FastQCFastQC Report
Wed 8 May 2024
EGAF00000828789

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000828789
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4288850
Total Bases128.6 Mbp
Sequences flagged as poor quality0
Sequence length30
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTATGCCGTCTTCTGCTTGAAAAAA44466710.367977429847162Illumina Paired End PCR Primer 2 (100% over 24bp)
TCTCCCAACCCTTGTACCAGTGATCTCGTA2775136.470569033657041No Hit
TCTCCCAACCCTTGTACCAGTATCTCGTAT1650573.848514170465276No Hit
TAGCTTATCAGACTGATGTTGAATCTCGTA1258742.9349126222647093No Hit
TCTCCCAACCCTTGTACCAGATCTCGTATG1203052.805064294624433No Hit
TACAGTACTGTGATAACTGAATCTCGTATG1062312.4769110600743787No Hit
TAGCTTATCAGACTGATGTTGATCTCGTAT752761.755155810998286No Hit
TAGCACCATCTGAAATCGGTTATCTCGTAT570281.3296804504704058No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGT515021.200834722594635No Hit
TGAGGTAGTAGGTTGTATAGATCTCGTATG461081.0750667428331604No Hit
TATTGCACTTGTCCCGGCCTGTATCTCGTA388550.9059538104620118No Hit
TCTCCCAACCCTTGTACCAGTGAATCTCGT382080.8908681814472411No Hit
TTCAAGTAATCCAGGATAGGATCTCGTATG345860.8064166384928361No Hit
GCGGGTGATGCGAACTGGAGTCTGAGCATC339780.7922403441481982No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTA312140.7277941639367196No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTA307270.7164391386968535No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTAT293200.6836331417512853No Hit
TCAGTGCACTACAGAACTTTGAATCTCGTA280150.6532054047122189No Hit
TCAGTGCACTACAGAACTTTGATCTCGTAT272280.6348554973944065No Hit
AAGCTGCCAGTTGAAGAACTGTATCTCGTA264110.6158061018687994No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGT254110.592489828275645No Hit
TGAGGTAGTAGGTTGTATAGAATCTCGTAT252210.5880597362929457No Hit
GTTTGTGATGACTTACATGGAATCTCGTAT242860.5662590204833464No Hit
TGTAAACATCCTTGACTGGATCTCGTATGC229260.5345488883966565No Hit
TACAGTACTGTGATAACTGAATATCTCGTA204660.4771908553574968No Hit
GTACAGTACTGTGATAACTGAATCTCGTAT202650.4725042843652727No Hit
CACTAGATTGTGAGCTCCTGGAATCTCGTA168800.39357869825244524No Hit
TACAGTACTGTGATAACTGAAATCTCGTAT156970.3659955465917437No Hit
TCAGTGCACTACAGAACTTTGTATCTCGTA156700.3653660072047285No Hit
TCTCCCAACCCTTGTACCAGTGTATCTCGT152230.35494363290858855No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTA146110.34067407346957806No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTA141220.32927241568252563No Hit
TGAGGTAGTAGGTTGTGTGGATCTCGTATG133390.31101577345908576No Hit
CTTTCAGTCGGATGTTTACAGTATCTCGTA130230.303647831003649No Hit
TGTAAACATCCTTGACTGGAAGATCTCGTA129980.30306492416382014No Hit
GTAGTGTTTCCTACTTTATGGATCTCGTAT129400.3017125802954172No Hit
TAGCACCATCTGAAATCGGTATCTCGTATG123660.2883290392529466No Hit
TACAGTACTGTGATAACTGAAGATCTCGTA122400.28539118878020914No Hit
CTTTCAGTCGGATGTTTACAGATCTCGTAT119590.2788393159005328No Hit
