FastQCFastQC Report
Wed 8 May 2024
EGAF00000828795

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000828795
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26788094
Total Bases803.6 Mbp
Sequences flagged as poor quality0
Sequence length30
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTATGCCGTCTTCTGCTTGAAAAAA415060015.494196787572868Illumina Paired End PCR Primer 2 (100% over 24bp)
TAGCTTATCAGACTGATGTTGAATCTCGTA12754764.761354055275452No Hit
TACAGTACTGTGATAACTGAATCTCGTATG11620294.337856213286395No Hit
GTTTGTGATGACTTACATGGAATCTCGTAT4830061.803062211144996No Hit
TCAGTGCACTACAGAACTTTGATCTCGTAT4570851.7062990745067568No Hit
TAGCTTATCAGACTGATGTTGATCTCGTAT4270631.5942268979644465No Hit
TCTCCCAACCCTTGTACCAGTGATCTCGTA3949371.4743004858800333No Hit
TACAGTACTGTGATAACTGAATATCTCGTA3891571.4527237361493506No Hit
TCAGTGCACTACAGAACTTTGAATCTCGTA3561401.3294712195649305No Hit
GTAGTGTTTCCTACTTTATGGATCTCGTAT3426981.2792922109352014No Hit
TAGCACCATCTGAAATCGGTTATCTCGTAT2994051.1176793690510418No Hit
TATTGCACTTGTCCCGGCCTGTATCTCGTA2238720.8357145528905491No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGT2218360.8281141614629246No Hit
TCTCCCAACCCTTGTACCAGTATCTCGTAT2212860.8260610105369945No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGT2123600.7927402375099923No Hit
TCAGTGCACTACAGAACTTTGTATCTCGTA2051950.7659932804476496No Hit
TCTCCCAACCCTTGTACCAGATCTCGTATG1944970.7260576284374692No Hit
GTACAGTACTGTGATAACTGAATCTCGTAT1850650.6908479565586114No Hit
GCGGGTGATGCGAACTGGAGTCTGAGCATC1793800.6696258419878622No Hit
AAGCTGCCAGTTGAAGAACTGTATCTCGTA1492280.5570683752266958No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTA1439090.5372125392721109No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTA1426400.5324753601357379No Hit
TACAGTACTGTGATAACTGAAATCTCGTAT1349240.5036715191457817No Hit
CTTTCAGTCGGATGTTTACAGTATCTCGTA1286850.4803813216423684No Hit
TGTAGTGTTTCCTACTTTATGGATCTCGTA1177280.43947882219615925No Hit
TGTAAACATCCTTGACTGGATCTCGTATGC1157930.4322554639385691No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTA1118200.4174242482499875No Hit
TAGCTTATCAGACTGATGTTGACATCTCGT1031480.38505165765059657No Hit
GTAGTGTTTCCTACTTTATGGAATCTCGTA1029870.3844506443795516No Hit
TGAGGTAGTAGGTTGTATAGATCTCGTATG1003840.3747336409973774No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTAT952470.3555572113491912No Hit
CACTAGATTGTGAGCTCCTGGAATCTCGTA925960.34566102388620856No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAA903210.33716844505622534Illumina Paired End PCR Primer 2 (100% over 23bp)
TTAATGCTAATCGTGATAGGGGTATCTCGT846050.31583060743328734No Hit
TTAATGCTAATCGTGATAGGGGATCTCGTA776870.2900057017867714No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTA774890.2892665674534366No Hit
CTTTCAGTCGGATGTTTACAGATCTCGTAT753700.2813563368860808No Hit
TTCAAGTAATCCAGGATAGGATCTCGTATG748680.2794823700409592No Hit
TAGCACCATTTGAAATCGGTTATCTCGTAT714330.26665950925810544No Hit
TACAGTACTGTGATAACTGAAAATCTCGTA710410.2651961725981699No Hit
TTAATGCTAATCGTGATAGGGATCTCGTAT701880.26201192216213665No Hit
TCGTTTCCGGCTCGAAGGACCAATCTCGTA662360.24725909950890868No Hit
TCTCCCAACCCTTGTACCAGTGAATCTCGT649050.24229047426815808No Hit
TAGCACCATCTGAAATCGGTTAATCTCGTA645370.24091672964862673No Hit
TGAGGTAGTAGGTTGTATAGAATCTCGTAT622960.23255107287588284No Hit
TGTAAACATCCTTGACTGGAAGATCTCGTA561180.20948858847516363No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTA526340.19648281060981793No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGT522590.19508293497850202No Hit
ATGTGTGACTGAAAGGTATTTTCTGAGCAT502570.18760946560811678No Hit
TCTCCCAACCCTTGTACCAGTGTATCTCGT497170.18559364469902187No Hit
TTAATGCTAATCGTGATAGGGAATCTCGTA496640.1853957956097959No Hit
CTTTCAGTCGGATGTTTACAATCTCGTATG491380.18343223672427011No Hit
TGTAAACATCCTTGACTGGAAGCATCTCGT460670.1719681885542137No Hit
CTAGCACCATCTGAAATCGGTTATCTCGTA420840.15709964284879693No Hit
TAGCTTATCAGACTGATGTTGAAATCTCGT403460.15061168592285812No Hit
TGTTTGTGATGACTTACATGGAATCTCGTA390740.14586330778143455No Hit
TTAATGCTAATCGTGATAGGGGAATCTCGT381440.14239161621577107No Hit
TGAGGTAGTAGATTGTATAGTATCTCGTAT375590.14020781023091825No Hit
ATGCAGTGTGGAACACAATGAACTGAACAT368690.137632039069297No Hit
CGCGGGTGATGCGAACTGGAGTCTGAGCAT364110.13592232429824982No Hit
TGTCGCTGATGCCATCACCGCAGCGCTCTG362650.13537730605245749No Hit
ATCGCGTATGCCGTCTTCTGCTTGAAAAAA353610.13200267253056525Illumina Paired End PCR Primer 2 (95% over 24bp)
TAGCACCATCTGAAATCGGTATCTCGTATG347880.12986366256591456No Hit
TTAATGCTAATCGTGATAGGGGTTATCTCG346110.12920292126793342No Hit
TACAGTACTGTGATAACTGAAGATCTCGTA341780.12758653153897398No Hit
TATTGCACTTGTCCCGGCCTGTAAATCTCG333110.12435001907937161No Hit
TTCAAGTAATCCAGGATAGGCTATCTCGTA305660.11410292945813912No Hit
AATGTGTGACTGAAAGGTATTTTCTGAGCA303490.11329286809281765No Hit
CCTACTGTGGAATCTGAAGACAATCTCGTA297680.11112399411469887No Hit
TAGCAGCACGTAAATATTGGCGATCTCGTA291820.10893645512816254No Hit
TGAGGTAGTAGTTTGTACAGTTATCTCGTA279630.10438592607596495No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)