FastQCFastQC Report
Wed 8 May 2024
EGAF00000828794

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000828794
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29344727
Total Bases880.3 Mbp
Sequences flagged as poor quality0
Sequence length30
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTATGCCGTCTTCTGCTTGAAAAAA467786215.941064982475387Illumina Paired End PCR Primer 2 (100% over 24bp)
AAGCTGCCAGTTGAAGAACTGTATCTCGTA397255013.537525838969296No Hit
TAGCTTATCAGACTGATGTTGAATCTCGTA15500215.282110820114292No Hit
TAGCTTATCAGACTGATGTTGACATCTCGT12330384.201906529919327No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTA6127562.0881298367505687No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGT5839531.9899759162864252No Hit
TATTGCACTTGTCCCGGCCTGTATCTCGTA5821031.983671546850649No Hit
CACCCGTAGAACCGACCTTGCGATCTCGTA3919461.335660747499883No Hit
TAATACTGCCGGGTAATGATGGAATCTCGT2969331.011878556580199No Hit
TCAGTGCACTACAGAACTTTGTATCTCGTA2146290.7314056798006674No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGT2084940.7104990276447282No Hit
TCCTTCATTCCACCGGAGTCTGATCTCGTA2041140.6955730070346199No Hit
TTTGGCAATGGTAGAACTCACACTATCTCG1849500.6302665552145024No Hit
TGAGGTAGTAGGTTGTGTGGTTATCTCGTA1595300.5436411113996733No Hit
AACCCGTAGATCCGAACTTGTGAATCTCGT1421390.48437663093611333No Hit
TCCTTCATTCCACCGGAGTCTGTATCTCGT1392030.4743714262531732No Hit
AACCCGTAGATCCGAACTTGTATCTCGTAT1342780.4575881724849578No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTA1325960.45185630794929527No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAA1244750.42418183000986853Illumina Paired End PCR Primer 2 (100% over 23bp)
TATGGCACTGGTAGAATTCACTGATCTCGT1219910.4157169361296154No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTA1194060.4069078577558415No Hit
ATCACATTGCCAGGGATTTCCAATCTCGTA1139880.3884445747271733No Hit
ATGGCACTGGTAGAATTCACTGATCTCGTA993230.33846966782141136No Hit
GTTTGTGATGACTTACATGGAATCTCGTAT895890.30529846128744015No Hit
CTGTGCGTGTGACAGCGGCTGAATCTCGTA883070.30092970365681027No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGT873040.2975117130924408No Hit
CACCCGTAGAACCGACCTTGCATCTCGTAT809080.27571563368096763No Hit
TAGCACCATCTGAAATCGGTTAATCTCGTA802230.2733813131060991No Hit
TTCACAGTGGCTAAGTTCCGCATCTCGTAT733610.24999721415026283No Hit
TTTGGCAATGGTAGAACTCACAATCTCGTA716580.24419378650208604No Hit
TGGCTCAGTTCAGCAGGAACAGATCTCGTA715240.2437371456889001No Hit
ATCACATTGCCAGGGATTTCCATCTCGTAT686830.23405567889590523No Hit
ATCACATTGCCAGGGATTTCCAAATCTCGT671180.2287225231299647No Hit
TGTAAACATCCTCGACTGGAAGCTATCTCG667310.22740371719934555No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTAT664270.2263677559515207No Hit
TTTGGCAATGGTAGAACTCACACTGATCTC649250.22124928952312284No Hit
CAACACCAGTCGATGGGCTGTCATCTCGTA644130.21950451268468096No Hit
TAGCTTATCAGACTGATGTTGATCTCGTAT629640.21456665792119997No Hit
TATGGCACTGGTAGAATTCACTATCTCGTA627390.21379991028711906No Hit
TGTAAACATCCTCGACTGGAAGCATCTCGT626460.21348298793169895No Hit
TTTGGCAATGGTAGAACTCACACATCTCGT624220.21271964806488064No Hit
TGTAAACATCCTCGACTGGAAGATCTCGTA612840.20884160892006254No Hit
TTATGGTTTGCCTGGGACTGAATCTCGTAT590930.20137519084774586No Hit
TAATACTGCCGGGTAATGATGGATCTCGTA589330.20082994808573273No Hit
TAGCACCATCTGAAATCGGTTATCTCGTAT575390.19607952052169372No Hit
AACCCGTAGATCCGAACTTGTGATCTCGTA570410.194382452424928No Hit
ACTCGGCGTGGCGTCGGTCGTGGATCTCGT549860.1873794907003224No Hit
AAGCTGCCAGTTGAAGAACTGTAATCTCGT535610.18252342235114336No Hit
CTTTCAGTCGGATGTTTGCAGCATCTCGTA504900.17205816908775468No Hit
TCGTTTCCGGCTCGAAGGACCAATCTCGTA482120.16429527526359333No Hit
CTAGACTGAAGCTCCTTGAGGATCTCGTAT433370.1476824098585071No Hit
AGCAGCATTGTACAGGGCTATGATCTCGTA430810.14681002143928618No Hit
AGCTGCCAGTTGAAGAACTGTATCTCGTAT401840.13693771967958673No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTA383430.13066402014917364No Hit
TAACACTGTCTGGTAAAGATGGCATCTCGT381550.13002335990380826No Hit
TAGCACCATTTGAAATCAGTGTTATCTCGT377860.1287658937839156No Hit
CTTTCAGTCGGATGTTTACAGTATCTCGTA375710.12803322382246052No Hit
CACTAGATTGTGAGCTCCTGGAATCTCGTA371870.12672464119362908No Hit
CAAAGTGCTTACAGTGCAGGTAGATCTCGT370180.12614872852625278No Hit
TGGCTCAGTTCAGCAGGAACAGTATCTCGT363840.12398820408177592No Hit
TAGCTTATCAGACTGATGTTGACTATCTCG356700.12155505825629251No Hit
ATCGCGTATGCCGTCTTCTGCTTGAAAAAA354070.1206588154662335Illumina Paired End PCR Primer 2 (95% over 24bp)
TGTAAACATCCCCGACTGGAAGATCTCGTA347500.11841991237471729No Hit
TCCTTCATTCCACCGGAGTCTGAATCTCGT336070.11452483439358628No Hit
TCCTTCATTCCACCGGAGTCTATCTCGTAT333420.1136217760690021No Hit
TAGCTTATCAGACTGATGTTGACAATCTCG328440.11192470797223637No Hit
TGAGGTAGTAGGTTGTGTGGATCTCGTATG320020.1090553679371425No Hit
AAGCTGCCAGTTGAAGAACTGTTATCTCGT315520.10752187266898071No Hit
AAGTTGCCAGTTGAAGAACTGTATCTCGTA298340.10166732851186519No Hit
TAAAGTGCTTATAGTGCAGGTAGATCTCGT293970.10017813421811694No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)