FastQCFastQC Report
Wed 8 May 2024
EGAF00000828785

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000828785
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5698035
Total Bases170.9 Mbp
Sequences flagged as poor quality0
Sequence length30
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTATGCCGTCTTCTGCTTGAAAAAA5608159.842252636215818Illumina Paired End PCR Primer 2 (100% over 24bp)
TAGCTTATCAGACTGATGTTGAATCTCGTA3648646.403330270874082No Hit
TACAGTACTGTGATAACTGAATCTCGTATG1875333.291187225069695No Hit
TCTCCCAACCCTTGTACCAGTGATCTCGTA1335662.3440712456136192No Hit
TAGCTTATCAGACTGATGTTGATCTCGTAT1309962.2989679775571754No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGT1254412.2014782288982078No Hit
TAGCACCATCTGAAATCGGTTATCTCGTAT1209332.12236323574706No Hit
TCTCCCAACCCTTGTACCAGTATCTCGTAT963111.690249357892677No Hit
TCAGTGCACTACAGAACTTTGAATCTCGTA841911.477544451727657No Hit
TCAGTGCACTACAGAACTTTGTATCTCGTA762131.337531271745435No Hit
TCAGTGCACTACAGAACTTTGATCTCGTAT696251.2219124663151422No Hit
TCTCCCAACCCTTGTACCAGATCTCGTATG623761.0946931705403704No Hit
TATTGCACTTGTCCCGGCCTGTATCTCGTA596051.046062370624259No Hit
AAGCTGCCAGTTGAAGAACTGTATCTCGTA594701.0436931328080645No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTA518930.9107174666354279No Hit
TACAGTACTGTGATAACTGAATATCTCGTA478080.8390260853083562No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTA450870.7912727808797244No Hit
TGAGGTAGTAGGTTGTATAGATCTCGTATG422450.7413959373713921No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTA390380.6851133768044598No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTAT377530.6625617427762378No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGT366400.643028693224945No Hit
GCGGGTGATGCGAACTGGAGTCTGAGCATC348010.6107544091954507No Hit
GTACAGTACTGTGATAACTGAATCTCGTAT342780.6015758063964156No Hit
TTCAAGTAATCCAGGATAGGATCTCGTATG332120.5828676026033536No Hit
GTTTGTGATGACTTACATGGAATCTCGTAT295890.5192842795805922No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTA291640.511825567937017No Hit
TGTAAACATCCTTGACTGGATCTCGTATGC245430.43072743498416566No Hit
TACAGTACTGTGATAACTGAAATCTCGTAT241490.4238127705428275No Hit
TGAGGTAGTAGGTTGTATAGAATCTCGTAT239910.4210398848023924No Hit
GTAGTGTTTCCTACTTTATGGATCTCGTAT226080.3967683596187106No Hit
TACAGTACTGTGATAACTGAAGATCTCGTA213030.3738657273954969No Hit
TAGCTTATCAGACTGATGTTGACATCTCGT200900.3525776868692453No Hit
TAGCACCATCTGAAATCGGTTAATCTCGTA193530.33964340338379806No Hit
TAGCACCATTTGAAATCGGTTATCTCGTAT187270.3286571598805553No Hit
TGTAAACATCCTTGACTGGAAGATCTCGTA172650.302999191826656No Hit
TGAGGTAGTAGATTGTATAGTATCTCGTAT170620.29943656014748943No Hit
TAGCACCATCTGAAATCGGTATCTCGTATG167530.2940136380348664No Hit
AGCAGCATTGTACAGGGCTATGATCTCGTA160560.2817813509394028No Hit
TCGTTTCCGGCTCGAAGGACCAATCTCGTA157360.2761653798195343No Hit
TCTCCCAACCCTTGTACCAGTGAATCTCGT153210.26888216727345476No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTA149720.2627572487708482No Hit
CTTTCAGTCGGATGTTTACAGTATCTCGTA138150.24245200319057358No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGT136540.23962646772088975No Hit
CTTTCAGTCGGATGTTTACAGATCTCGTAT129110.22658688477694505No Hit
TCTCCCAACCCTTGTACCAGTGTATCTCGT126570.22212920770054942No Hit
CACTAGATTGTGAGCTCCTGGAATCTCGTA125350.21998811871109955No Hit
TTAATGCTAATCGTGATAGGGGATCTCGTA118730.20837007845687155No Hit
TTCAAGTAATCCAGGATAGGCTATCTCGTA109020.1913291160900205No Hit
CTAGCACCATCTGAAATCGGTTATCTCGTA103760.18209786356173663No Hit
GTAGTGTTTCCTACTTTATGGAATCTCGTA103430.18151871654000018No Hit
TACAGTACTGTGATAACTGAAAATCTCGTA102350.17962332628704455No Hit
TAGCACCATCTGAAATCGGATCTCGTATGC101940.1789037799873114No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAA99590.17477955119615798Illumina Paired End PCR Primer 2 (100% over 23bp)
TGTAGTGTTTCCTACTTTATGGATCTCGTA96140.16872483233254973No Hit
TTAATGCTAATCGTGATAGGGGTATCTCGT93950.16488140209738972No Hit
TTAATGCTAATCGTGATAGGGATCTCGTAT93890.1647761026388922No Hit
TACCACAGGGTAGAACCACGGAATCTCGTA93510.16410920606840781No Hit
TAGCTTATCAGACTGATGTTGAAATCTCGT90300.1584756850387897No Hit
CGCGGGTGATGCGAACTGGAGTCTGAGCAT89390.15687864325157708No Hit
TGAGGTAGTAGATTGTATAGATCTCGTATG88070.1545620551646313No Hit
TAGCACCATTTGAAATCGGTATCTCGTATG87730.15396535823314528No Hit
TTCTCGTATGCCGTCTTCTGCTTGAAAAAA84300.14794573918903622TruSeq Adapter, Index 20 (100% over 24bp)
ATGTGTGACTGAAAGGTATTTTCTGAGCAT83890.14722619288930308No Hit
ATCGCGTATGCCGTCTTCTGCTTGAAAAAA79650.13978503115547727Illumina Paired End PCR Primer 2 (95% over 24bp)
TAGCACCATTTGAAATCGGATCTCGTATGC77750.13645054830305536No Hit
CTTTCAGTCGGATGTTTACAATCTCGTATG73670.129290185125223No Hit
TGTAAACATCCTTGACTGGAAGCATCTCGT70720.1241129617490942No Hit
TAGCACCATCTGAAATCGGTAATCTCGTAT65370.11472376003306405No Hit
ATGCAGTGTGGAACACAATGAACTGAACAT63400.11126642781239499No Hit
TTAATGCTAATCGTGATAGGGAATCTCGTA63360.11119622817339662No Hit
TAGCACCATCTGAAATCGGTTAAATCTCGT62920.1104240321444147No Hit
CGCGACCTCAGATCAGACGTGGCATCTCGT58450.10257922248634838No Hit
ATAGCGTATGCCGTCTTCTGCTTGAAAAAA57770.1013858286233763Illumina Single End Adapter 1 (95% over 21bp)
TTAATGCTAATCGTGATAGGGGAATCTCGT57170.10033283403840096No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)