FastQCFastQC Report
Wed 8 May 2024
EGAF00000828792

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000828792
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20521524
Total Bases615.6 Mbp
Sequences flagged as poor quality0
Sequence length30
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTATGCCGTCTTCTGCTTGAAAAAA399779019.480960575832476Illumina Paired End PCR Primer 2 (100% over 24bp)
TAGCTTATCAGACTGATGTTGAATCTCGTA10609395.169884069038927No Hit
TAGCTTATCAGACTGATGTTGACATCTCGT7575113.6912999248983653No Hit
AAGCTGCCAGTTGAAGAACTGTATCTCGTA7045543.433244041719319No Hit
CACCCGTAGAACCGACCTTGCGATCTCGTA6259103.0500171429763205No Hit
TATTGCACTTGTCCCGGCCTGTATCTCGTA5242732.5547469086603902No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGT4261722.0767073634492252No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTA3744241.824542855589088No Hit
TCCTTCATTCCACCGGAGTCTGATCTCGTA3181991.550562229198962No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGT2040190.9941708032990142No Hit
AACCCGTAGATCCGAACTTGTATCTCGTAT1530340.7457243428899335No Hit
CACCCGTAGAACCGACCTTGCATCTCGTAT1453910.7084805202576573No Hit
TAATACTGCCGGGTAATGATGGAATCTCGT1354340.6599607319612325No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTA1227790.598293771944033No Hit
GTTTGTGATGACTTACATGGAATCTCGTAT1227010.5979136832137808No Hit
TCCTTCATTCCACCGGAGTCTGTATCTCGT1213790.59147166652925No Hit
AACCCGTAGATCCGAACTTGTGAATCTCGT1176830.5734613082342228No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTA1091310.5317879900147766No Hit
CAACACCAGTCGATGGGCTGTCATCTCGTA1054820.5140066595443886No Hit
TCAGTGCACTACAGAACTTTGTATCTCGTA1045140.5092896609433101No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGT981480.4782685730358038No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAA890610.4339882359614227Illumina Paired End PCR Primer 2 (100% over 23bp)
TCGTTTCCGGCTCGAAGGACCAATCTCGTA835140.40695807972156456No Hit
TTTGGCAATGGTAGAACTCACACTATCTCG698270.34026225342718214No Hit
TCCTTCATTCCACCGGAGTCTATCTCGTAT552470.2692148984646559No Hit
TGAGGTAGTAGGTTGTGTGGTTATCTCGTA506930.24702356413685453No Hit
ATGGCACTGGTAGAATTCACTGATCTCGTA468760.22842358101669252No Hit
TCGTTTCCCGGCCCATGCACCAATCTCGTA466090.22712250805544462No Hit
TATGGCACTGGTAGAATTCACTGATCTCGT461400.2248371027414923No Hit
TAGCTTATCAGACTGATGTTGATCTCGTAT456700.22254682449510088No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTAT442290.21552492885031346No Hit
TCCTTCATTCCACCGGAGTCTGAATCTCGT398670.19426919755082517No Hit
AACCCGTAGATCCGAACTTGTGATCTCGTA393460.19173039975003806No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTA354620.17280393015645426No Hit
CTTTCAGTCGGATGTTTACAGTATCTCGTA352320.1716831556954542No Hit
ATCACATTGCCAGGGATTTCCAATCTCGTA349180.17015305490956714No Hit
TAGCACCATCTGAAATCGGTTAATCTCGTA343280.16727802477048No Hit
TTTGGCAATGGTAGAACTCACACTGATCTC331920.1617423735196275No Hit
TGTTTGTGATGACTTACATGGAATCTCGTA326870.15928154263786645No Hit
ATCGCGTATGCCGTCTTCTGCTTGAAAAAA326150.15893069150224903Illumina Paired End PCR Primer 2 (95% over 24bp)
ACTCGGCGTGGCGTCGGTCGTGGATCTCGT317390.1546620026855705No Hit
AGCAGCATTGTACAGGGCTATGATCTCGTA310620.151363027424279No Hit
CAAAGTGCTTACAGTGCAGGTAGATCTCGT308180.15017403190913112No Hit
CACCCGTAGAACCGACCTTGCGAATCTCGT308060.15011555671986154No Hit
TGTAAACATCCTTGACTGGAAGCATCTCGT296050.14426316486046553No Hit
TAGCTTATCAGACTGATGTTGACTATCTCG295000.14375150695435682No Hit
TAGCACCATCTGAAATCGGTTATCTCGTAT293530.14303518588580458No Hit
TGAGGTAGGAGGTTGTATAGTTATCTCGTA292120.14234810241188714No Hit
GTGAAATGTTTAGGACCACTAGATCTCGTA263910.12860156000109932No Hit
TGTAAACATCCTTGACTGGAAGCTATCTCG258260.12584835317299045No Hit
TGGCTCAGTTCAGCAGGAACAGATCTCGTA253410.12348498094001206No Hit
TCGTTTCCCGGCCAATGCACCAATCTCGTA252790.12318285912878595No Hit
TAATACTGCCGGGTAATGATGGATCTCGTA252510.12304641702049028No Hit
TATGGCACTGGTAGAATTCACTATCTCGTA246860.12029321019238143No Hit
TTATGGTTTGCCTGGGACTGAATCTCGTAT246450.12009341996237707No Hit
CACCCGTAGAACCGACCTTGCGTATCTCGT245090.11943070115065528No Hit
AACCCGTAGATCCGAACTTGTGTATCTCGT238510.11622431160570725No Hit
GTACAGTACTGTGATAACTGAATCTCGTAT231590.11285224235782877No Hit
AACTCGTATGCCGTCTTCTGCTTGAAAAAA229470.11181918068073307Illumina Single End Adapter 1 (95% over 24bp)
ATCACATTGCCAGGGATTTCCATCTCGTAT228760.1114732024775548No Hit
TACAGTACTGTGATAACTGAATCTCGTATG224040.10917317836628508No Hit
TGTAAACATCCTCGACTGGAAGCTATCTCG222560.10845198436529374No Hit
TAACACTGTCTGGTAAAGATGGCATCTCGT222300.10832528812187633No Hit
ACTGGACTTGGAGTCAGAAGGCATCTCGTA218310.10638098807866316No Hit
TAAAGTGCTTATAGTGCAGGTAGATCTCGT216770.10563055648303701No Hit
TTCACAGTGGCTAAGTTCCGCATCTCGTAT211130.10288222258736729No Hit
TGTAAACATCCTCGACTGGAAGCATCTCGT206830.10078686163854107No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)