FastQCFastQC Report
Fri 10 May 2024
EGAF00000828788

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000828788
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18260578
Total Bases547.8 Mbp
Sequences flagged as poor quality0
Sequence length30
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTATGCCGTCTTCTGCTTGAAAAAA16942239.278035996450933Illumina Paired End PCR Primer 2 (100% over 24bp)
TCTTTGGTTATCTAGCTGTATGAATCTCGT16150698.844566694438697No Hit
TCTTTGGTTATCTAGCTGTATGATCTCGTA11724526.4206729929359305No Hit
AAGCTGCCAGTTGAAGAACTGTATCTCGTA5698733.1207829237387776No Hit
TCCAGCATCAGTGATTTTGTTGATCTCGTA5003602.7401104170963264No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGT3606221.9748662939365884No Hit
TAGCTTATCAGACTGATGTTGAATCTCGTA3318211.8171440137327526No Hit
TCCAGCATCAGTGATTTTGTTGAATCTCGT3292421.8030206929923027No Hit
AGAATTGTGGCTGGACATCTGTATCTCGTA2430761.3311517302464357No Hit
AACCCGTAGATCCGAACTTGTATCTCGTAT2021051.1067831478280699No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTA1898051.0394249294847075No Hit
TAGCACCATCTGAAATCGGTTATCTCGTAT1807900.9900562840891456No Hit
AACCCGTAGATCCGAACTTGTGAATCTCGT1681740.9209675619249293No Hit
CTTTGGTTATCTAGCTGTATGAATCTCGTA1598070.8751475446177005No Hit
AGAATTGTGGCTGGACATCTGAATCTCGTA1507730.8256748499417708No Hit
TACAGTACTGTGATAACTGAATCTCGTATG1476100.8083533828994898No Hit
CACCCGTAGAACCGACCTTGCGATCTCGTA1309550.7171459742402458No Hit
TGTAAACATCCTCGACTGGAAGATCTCGTA1178760.6455217354018038No Hit
GTACAGTACTGTGATAACTGAATCTCGTAT1105380.6053368080681784No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTAT1099980.6023796179945673No Hit
CACCCGTAGAACCGACCTTGCATCTCGTAT1077160.5898827517946037No Hit
TGAGATGAAGCACTGTAGCTATCTCGTATG1036610.5676764448529504No Hit
AACATTCAACGCTGTCGGTGAGATCTCGTA1020040.5586022523492958No Hit
TGAGATGAAGCACTGTAGCTCTATCTCGTA935670.5123988955880805No Hit
TGAGGTAGTAGGTTGTGTGGTTATCTCGTA909140.4978703302819878No Hit
TCTTTGGTTATCTAGCTGTATGTATCTCGT908000.497246034599781No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTA746190.40863438167181787No Hit
AACATTCAACGCTGTCGGTGAGTATCTCGT725770.39745182217123687No Hit
AGAATTGTGGCTGGACATCTGATCTCGTAT704280.38568330093384773No Hit
TCTTTGGTTATCTAGCTGTATATCTCGTAT690510.3781424662461396No Hit
TGAGGTAGTAGGTTGTGTGGATCTCGTATG626870.3432914336008422No Hit
TTCACAGTGGCTAAGTTCTGCATCTCGTAT575130.3149571716733172No Hit
TGAGGTAGTAGGTTGTATAGATCTCGTATG570750.31255856194694387No Hit
TAGCACCATTTGAAATCGGTTATCTCGTAT558460.3058282163905217No Hit
CGCGACCTCAGATCAGACGTGGCATCTCGT552340.3024767343070959No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTA544000.2979095185267411No Hit
AGCAGCATTGTACAGGGCTATGATCTCGTA532670.29170489565007196No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGT529460.2899470104396476No Hit
TGAGGTAGTAGGTTGTGTGGTATCTCGTAT491430.2691207255323462No Hit
TAGCACCATCTGAAATCGGTTAATCTCGTA491330.2690659627532053No Hit
