FastQCFastQC Report
Wed 8 May 2024
EGAF00000828787

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000828787
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32528573
Total Bases975.8 Mbp
Sequences flagged as poor quality0
Sequence length30
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTTGGTTATCTAGCTGTATGAATCTCGT891820727.41653315071645No Hit
TCTTTGGTTATCTAGCTGTATGATCTCGTA602642218.52654895128661No Hit
ATCTCGTATGCCGTCTTCTGCTTGAAAAAA9786273.008515006176262Illumina Paired End PCR Primer 2 (100% over 24bp)
CTTTGGTTATCTAGCTGTATGAATCTCGTA5160231.5863683906453565No Hit
TCTTTGGTTATCTAGCTGTATGTATCTCGT4681381.4391593507652487No Hit
TCTTTGGTTATCTAGCTGTATATCTCGTAT4128071.2690596664046714No Hit
TGAGATGAAGCACTGTAGCTATCTCGTATG4089331.2571501368965678No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGT3241100.9963855469466798No Hit
TACAGTACTGTGATAACTGAATCTCGTATG2955190.9084905138629967No Hit
AAGCTGCCAGTTGAAGAACTGTATCTCGTA2893170.8894241994568899No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTA2809090.8635761550314549No Hit
TGTAAACATCCTCGACTGGAAGATCTCGTA2276250.6997693996598007No Hit
TGAGATGAAGCACTGTAGCTCTATCTCGTA2202710.6771615834484962No Hit
CTTTGGTTATCTAGCTGTATGATCTCGTAT1573790.48381771927099293No Hit
TAGCTTATCAGACTGATGTTGAATCTCGTA1525410.46894464137729003No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTAT1306640.4016899235020239No Hit
TCTGTGGTTATCTAGCTGTATGAATCTCGT1277890.39285154009061507No Hit
TAGCACCATCTGAAATCGGTTATCTCGTAT1151100.3538735006912231No Hit
TGTAAACATCCTCGACTGGAAGCATCTCGT1032050.31727490781719814No Hit
TCTTTGGTTATCTAGCTGTATGATCTCGGA1030610.31683222009154843No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTA925740.28459287162704616No Hit
TCTGTGGTTATCTAGCTGTATGATCTCGTA863160.2653544008831866No Hit
GTACAGTACTGTGATAACTGAATCTCGTAT815150.25059506914121316No Hit
AACCCGTAGATCCGAACTTGTATCTCGTAT764080.23489502598223413No Hit
GGGCCAGTGGCGCAATGGAATCTCGTATGC743050.22842994065555844No Hit
CTTTCAGTCGGATGTTTGCAGCATCTCGTA730680.2246271301234149No Hit
TGAGGTAGTAGGTTGTATAGATCTCGTATG613350.18855730314391597No Hit
TGAGGTAGTAGGTTGTGTGGTTATCTCGTA600990.18475756683208944No Hit
TGAGATGAAGCACTGTAGCTCATCTCGTAT581260.17869213014662524No Hit
TATTGCACTTGTCCCGGCCTGTATCTCGTA580800.1785507160120427No Hit
CACCCGTAGAACCGACCTTGCATCTCGTAT573280.17623890233364986No Hit
CACCCGTAGAACCGACCTTGCGATCTCGTA551320.16948791451749207No Hit
TTTGGTTATCTAGCTGTATGAATCTCGTAT545830.1678001675634526No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTA490520.1507966549900606No Hit
AACCCGTAGATCCGAACTTGTGAATCTCGT485740.149327177678529No Hit
AGAATTGTGGCTGGACATCTGTATCTCGTA480460.14770398935114676No Hit
TACAGTACTGTGATAACTGAATATCTCGTA462370.14214272479767248No Hit
TCTTTGGTTATCTAGCTGTATGATTTCGGA447910.1376974022192735No Hit
TCTTTGGTTATCTAGCTGTATGAATCTCGA446960.1374053512891574No Hit
AACATTCAACGCTGTCGGTGAGATCTCGTA441670.13577908874145816No Hit
TCAGTGGTTATCTAGCTGTATGATCTCGTA434120.13345805240211428No Hit
AGCAGCATTGTACAGGGCTATGATCTCGTA416960.12818269033812213No Hit
TGAGGTAGTAGATTGTATAGTATCTCGTAT396720.12196046841649033No Hit
TGAGGTAGTAGGTTGTGTGGATCTCGTATG393610.12100438589789968No Hit
TGAGGTAGTAGGTTGTATGGTTATCTCGTA393270.12089986240712126No Hit
TCTTTGGTTATCTAGCTGTATGATTTCGTA385920.11864031047411763No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGC384530.118212993850053No Hit
AACCCGTAGATCCGATCTTGTATCTCGTAT383450.11788097805581572No Hit
ACCACTGACCGTTGACTGTACCATCTCGTA368320.11322968271617695No Hit
GGCCGCGTGGCCTAATGGAATCTCGTATGC366490.1126671003981638No Hit
TCCTTGGTTATCTAGCTGTATGAATCTCGT366150.1125625769073854No Hit
TCGTACCGTGAGTAATAATGCGATCTCGTA363600.1117786507265474No Hit
TACAGTACTGTGATAACTGAAGATCTCGTA351970.10820333249786272No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTA344860.10601756185246737No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGT339960.10451119389713161No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCG331350.10186429020418448No Hit
CGCGACCTCAGATCAGACGTGGATCTCGTA331200.1018181768994293No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTA326680.10042862931614No Hit
TCTTTGGTTATCTAGCTGTATGAATTTCGT326660.10042248087550598No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)