGGAGTCTGAGCAT98160.17463796336837065No Hit
ATGTGTGACTGAAAGGTATTTTCTGAGCAT93850.16696997618298273No Hit
TAGCACCATCTGAAATCGGATCTCGTATGC93410.1661871654262378No Hit
CTTTCAGTCGGATGTTTACAATCTCGTATG93380.16613379196555064No Hit
ATCGCGTATGCCGTCTTCTGCTTGAAAAAA86850.15451616868931325Illumina Paired End PCR Primer 2 (95% over 24bp)
TGTAGTGTTTCCTACTTTATGGAATCTCGT83630.1487874172422253No Hit
CTAGCACCATCTGAAATCGGTTATCTCGTA79570.14156420889589702No Hit
TACAGTACTGTGATAACTGAAAATCTCGTA78640.13990963161459521No Hit
TTCTCGTATGCCGTCTTCTGCTTGAAAAAA75420.1341808801675073TruSeq Adapter, Index 20 (100% over 24bp)
TCTCCCAACCCTTGTACCAGTGTATCTCGT74330.1322416444292073No Hit
TAGCTTATCAGACTGATGTTATCTCGTATG71800.12774048257792392No Hit
TAGCACCATTTGAAATCAGTGTTATCTCGT71570.12733128604598906No Hit
TAGCACCATCTGAAATCGGTAATCTCGTAT69440.12354177033720107No Hit
AATGTGTGACTGAAAGGTATTTTCTGAGCA68030.1210332176849048No Hit
TTCAAGTAATTCAGGATAGGTTATCTCGTA67760.1205528565387204No Hit
TAGCACCATTTGAAATCGGATCTCGTATGC65400.11635414429799755No Hit
TACCACAGGGTAGAACCACGGAATCTCGTA64680.11507318124150583No Hit
TCGTTTCCCGGCCAATGCACCAATCTCGTA64180.1141836235633866No Hit
ATAGCGTATGCCGTCTTCTGCTTGAAAAAA63420.11283149589264532Illumina Single End Adapter 1 (95% over 21bp)
TGAGGTAGTAGTTTGTACAGTTATCTCGTA62590.11135483014696738No Hit
TGTAAACATCCTTGACTGGAAGCATCTCGT59000.10496780601807118No Hit
TTAATGCTAATCGTGATAGGGAATCTCGTA58110.10338439335101893No Hit
TAGCACCATTTGAAATCAGTGATCTCGTAT57300.10194330991246574No Hit
TGTCAGTTTGTCAAATACCCCAATCTCGTA57150.10167644260902996No Hit
AAAGACTTTTTCTCTGACCAATCTCGTATG56500.10052001762747495No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)