FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000828797

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000828797
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37273829
Sequences flagged as poor quality0
Sequence length30
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTATGCCGTCTTCTGCTTGAAAAAA386738910.375615019320929Illumina Single End Adapter 1 (95% over 24bp)
TAGCTTATCAGACTGATGTTGAATCTCGTA33852659.082149837624677No Hit
TACAGTACTGTGATAACTGAATCTCGTATG15253794.092359279750949No Hit
TCAGTGCACTACAGAACTTTGAATCTCGTA8750282.3475667069245825No Hit
TAGCACCATCTGAAATCGGTTATCTCGTAT8567702.298583276754315No Hit
TCAGTGCACTACAGAACTTTGATCTCGTAT8013492.1498971838927523No Hit
TAGCTTATCAGACTGATGTTGATCTCGTAT6506311.7455437701342675No Hit
TACAGTACTGTGATAACTGAATATCTCGTA6419051.7221332426030072No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGT5728011.53673774701279No Hit
TATTGCACTTGTCCCGGCCTGTATCTCGTA5569831.4943004648113827No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTA4746131.2733143139117797No Hit
TCTCCCAACCCTTGTACCAGTGATCTCGTA3981321.068127452105873No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGT3182390.8537867145336745No Hit
TCAGTGCACTACAGAACTTTGTATCTCGTA2783850.7468645091439358No Hit
TAGCTTATCAGACTGATGTTGACATCTCGT2773170.7439992279837953No Hit
CTTTCAGTCGGATGTTTACAGTATCTCGTA2717980.729192592475541No Hit
TGAGGTAGTAGGTTGTATAGATCTCGTATG2569260.6892932840358311No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTA2530730.6789562725095938No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTA2444690.6558730523767762No Hit
GCGGGTGATGCGAACTGGAGTCTGAGCATC2372710.6365619158686381No Hit
TTAATGCTAATCGTGATAGGGATCTCGTAT2347750.6298655284381972No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTAT2291200.6146940256661048No Hit
TCTCCCAACCCTTGTACCAGATCTCGTATG2249590.6035306971011751No Hit
GTACAGTACTGTGATAACTGAATCTCGTAT2215010.5942534103485853No Hit
TCTCCCAACCCTTGTACCAGTATCTCGTAT2210370.5930085690954906No Hit
AAGCTGCCAGTTGAAGAACTGTATCTCGTA2163380.5804018685603779No Hit
TACAGTACTGTGATAACTGAAATCTCGTAT2076480.5570879235401333No Hit
TTAATGCTAATCGTGATAGGGGTATCTCGT1964980.5271741736004637No Hit
TTAATGCTAATCGTGATAGGGGATCTCGTA1955020.5245020574623551No Hit
TTCAAGTAATCCAGGATAGGATCTCGTATG1825300.4897001593262662No Hit
TGAGGTAGTAGGTTGTATAGAATCTCGTAT1728230.46365775836982026No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTA1683780.4517325011068758No Hit
CACTAGATTGTGAGCTCCTGGAATCTCGTA1455870.3905877230911801No Hit
TTAATGCTAATCGTGATAGGATCTCGTATG1436770.3854634843122771No Hit
TTAATGCTAATCGTGATAGGGAATCTCGTA1432810.3844010766911014No Hit
CTTTCAGTCGGATGTTTACAGATCTCGTAT1425170.3823513811795402No Hit
TGTAAACATCCTTGACTGGATCTCGTATGC1397690.3749789161719876No Hit
GTTTGTGATGACTTACATGGAATCTCGTAT1301050.3490518776592552No Hit
TAGCACCATCTGAAATCGGTATCTCGTATG1238490.33226798352270165No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTA1234980.33132630404029595No Hit
TAGCACCATCTGAAATCGGTTAATCTCGTA1202530.32262046381121723No Hit
AACTTGACCGCTCTGACCAATCTCGTATGC1175790.315446529520753No Hit
GTAGTGTTTCCTACTTTATGGATCTCGTAT1155810.3100862001593665No Hit
TAGCACCATTTGAAATCGGTTATCTCGTAT1058420.2839579480820175No Hit
ATGTGTGACTGAAAGGTATTTTCTGAGCAT1056270.28338113586344993No Hit
CTTTCAGTCGGATGTTTACAATCTCGTATG1033630.2773071690595565No Hit
TGAGGTAGTAGATTGTATAGTATCTCGTAT1016780.2727865709745033No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGT944380.2533627548701798No Hit
TACAGTACTGTGATAACTGAAGATCTCGTA928430.2490836130626666No Hit
TTAATGCTAATCGTGATAGGGGAATCTCGT851090.22833447027940165No Hit
TACAGTACTGTGATAACTGAAAATCTCGTA791220.2122722621279397No Hit
AGCAGCATTGTACAGGGCTATGATCTCGTA789650.21185105506600893No Hit
TTTCTGTGTGGAATTTGAATATCTGAAAAT777450.2085779810815787No Hit
AATGTGTGACTGAAAGGTATTTTCTGAGCA764660.20514661909298346No Hit
TCGTTTCCGGCTCGAAGGACCAATCTCGTA733800.19686735162089194No Hit
TAGCACCATTTGAAATCGGTATCTCGTATG699100.18755787069796345No Hit
TGAGGTAGTAGGTTGTGTGGATCTCGTATG690850.18534452148718072No Hit
TAGCTTATCAGACTGATGTTGAAATCTCGT686900.18428479671353323No Hit
TTAATGCTAATCGTGATAGGGGTTATCTCG642230.1723005168049679No Hit
TGTAAACATCCTTGACTGGAAGATCTCGTA627350.16830843968297435No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAA605900.16255373173493928Illumina Single End Adapter 1 (95% over 23bp)
TGAGGTAGTAGATTGTATAGATCTCGTATG587140.15752070977199578No Hit
TGTAAACATCCCCGACTGGAAGAATCTCGT582760.15634562255463477No Hit
CATTGCACTTGTCTCGGTCTGTATCTCGTA580620.1557714931835954No Hit
ATGCAGTGTGGAACACAATGAACTGAACAT531760.14266310016070524No Hit
CTAGCACCATCTGAAATCGGTTATCTCGTA514540.13804323671710786No Hit
TATTGCACTTGTCCCGGCCTGTAAATCTCG507780.13622963178803016No Hit
CGCGGGTGATGCGAACTGGAGTCTGAGCAT502960.13493649927942739No Hit
TTCAAGTAATCCAGGATAGGCTATCTCGTA493290.1323421857196372No Hit
TAGCACCATCTGAAATCGGTAATCTCGTAT493160.13230730870177035No Hit
TGTAAACATCCTACACTCAGCTATCTCGTA488370.13102222473575226No Hit
TCTCCCAACCCTTGTACCAGTGAATCTCGT472520.12676991140352123No Hit
CACTAGATTGTGAGCTCCTGGAAATCTCGT471550.12650967519328374No Hit
TATTGCACTTGTCCCGGCCTGTTATCTCGT448900.1204330255418621No Hit
TAGCAGCACGTAAATATTGGCGATCTCGTA427570.11471051176416569No Hit
TGAGGTAGTAGGTTGTGTGGTTATCTCGTA421400.11305519483925304No Hit
TAGCACCATCTGAAATCGGATCTCGTATGC408200.10951383610200069No Hit
TAGCACCATTTGAAATCGGATCTCGTATGC395490.10610393689363118No Hit
TAGCACCATCTGAAATCGGTTAAATCTCGT375200.1006604392588698No Hit
AAAAGCTGGGTTGAGAGGGCGAATCTCGTA373970.10033044901289856No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)