TAGCACCATTTGAAATCGGTTATCTCGTAT116940.2726605033983469No Hit
AGCAGCATTGTACAGGGCTATGATCTCGTA104540.2437483241428355No Hit
TAGCACCATCTGAAATCGGATCTCGTATGC102720.2395047623488814No Hit
TGAGGTAGTAGATTGTATAGTATCTCGTAT100740.23488814017743684No Hit
CTTTCAGTCGGATGTTTACAATCTCGTATG98120.22877927649603041No Hit
AACTTGACCGCTCTGACCAATCTCGTATGC98050.22861606258087835No Hit
TAGCACCATTTGAAATCGGATCTCGTATGC96820.22574816092892033No Hit
TGTAGTGTTTCCTACTTTATGGATCTCGTA88460.2062557562050433No Hit
ATGTGTGACTGAAAGGTATTTTCTGAGCAT85870.20021684134441634No Hit
CGCGGGTGATGCGAACTGGAGTCTGAGCAT84910.19797847907947352No Hit
TAGCACCATTTGAAATCGGTATCTCGTATG79760.18597059817899902No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAA77610.18095759935647085Illumina Paired End PCR Primer 2 (100% over 23bp)
TAGCTTATCAGACTGATGTTGACATCTCGT75420.17585133543957004No Hit
TAGCACCATCTGAAATCGGTTAATCTCGTA73100.17044195996595823No Hit
TAGCACCATTTGAAATCAGTGATCTCGTAT72330.16864660689928535No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTA71540.16680462128542617No Hit
TGAGGTAGTAGGTTGTGTGGTTATCTCGTA71440.16657145854949462No Hit
TCGTTTCCGGCTCGAAGGACCAATCTCGTA71410.16650150972871516No Hit
TGAGGTAGTAGATTGTATAGATCTCGTATG71330.16631497953996993No Hit
TACAGTACTGTGATAACTGAAAATCTCGTA70950.16542896114343006No Hit
TGAGGTAGTAGGTTGTGTGGTATCTCGTAT67960.1584573953390769No Hit
TACCACAGGGTAGAACCACGGAATCTCGTA67680.1578045396784686No Hit
TTAATGCTAATCGTGATAGGGGATCTCGTA66690.1554962285927463No Hit
GTAGTGTTTCCTACTTTATGGAATCTCGTA65180.15197547128018002No Hit
TTCAAGTAATCCAGGATAGGCTATCTCGTA63420.14787180712778483No Hit
CGCGACCTCAGATCAGACGTGGCATCTCGT60870.14192615736153047No Hit
TCTCCCAACCCTTGTACCAGAATCTCGTAT58730.13693647481259544No Hit
ATCGCGTATGCCGTCTTCTGCTTGAAAAAA58220.13574734485934456Illumina Paired End PCR Primer 2 (95% over 24bp)
TAGCACCATCTGAAATCGGTAATCTCGTAT57770.13469811254765265No Hit
CTAGCACCATCTGAAATCGGTTATCTCGTA56050.1306877134896301No Hit
TTCTCGTATGCCGTCTTCTGCTTGAAAAAA53750.1253249705632046TruSeq Adapter, Index 20 (100% over 24bp)
AATGTGTGACTGAAAGGTATTTTCTGAGCA51730.12061508329738742No Hit
TGAGGTAGTAGTTTGTGCTGTAAATCTCGT51420.11989227881599963No Hit
TTAATGCTAATCGTGATAGGGATCTCGTAT51330.11968243235366124No Hit
TGAGGTAGTAGGTTGTGTGAATCTCGTATG49740.11597514485234969No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGT49660.11578861466360446No Hit
TCTTTGGTTATCTAGCTGTATGAATCTCGT48550.11320050829476433No Hit
CACTAGATTGTGAGCTCCTGGAAATCTCGT47550.1108688809354489No Hit
TTAATGCTAATCGTGATAGGGGTATCTCGT46810.10914347668955547No Hit
ATCTCGTATGCCGTCTTCTGCTTGCAAAAA44870.10462011961248355Illumina Paired End PCR Primer 2 (100% over 24bp)
TCTCCCAACCCTTGTACCAGTAATCTCGTA43920.10240507362113388No Hit
TAGCTTATCAGACTGATGTTGAAATCTCGT43580.10161232031896664No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)