AACCCGTAGATCCGAACTTGTGATCTCGTA482030.26397302429309744No Hit
TATTGCACTTGTCCCGGCCTGTATCTCGTA473870.2595043815151963No Hit
TTCAAGTAATCCAGGATAGGCTATCTCGTA470200.25749458752072363No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAA468450.25653623888575705Illumina Paired End PCR Primer 2 (100% over 23bp)
TAGCACCATTTGAAATCAGTGTTATCTCGT420640.23035415417847122No Hit
AACATTCAACGCTGTCGGTGAGAATCTCGT413440.2264112340803232No Hit
TGAGGTAGTAGGTTGTATGGTTATCTCGTA398930.21846515482697204No Hit
TAGCTTATCAGACTGATGTTGATCTCGTAT392530.2149603369619516No Hit
TACAGTACTGTGATAACTGAATATCTCGTA379680.20792331984234017No Hit
TGGCTCAGTTCAGCAGGAACAGTATCTCGT364880.19981842852948026No Hit
TACAGTACTGTGATAACTGAAGATCTCGTA361730.19809340098654052No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTA361190.1977976819791794No Hit
TGAGGTAGTAGATTGTATAGTATCTCGTAT360900.19763886991967067No Hit
CTTTCAGTCGGATGTTTGCAGCATCTCGTA328940.18013668570622463No Hit
TGGCTCAGTTCAGCAGGAACAGATCTCGTA328430.17985739553260582No Hit
TCCCTGAGACCCTAACTTGTGAATCTCGTA327290.17923309985039904No Hit
TCCAGCATCAGTGATTTTGTTATCTCGTAT326620.1788661892301547No Hit
AAGCTGCCAGTTGAAGACCTGTATCTCGTA323680.17725616352341092No Hit
TGATTGTCCAAACGCAATTCTATCTCGTAT312170.17095296764428816No Hit
CTTTGGTTATCTAGCTGTATGATCTCGTAT302060.16541645067313862No Hit
TACAGTACTGTGATAACTGAAATCTCGTAT294840.16146257801916236No Hit
AAACCGTTACCATTACTGAGTATCTCGTAT294130.16107376228726167No Hit
TGTAAACATCCTCGACTGGAAGCATCTCGT292430.1601427950418656No Hit
TAGCTTATCAGACTGATGTTGACATCTCGT287930.15767846998052307No Hit
TTCTCGTATGCCGTCTTCTGCTTGAAAAAA268640.14711472988423477TruSeq Adapter, Index 20 (100% over 24bp)
TCCAGCATCAGTGATTTTGTATCTCGTATG238110.13039565341250425No Hit
TGAGATGAAGCACTGTAGCTCATCTCGTAT233250.1277341823462543No Hit
TAGCACCATCTGAAATCGGTATCTCGTATG233100.1276520381775429No Hit
TCTGTGGTTATCTAGCTGTATGAATCTCGT225890.12370364180148076No Hit
CTAGACTGAAGCTCCTTGAGGATCTCGTAT221320.12120098279473958No Hit
AGCATTGTGGCTGGACATCTGTATCTCGTA215880.11822188760947216No Hit
AAACCGTTACCATTACTGAGTTATCTCGTA213730.11704448785794185No Hit
ATCGCGTATGCCGTCTTCTGCTTGAAAAAA210340.1151880296450638Illumina Paired End PCR Primer 2 (95% over 24bp)
TCGAGGAGCTCACAGTCTAGTATCTCGTAT209060.1144870660720597No Hit
CGCGACCTCAGATCAGACGTGGATCTCGTA205470.1125210823008998No Hit
TTATGGTTTGCCTGGGACTGAATCTCGTAT202930.1111301077107198No Hit
TGATTGTCCAAACGCAATTCTTGATCTCGT202290.11077962592421772No Hit
ACCATCGACCGTTGATTGTACCATCTCGTA200940.11004032840581499No Hit
TTTGGTTATCTAGCTGTATGAATCTCGTAT200200.10963508384017198No Hit
TGATTGTCCAAACGCAATTCTTATCTCGTA191120.10466262349417418No Hit
TGTAAACATCCTTGACTGGATCTCGTATGC189980.1040383278119674No Hit
ATCACATTGCCAGGGATTACCATCTCGTAT188490.10322236240276732No Hit
TAGCACCATTTGAAATCGGTTAATCTCGTA188270.10310188428865724No Hit
TCGTTTCCGGCTCGAAGGACCAATCTCGTA185640.10166162319725039No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)