FastQCFastQC Report
Fri 10 May 2024
EGAF00000828793

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000828793
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20454937
Total Bases613.6 Mbp
Sequences flagged as poor quality0
Sequence length30
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTATGCCGTCTTCTGCTTGAAAAAA414758520.276694081238187Illumina Paired End PCR Primer 2 (100% over 24bp)
AAGCTGCCAGTTGAAGAACTGTATCTCGTA232880111.385031398532295No Hit
TAGCTTATCAGACTGATGTTGAATCTCGTA9299394.546281418515247No Hit
TAGCTTATCAGACTGATGTTGACATCTCGT7990403.9063430016919627No Hit
TATTGCACTTGTCCCGGCCTGTATCTCGTA4506512.2031404936617505No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTA3854021.8841514887090585No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGT3658391.7885119861283365No Hit
CACCCGTAGAACCGACCTTGCGATCTCGTA2759741.3491803959112658No Hit
TAATACTGCCGGGTAATGATGGAATCTCGT1977300.9666614959508308No Hit
TCCTTCATTCCACCGGAGTCTGATCTCGTA1742960.8520974667387144No Hit
TCCTTCATTCCACCGGAGTCTGTATCTCGT1505480.7359983558003625No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGT1439770.7038740818414645No Hit
TCAGTGCACTACAGAACTTTGTATCTCGTA1392790.6809065214916086No Hit
TTTGGCAATGGTAGAACTCACACTATCTCG1383490.6763599418565797No Hit
AACCCGTAGATCCGAACTTGTGAATCTCGT1240200.6063083939099886No Hit
GTTTGTGATGACTTACATGGAATCTCGTAT1054810.5156750177231052No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTA1008530.49304967304470315No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAA927620.4534944302199513Illumina Paired End PCR Primer 2 (100% over 23bp)
TGAGGTAGTAGATTGTATAGTTATCTCGTA910440.44509547988341397No Hit
AACCCGTAGATCCGAACTTGTATCTCGTAT908620.4442057191376341No Hit
TATGGCACTGGTAGAATTCACTGATCTCGT856650.4187986499298434No Hit
TGAGGTAGTAGGTTGTGTGGTTATCTCGTA823970.4028220668682578No Hit
ATGGCACTGGTAGAATTCACTGATCTCGTA728360.3560802949429764No Hit
CACCCGTAGAACCGACCTTGCATCTCGTAT690550.3375957598891651No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGT686820.3357722392398471No Hit
ATCACATTGCCAGGGATTTCCAATCTCGTA685030.3348971448799867No Hit
CAACACCAGTCGATGGGCTGTCATCTCGTA659930.32262626866071503No Hit
TGTAAACATCCTCGACTGGAAGATCTCGTA607210.29685253980493803No Hit
CTGTGCGTGTGACAGCGGCTGAATCTCGTA593000.2899055616744261No Hit
TTTGGCAATGGTAGAACTCACAATCTCGTA539940.2639656137782287No Hit
TAGCACCATCTGAAATCGGTTAATCTCGTA530150.2591794831731821No Hit
TTTGGCAATGGTAGAACTCACACTGATCTC512870.25073164488357996No Hit
CTTTCAGTCGGATGTTTGCAGCATCTCGTA480110.2347159514595425No Hit
TGTAAACATCCTCGACTGGAAGCATCTCGT472960.23122046281540734No Hit
TTTGGCAATGGTAGAACTCACACATCTCGT462870.22628766835116626No Hit
TGTAAACATCCTCGACTGGAAGCTATCTCG461990.2258574543641958No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTAT436360.21332747199368057No Hit
TAGCTTATCAGACTGATGTTGATCTCGTAT431410.2109075183169716No Hit
ACTCGGCGTGGCGTCGGTCGTGGATCTCGT426730.2086195621135377No Hit
TATGGCACTGGTAGAATTCACTATCTCGTA415780.20326633125293908No Hit
TTCACAGTGGCTAAGTTCCGCATCTCGTAT406940.19894463620200833No Hit
AAGCTGCCAGTTGAAGAACTGTAATCTCGT395660.1934300750962958No Hit
TAGCACCATCTGAAATCGGTTATCTCGTAT391810.19154788890329996No Hit
TGGCTCAGTTCAGCAGGAACAGATCTCGTA389350.19034524525790522No Hit
ATCACATTGCCAGGGATTTCCAAATCTCGT380020.18578399923695682No Hit
TCGTTTCCGGCTCGAAGGACCAATCTCGTA374520.18309516181839133No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTA367890.17985389053019327No Hit
AACCCGTAGATCCGAACTTGTGATCTCGTA361960.17695483491344902No Hit
ATCACATTGCCAGGGATTTCCATCTCGTAT357440.17474509943491884No Hit
TCCTTCATTCCACCGGAGTCTATCTCGTAT344670.16850210782854036No Hit
TAATACTGCCGGGTAATGATGGATCTCGTA341470.1669376933304659No Hit
ATCGCGTATGCCGTCTTCTGCTTGAAAAAA323230.1580205306914414Illumina Paired End PCR Primer 2 (95% over 24bp)
TAACACTGTCTGGTAAAGATGGCATCTCGT307760.1504575643523126No Hit
TAGCTTATCAGACTGATGTTGACTATCTCG293420.14344703188281635No Hit
TTATGGTTTGCCTGGGACTGAATCTCGTAT293180.14332970079546078No Hit
CACTAGATTGTGAGCTCCTGGAATCTCGTA281780.13775647414607045No Hit
CTTTCAGTCGGATGTTTACAGTATCTCGTA280770.13726270582011568No Hit
TCCTTCATTCCACCGGAGTCTGAATCTCGT276070.13496497202606883No Hit
TAGCACCATTTGAAATCAGTGTTATCTCGT273530.1337232180182222No Hit
TAGCTTATCAGACTGATGTTGACAATCTCG254090.12421939994241976No Hit
AGCTGCCAGTTGAAGAACTGTATCTCGTAT245640.12008836790844185No Hit
TGTAAACATCCTTGACTGGAAGCATCTCGT234720.11474980343376272No Hit
AGCAGCATTGTACAGGGCTATGATCTCGTA234280.11453469644027746No Hit
CAAAGTGCTTACAGTGCAGGTAGATCTCGT232520.1136742684663365No Hit
AACTCGTATGCCGTCTTCTGCTTGAAAAAA231570.11320983291222064Illumina Single End Adapter 1 (95% over 24bp)
CTAGACTGAAGCTCCTTGAGGATCTCGTAT229940.11241295927726397No Hit
CTCGGCGTGGCGTCGGTCGTGGATCTCGTA220650.10787126843754152No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTA215260.10523620776734732No Hit
TTTGGCAATGGTAGAACTCACACTGGATCT214880.10505043354570097No Hit
AACCCGTAGATCCGAACTTGTGTATCTCGT212660.1039651209876618No Hit
AAGCTGCCAGTTGAAGAACTGTTATCTCGT210230.1027771437281865No Hit
TGGCTCAGTTCAGCAGGAACAGTATCTCGT205380.10040607800454239No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)