FastQCFastQC Report
Wed 8 May 2024
EGAF00000828791

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000828791
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27394087
Total Bases821.8 Mbp
Sequences flagged as poor quality0
Sequence length30
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTATGCCGTCTTCTGCTTGAAAAAA427920615.620911184227456Illumina Paired End PCR Primer 2 (100% over 24bp)
TACAGTACTGTGATAACTGAATCTCGTATG13662774.987488723387642No Hit
TAGCTTATCAGACTGATGTTGAATCTCGTA12195854.452000900778332No Hit
TCAGTGCACTACAGAACTTTGATCTCGTAT6138562.2408339434710856No Hit
GTTTGTGATGACTTACATGGAATCTCGTAT5874882.1445795948592847No Hit
TCTCCCAACCCTTGTACCAGTGATCTCGTA5696292.0793866939241306No Hit
TCAGTGCACTACAGAACTTTGAATCTCGTA5275231.9256819911537844No Hit
TAGCACCATCTGAAATCGGTTATCTCGTAT4230411.5442785152868939No Hit
TAGCTTATCAGACTGATGTTGATCTCGTAT4216001.5390182560199945No Hit
TACAGTACTGTGATAACTGAATATCTCGTA3714641.356000658098224No Hit
GTAGTGTTTCCTACTTTATGGATCTCGTAT2868281.0470434732867717No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGT2837801.0359169845667788No Hit
TCTCCCAACCCTTGTACCAGTATCTCGTAT2703210.9867859439885696No Hit
TCTCCCAACCCTTGTACCAGATCTCGTATG2541950.9279192257803665No Hit
TATTGCACTTGTCCCGGCCTGTATCTCGTA2409610.8796095303340462No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTA2231410.8145589958884193No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGT2190680.79969082378982No Hit
TCAGTGCACTACAGAACTTTGTATCTCGTA1975930.7212979939795037No Hit
GCGGGTGATGCGAACTGGAGTCTGAGCATC1758470.6419158995881118No Hit
GTACAGTACTGTGATAACTGAATCTCGTAT1734070.6330088679356243No Hit
TACAGTACTGTGATAACTGAAATCTCGTAT1510170.551275901255625No Hit
CTTTCAGTCGGATGTTTACAGTATCTCGTA1506920.5500895138428961No Hit
TAGCACCATTTGAAATCGGTTATCTCGTAT1495700.5459937394518751No Hit
TGTAAACATCCTTGACTGGATCTCGTATGC1458490.5324105161818314No Hit
GTAGTGTTTCCTACTTTATGGAATCTCGTA1194030.43587143459097577No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTA1091560.3984655520733361No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTA1082630.39520572450543795No Hit
AAGCTGCCAGTTGAAGAACTGTATCTCGTA985100.3596031508551462No Hit
CTTTCAGTCGGATGTTTACAGATCTCGTAT962250.3512619347379601No Hit
TGAGGTAGTAGGTTGTATAGATCTCGTATG947340.3458191543306408No Hit
TGTAAACATCCTTGACTGGAAGATCTCGTA929690.3393761580738208No Hit
TGTAGTGTTTCCTACTTTATGGATCTCGTA924080.33732827087831035No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAA872580.318528593415068Illumina Paired End PCR Primer 2 (100% over 23bp)
TGAGGTAGTAGGTTGTATAGTATCTCGTAT813560.2969837979999114No Hit
TCTCCCAACCCTTGTACCAGTGAATCTCGT767190.28005678743737655No Hit
CTTTCAGTCGGATGTTTACAATCTCGTATG762290.2782680802612622No Hit
TAGCACCATCTGAAATCGGTTAATCTCGTA761440.2779577943225485No Hit
TTCAAGTAATCCAGGATAGGATCTCGTATG720930.26316993152573404No Hit
TAGCTTATCAGACTGATGTTGACATCTCGT713570.26048322033875415No Hit
TGAGGTAGTAGGTTGTATAGAATCTCGTAT665270.2428516781741987No Hit
TACAGTACTGTGATAACTGAAAATCTCGTA636930.23250637993520282No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTA635010.23180549875599066No Hit
TCTCCCAACCCTTGTACCAGTGTATCTCGT628820.22954588703759318No Hit
TGTAAACATCCTTGACTGGAAGCATCTCGT603590.22033587029200863No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGT559620.2042849612034889No Hit
TAGCACCATTTGAAATCGGTATCTCGTATG552100.2015398432515747No Hit
CACTAGATTGTGAGCTCCTGGAATCTCGTA547260.19977303861231074No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTA544730.19884948164178642No Hit
TAGCACCATCTGAAATCGGTATCTCGTATG522340.1906761849737865No Hit
ATGTGTGACTGAAAGGTATTTTCTGAGCAT520110.18986214068751406No Hit
TATTGCACTTGTCCCGGCCTGTAAATCTCG470030.17158082326306404No Hit
TTAATGCTAATCGTGATAGGGGTATCTCGT452290.1651049732009685No Hit
TCGTTTCCGGCTCGAAGGACCAATCTCGTA450180.16433473398839685No Hit
CTAGCACCATCTGAAATCGGTTATCTCGTA448840.1638455773320717No Hit
TGTTTGTGATGACTTACATGGAATCTCGTA439100.16029006551669345No Hit
TTAATGCTAATCGTGATAGGGATCTCGTAT431910.1576654115174563No Hit
TTAATGCTAATCGTGATAGGGGATCTCGTA425650.15538024683940005No Hit
ATGCAGTGTGGAACACAATGAACTGAACAT417980.15258037254535986No Hit
TAGCTTATCAGACTGATGTTGAAATCTCGT358500.13086765768101707No Hit
ATCGCGTATGCCGTCTTCTGCTTGAAAAAA351430.1282868087554807Illumina Paired End PCR Primer 2 (95% over 24bp)
TAGCACCATTTGAAATCGGATCTCGTATGC345430.12609655507044276No Hit
TGTCGCTGATGCCATCACCGCAGCGCTCTG343700.12546503192459016No Hit
CGCGGGTGATGCGAACTGGAGTCTGAGCAT343200.12528251078417033No Hit
TACAGTACTGTGATAACTGAAGATCTCGTA330140.1205150585964044No Hit
CCTACTGTGGAATCTGAAGACAATCTCGTA317890.1160432906561186No Hit
TAGCACCATTTGAAATCAGTGATCTCGTAT304000.11097285337525577No Hit
TGAGGTAGTAGATTGTATAGTATCTCGTAT301530.11007119894158181No Hit
TTAATGCTAATCGTGATAGGGAATCTCGTA297040.10843215910061176No Hit
AATGTGTGACTGAAAGGTATTTTCTGAGCA283580.10351869000050998No Hit
TAGCACCATTTGAAATCGGTTAATCTCGTA279710.10210597637366049No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)