FastQCFastQC Report
Fri 10 May 2024
EGAF00000828786

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000828786
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42539301
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length30
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTTGGTTATCTAGCTGTATGAATCTCGT934107321.958689448141143No Hit
TCTTTGGTTATCTAGCTGTATGATCTCGTA658539015.480719817187405No Hit
ATCTCGTATGCCGTCTTCTGCTTGAAAAAA11921082.802368567363154Illumina Paired End PCR Primer 2 (100% over 24bp)
AGCAGCATTGTACAGGGCTATGAATCTCGT7820231.838354137506867No Hit
AAGCTGCCAGTTGAAGAACTGTATCTCGTA7810061.8359634071091109No Hit
TCTTTGGTTATCTAGCTGTATGTATCTCGT6318431.485315896469479No Hit
CTTTGGTTATCTAGCTGTATGAATCTCGTA5835661.3718279009803194No Hit
TACCCTGTAGAACCGAATTTGTATCTCGTA5707811.3417733403752918No Hit
TCTTTGGTTATCTAGCTGTATATCTCGTAT4412331.0372361313600333No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTA4351351.0229011520429072No Hit
TAGCACCATCTGAAATCGGTTATCTCGTAT3978900.9353468219893881No Hit
TGTAAACATCCTCGACTGGAAGATCTCGTA2941720.6915299336959015No Hit
TTTGGCAATGGTAGAACTCACAATCTCGTA2567120.6034701886615391No Hit
TGAGATGAAGCACTGTAGCTATCTCGTATG2350530.552554918568126No Hit
TGAGATGAAGCACTGTAGCTCTATCTCGTA2320270.5454414965586765No Hit
TACAGTACTGTGATAACTGAATCTCGTATG2285140.5371832508484331No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTAT2132530.5013081902779738No Hit
CACCCGTAGAACCGACCTTGCGATCTCGTA1981480.46579984941454494No Hit
TATTGCACTTGTCCCGGCCTGTATCTCGTA1973460.46391453399763194No Hit
CTTTGGTTATCTAGCTGTATGATCTCGTAT1687010.3965768031778425No Hit
CACCCGTAGAACCGACCTTGCATCTCGTAT1673840.3934808425742586No Hit
AACCCGTAGATCCGAACTTGTATCTCGTAT1519200.3571285762311891No Hit
TTTGGCAATGGTAGAACTCACACTATCTCG1487130.34958966533089014No Hit
TGAGGTAGTAGGTTGTATGGTTATCTCGTA1461720.3436163654875288No Hit
TGAGGTAGTAGGTTGTGTGGTTATCTCGTA1440600.33865154483850124No Hit
TTTGGCAATGGTAGAACTCACACATCTCGT1376250.3235243569234953No Hit
TTTGGCAATGGTAGAACTCACATCTCGTAT1342700.3156375324549879No Hit
TCTGTGGTTATCTAGCTGTATGAATCTCGT1340060.3150169298738595No Hit
TGTAAACATCCTCGACTGGAAGCATCTCGT1257560.2956230992135954No Hit
AACCCGTAGATCCGAACTTGTGAATCTCGT1253380.2946404784601421No Hit
TAGCTTATCAGACTGATGTTGAATCTCGTA1220160.28683122931427574No Hit
TGAGGTAGTAGGTTGTATAGATCTCGTATG1136210.26709653738786165No Hit
ACCACTGACCGTTGACTGTACCATCTCGTA1076430.2530436501530667No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTA1060150.24921660090277462No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTA1049340.2466754213944418No Hit
AGCAGCATTGTACAGGGCTATGATCTCGTA957400.22506246635317306No Hit
GTACAGTACTGTGATAACTGAATCTCGTAT954080.22428201159205696No Hit
TGGTAGACTATGGAACGTAGGATCTCGTAT952170.22383301502767994No Hit
TCTTTGGTTATCTAGCTGTATGATCTCGGA930670.2187788652192475No Hit
TCTGTGGTTATCTAGCTGTATGATCTCGTA926160.21771866914315308No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTA923810.2171662388152546No Hit
TATGGCACTGGTAGAATTCACTATCTCGTA913230.2146791269560353No Hit
TGAGGTAGTAGGTTGTGTGGATCTCGTATG881120.2071308129863253No Hit
TCACAGTGAACCGGTCTCTTTATCTCGTAT866100.20359996042248082No Hit
AACATTCAACGCTGTCGGTGAGATCTCGTA804980.18923207036241615No Hit
TCCCTGAGACCCTAACTTGTGAATCTCGTA775690.18234667278618424No Hit
TAGCACCATCTGAAATCGGTTAATCTCGTA762130.17915903225584265No Hit
AACATTCAACGCTGTCGGTGAGAATCTCGT705810.16591951052510243No Hit
TACAGTACTGTGATAACTGAATATCTCGTA688750.16190910142129508No Hit
TGAGGTAGTAGGTTGTGTGGTATCTCGTAT658820.1548732547344866No Hit
AACATTCAACGCTGTCGGTGAGTATCTCGT657810.1546358272318579No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTA647060.15210875232764168No Hit
TTTGGTTATCTAGCTGTATGAATCTCGTAT641310.1507570610998051No Hit
AACCCGTAGATCCGATCTTGTATCTCGTAT602280.14158201612198565No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGT580070.1363609618315073No Hit
CTTTCAGTCGGATGTTTGCAGCATCTCGTA573850.13489878453809104No Hit
AGAATTGTGGCTGGACATCTGTATCTCGTA512700.12052384217596805No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGT476530.11202111666103774No Hit
TGAGGTAGTAGATTGTATAGTATCTCGTAT463600.10898157447392001No Hit
TGGTAGACTATGGAACGTAGATCTCGTATG453820.10668252400292144No Hit
AAGCTGCCAGTTGAAGACCTGTATCTCGTA453370.10657673947204727No Hit
ACCACTGACCGTTGACTGTACATCTCGTAT452510.10637457347970998No Hit
TACAGTACTGTGATAACTGAAGATCTCGTA433070.10180468174594594No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)