FastQCFastQC Report
Wed 8 May 2024
EGAF00000828790

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000828790
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25392540
Total Bases761.7 Mbp
Sequences flagged as poor quality0
Sequence length30
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCTTATCAGACTGATGTTGAATCTCGTA407638316.0534668843684No Hit
ATCTCGTATGCCGTCTTCTGCTTGAAAAAA15809156.22590335586751Illumina Paired End PCR Primer 2 (100% over 24bp)
TCTTTGGTTATCTAGCTGTATGATCTCGTA11109904.375261395669751No Hit
AAGCTGCCAGTTGAAGAACTGTATCTCGTA10879124.284376434968696No Hit
TCTTTGGTTATCTAGCTGTATGAATCTCGT10199984.016919930026694No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGT5948322.3425462754021456No Hit
TAGCTTATCAGACTGATGTTGACATCTCGT4614491.8172620777598458No Hit
TAGCTTATCAGACTGATGTTGATCTCGTAT3978111.5666451642884092No Hit
TGAGATGAAGCACTGTAGCTATCTCGTATG3837131.5111249209413473No Hit
TGTAAACATCCTCGACTGGAAGATCTCGTA3649191.4371110570269852No Hit
TGAGATGAAGCACTGTAGCTCTATCTCGTA2377960.9364797692550646No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTA2311030.9101216341492423No Hit
AACCCGTAGATCCGAACTTGTATCTCGTAT2132430.8397860158928566No Hit
CACCCGTAGAACCGACCTTGCGATCTCGTA1987140.7825684236393838No Hit
CACCCGTAGAACCGACCTTGCATCTCGTAT1761110.6935540910834442No Hit
TAGCACCATCTGAAATCGGTTATCTCGTAT1467070.5777563016539503No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTAT1421900.5599676125350201No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTA1397270.5502679133320258No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTA1396470.5499528601707431No Hit
AACCCGTAGATCCGAACTTGTGAATCTCGT1356260.5341175006517662No Hit
TACCCTGTAGAACCGAATTTGTATCTCGTA1205930.4749150734822117No Hit
TGTAAACATCCTCGACTGGAAGCATCTCGT1149990.45288498117951176No Hit
TCAGTGCACTACAGAACTTTGTATCTCGTA1024840.40359885226133346No Hit
CTTTGGTTATCTAGCTGTATGAATCTCGTA989920.38984678177133913No Hit
TGAGGTAGTAGGTTGTATGGTTATCTCGTA963620.37948940909416706No Hit
TCTTTGGTTATCTAGCTGTATGTATCTCGT954440.3758741740684469No Hit
TCTTTGGTTATCTAGCTGTATATCTCGTAT939960.37017171184922815No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGT911520.35897157196562457No Hit
TACAGTACTGTGATAACTGAATCTCGTATG856280.3372171511790471No Hit
CGCGACCTCAGATCAGACGTGGATCTCGTA855310.33683514922099167No Hit
TGAGGTAGTAGGTTGTGTGGATCTCGTATG789480.3109102122119331No Hit
GTACAGTACTGTGATAACTGAATCTCGTAT762910.30044650909282805No Hit
TGAGATGAAGCACTGTAGCTCATCTCGTAT760760.2995998037218805No Hit
TGAGGTAGTAGGTTGTATAGATCTCGTATG713050.28081082081587744No Hit
AGCAGCATTGTACAGGGCTATGATCTCGTA698870.27522650353213973No Hit
AAGCTGCCAGTTGAAGACCTGTATCTCGTA627640.24717495768442227No Hit
CTTTCAGTCGGATGTTTGCAGCATCTCGTA621930.2449262657457663No Hit
TATTGCACTTGTCCCGGCCTGTATCTCGTA601400.23684121399434638No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTA594860.23426565440085945No Hit
TGAGGTAGTAGATTGTATAGTATCTCGTAT587270.23127658753318886No Hit
TGAGGTAGTAGGTTGTGTGGTTATCTCGTA569330.22421152039142203No Hit
AACCCGTAGATCCGAACTTGTGATCTCGTA552670.2176505383077077No Hit
TGAGGTAGTAGGTTGTGTGGTATCTCGTAT394980.1555496220543514No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTA383600.15106799083510355No Hit
CGCGACCTCAGATCAGACGTATCTCGTATG360400.1419314491579023No Hit
CTTTCAGTCGGATGTTTGCAGATCTCGTAT350940.13820594552573315No Hit
TTCACAGTGGCTAAGTTCTGCATCTCGTAT349520.1376467261644562No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAA327190.12885280480014996Illumina Paired End PCR Primer 2 (100% over 23bp)
TAGCACCATCTGAAATCGGTTAATCTCGTA319780.125934624893768No Hit
CACTAGATTGTGAGCTCCTGGAATCTCGTA314510.12385921219381757No Hit
TAGCTTATCAGACTGATGTTGAAATCTCGT312220.12295737251964553No Hit
TGGCTCAGTTCAGCAGGAACAGATCTCGTA304320.11984622255197785No Hit
CTTTGGTTATCTAGCTGTATGATCTCGTAT299300.11786926396492828No Hit
TTCAAGTAATCCAGGATAGGCTATCTCGTA298060.11738093156493995No Hit
AACCCGTAGATCCGATCTTGTATCTCGTAT287550.11324192065858713No Hit
TGAGGTAGTAGGTTGTATGGTATCTCGTAT282900.11141067415863083No Hit
TACAGTACTGTGATAACTGAAGATCTCGTA281270.11076875334251714No Hit
TTCTCGTATGCCGTCTTCTGCTTGAAAAAA273470.10769698502000981TruSeq Adapter, Index 20 (100% over 24bp)
TCGTACCGTGAGTAATAATGCGATCTCGTA269340.10607052307488735No Hit
TGGCTCAGTTCAGCAGGAACAGTATCTCGT269220.10602326510069493No Hit
TCCCTGAGACCCTAACTTGTGAATCTCGTA265650.10461734036847042No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)