FastQCFastQC Report
Wed 8 May 2024
EGAF00000828796

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000828796
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28206076
Total Bases846.1 Mbp
Sequences flagged as poor quality0
Sequence length30
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTATGCCGTCTTCTGCTTGAAAAAA404899814.355055981555179Illumina Paired End PCR Primer 2 (100% over 24bp)
TAGCTTATCAGACTGATGTTGAATCTCGTA17025996.036284522526281No Hit
TCAGTGCACTACAGAACTTTGATCTCGTAT7781252.7587141153558545No Hit
GTTTGTGATGACTTACATGGAATCTCGTAT6684862.370007086416416No Hit
TACAGTACTGTGATAACTGAATCTCGTATG6543072.3197377756480555No Hit
TCAGTGCACTACAGAACTTTGAATCTCGTA5444661.9303145889559399No Hit
TCTCCCAACCCTTGTACCAGTGATCTCGTA4001811.4187758694261479No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTA3970681.4077392402970197No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGT3716441.317602632851163No Hit
TAGCACCATCTGAAATCGGTTATCTCGTAT3655631.2960434482272543No Hit
TAGCTTATCAGACTGATGTTGATCTCGTAT3616041.2820074653418647No Hit
GTAGTGTTTCCTACTTTATGGATCTCGTAT3496551.2396442525362266No Hit
TCAGTGCACTACAGAACTTTGTATCTCGTA3042121.07853357553174No Hit
TATTGCACTTGTCCCGGCCTGTATCTCGTA2810560.9964377887941591No Hit
TACAGTACTGTGATAACTGAATATCTCGTA2646270.9381914733548898No Hit
TTAATGCTAATCGTGATAGGGGTATCTCGT1977260.7010049891378014No Hit
TCTCCCAACCCTTGTACCAGTATCTCGTAT1858350.6588474057859023No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGT1785900.633161450745577No Hit
TTAATGCTAATCGTGATAGGGGATCTCGTA1705560.6046782260673197No Hit
TTAATGCTAATCGTGATAGGGATCTCGTAT1678160.5949640070458578No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTA1549670.5494099923718563No Hit
GTAGTGTTTCCTACTTTATGGAATCTCGTA1521130.5392916051137351No Hit
AAGCTGCCAGTTGAAGAACTGTATCTCGTA1515700.5373664879864891No Hit
GTACAGTACTGTGATAACTGAATCTCGTAT1356540.4809389296121871No Hit
CTTTCAGTCGGATGTTTACAGTATCTCGTA1337710.474263063036489No Hit
TCTCCCAACCCTTGTACCAGATCTCGTATG1305390.4628045389936551No Hit
TAGCTTATCAGACTGATGTTGACATCTCGT1246800.44203241883060945No Hit
TGTAGTGTTTCCTACTTTATGGATCTCGTA1231180.43649460492129427No Hit
GCGGGTGATGCGAACTGGAGTCTGAGCATC1181240.41878919988728674No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGT1165310.4131414805802835No Hit
TCGTTTCCGGCTCGAAGGACCAATCTCGTA1078980.3825345999918599No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTA996090.353147314784233No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTAT995320.3528743239577175No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTA986970.3499139688909581No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAA975780.3459467385679596Illumina Paired End PCR Primer 2 (100% over 23bp)
CTTTCAGTCGGATGTTTACAGATCTCGTAT959970.3403415632858679No Hit
TACAGTACTGTGATAACTGAAATCTCGTAT906170.32126765878387337No Hit
TGAGGTAGTAGGTTGTATAGATCTCGTATG877230.3110074581093804No Hit
TTAATGCTAATCGTGATAGGGAATCTCGTA846670.300172913098582No Hit
CACTAGATTGTGAGCTCCTGGAATCTCGTA738970.26198965074050007No Hit
TTCAAGTAATCCAGGATAGGATCTCGTATG709160.25142100588539856No Hit
TAGCACCATCTGAAATCGGTTAATCTCGTA691100.24501813013621607No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTA667600.23668659192437827No Hit
TTAATGCTAATCGTGATAGGGGAATCTCGT653560.23170894100973138No Hit
TTAATGCTAATCGTGATAGGATCTCGTATG618210.21917618033788183No Hit
TTAATGCTAATCGTGATAGGGGTTATCTCG608660.2157903850220073No Hit
TGTAAACATCCTTGACTGGATCTCGTATGC579670.20551245767046789No Hit
CTTTCAGTCGGATGTTTACAATCTCGTATG560500.19871604969085385No Hit
TCTCCCAACCCTTGTACCAGTGTATCTCGT535220.189753441776162No Hit
TGTAAACATCCTTGACTGGAAGATCTCGTA522850.18536786187486695No Hit
TTCAAGTAATCCAGGATAGGCTATCTCGTA485510.17212957945656815No Hit
TAGCACCATTTGAAATCGGTTATCTCGTAT484880.1719062233257827No Hit
TGAGGTAGTAGGTTGTATAGAATCTCGTAT470010.16663430957216452No Hit
TCTCCCAACCCTTGTACCAGTGAATCTCGT444560.1576114309555147No Hit
CATTGCACTTGTCTCGGTCTGTATCTCGTA444020.15741998284341288No Hit
TGTTTGTGATGACTTACATGGAATCTCGTA440010.15599830334428652No Hit
TGAGGTAGTAGATTGTATAGTATCTCGTAT439850.15594157797773786No Hit
AGCAGCATTGTACAGGGCTATGATCTCGTA432250.15324712306667543No Hit
TAGCAGCACGTAAATATTGGCGATCTCGTA431990.15315494434603383No Hit
ATGTGTGACTGAAAGGTATTTTCTGAGCAT428150.15179353554886543No Hit
TAGCTTATCAGACTGATGTTGAAATCTCGT427830.15168008481576806No Hit
TAGCACCATCTGAAATCGGTATCTCGTATG401320.14228140064573322No Hit
TGTAAACATCCTTGACTGGAAGCATCTCGT363010.128699220692733No Hit
CTAGCACCATCTGAAATCGGTTATCTCGTA353090.12518224796671468No Hit
TACAGTACTGTGATAACTGAAAATCTCGTA334600.11862692279493255No Hit
TTCAAGTAATTCAGGATAGGTTATCTCGTA334280.11851347206183518No Hit
ATCGCGTATGCCGTCTTCTGCTTGAAAAAA324950.11520567412496513Illumina Paired End PCR Primer 2 (95% over 24bp)
TGAGGTAGTAGTTTGTACAGTTATCTCGTA324510.11504967936695626No Hit
TCAGTGCACTCCAGAACTTTGATCTCGTAT305770.10840572080994179No Hit
TATTGCACTTGTCCCGGCCTGTAAATCTCG298050.10566872187396786No Hit
TACAGTACTGTGATAACTGAAGATCTCGTA293950.10421513435615788No Hit
ATGCAGTGTGGAACACAATGAACTGAACAT289280.10255946272001819No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)