FastQCFastQC Report
Mon 12 Sep 2022
EGAF00000828798

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000828798
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11316940
Sequences flagged as poor quality0
Sequence length30
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCTTATCAGACTGATGTTGAATCTCGTA7113406.28562137821708No Hit
ATCTCGTATGCCGTCTTCTGCTTGAAAAAA6255495.527545431892367Illumina Single End Adapter 1 (95% over 24bp)
TGTAAACATCCTCGACTGGAAGATCTCGTA4953824.377349354154038No Hit
TCAGTGCACTACAGAACTTTGAATCTCGTA3420353.0223275903203515No Hit
TACAGTACTGTGATAACTGAATCTCGTATG3068452.711377810609582No Hit
TCAGTGCACTACAGAACTTTGTATCTCGTA2602402.299561542254355No Hit
TCAGTGCACTACAGAACTTTGATCTCGTAT2570572.271435564737464No Hit
TGAGGTAGTAGGTTGTATAGATCTCGTATG1818451.6068389511652441No Hit
TATTGCACTTGTCCCGGCCTGTATCTCGTA1445721.2774831359006942No Hit
TAGCTTATCAGACTGATGTTGATCTCGTAT1420121.2548621800592739No Hit
TAGCTTATCAGACTGATGTTGACATCTCGT1265311.1180672513948118No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTAT1260171.1135253876047766No Hit
TGAGGTAGTAGGTTGTGTGGATCTCGTATG1087290.9607632451881868No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTA1049240.9271410823067013No Hit
TGAGGTAGTAGGTTGTATAGAATCTCGTAT1000860.8843910102907677No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGT931650.8232349027210536No Hit
AAGCTGCCAGTTGAAGAACTGTATCTCGTA903990.7987936668392693No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTA891060.787368316877177No Hit
TAATACTGCCGGGTAATGATGGAATCTCGT882420.7797337442806979No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTA793430.7010994137991365No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGT791440.6993409879349011No Hit
TAATACTGCCGGGTAATGATGGATCTCGTA714450.6313102305040055No Hit
TAGCACCATCTGAAATCGGTTATCTCGTAT703850.6219437409759175No Hit
TGTAAACATCCTTGACTGGATCTCGTATGC649780.5741658080717933No Hit
TACAGTACTGTGATAACTGAATATCTCGTA514070.4542482331796404No Hit
TCGTTTCCGGCTCGAAGGACCAATCTCGTA505870.4470024582616856No Hit
TGTAAACATCCTCGACTGGAAGCATCTCGT496810.438996760608433No Hit
GGGCCAGTGGCGCAATGGAATCTCGTATGC492400.4350999475123134No Hit
CTTTCAGTCGGATGTTTGCAGATCTCGTAT483750.4274565386049586No Hit
CTTTCAGTCGGATGTTTGCAGCATCTCGTA445950.39405528349536184No Hit
GTACAGTACTGTGATAACTGAATCTCGTAT425200.3757199384285858No Hit
TGAGATGAAGCACTGTAGCTATCTCGTATG409890.3621915464781116No Hit
CACCCGTAGAACCGACCTTGCATCTCGTAT397350.35111081264016597No Hit
TTTGGCAATGGTAGAACTCACAATCTCGTA388260.34307860605428675No Hit
TTCAAGTAATCCAGGATAGGATCTCGTATG379740.33555006918831415No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTA353410.31228406265297864No Hit
CACCCGTAGAACCGACCTTGCGATCTCGTA337690.29839338195660664No Hit
TGAGGTAGTAGATTGTATAGTATCTCGTAT311460.2752157385300267No Hit
TGAGGTAGTAGGTTGTGTGAATCTCGTATG303320.2680229814773252No Hit
TGTAAACATCCTCGACTGGAAATCTCGTAT291790.2578347150378106No Hit
TGAGGTAGTAGGTTGTGTGGTATCTCGTAT272450.24074528980448776No Hit
TACAGTACTGTGATAACTGAAATCTCGTAT261210.23081327638036434No Hit
TACCCTGTAGATCCGAATTTGTATCTCGTA258930.22879859750073783No Hit
GGCCGCGTGGCCTAATGGAATCTCGTATGC257630.22764987708691573No Hit
TTTGTTCGTTCGGCTCGCGTGAATCTCGTA242860.2145986459237214No Hit
TTTGGCAATGGTAGAACTCAATCTCGTATG223110.19714693194450092No Hit
TGAGATGAAGCACTGTAGCTCTATCTCGTA222590.19668744377897207No Hit
TAATACTGCCGGGTAATGATGATCTCGTAT211200.18662288569171523No Hit
TACAGTACTGTGATAACTGAAGATCTCGTA210370.1858894718890442No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTA191810.1694892789040147No Hit
GTTTGTGATGACTTACATGGAATCTCGTAT188960.1669709303044816No Hit
CTTTCAGTCGGATGTTTACAGATCTCGTAT188310.16639657009757056No Hit
TGAGGTAGTAGATTGTATAGATCTCGTATG186040.16439072752881964No Hit
TGTCAACATCCTCGACTGGAAGATCTCGTA182400.1611743103701177No Hit
TGAGGTAGTAGGTTGTGTGGAATCTCGTAT169550.14981965089502994No Hit
CTTTCAGTCGGATGTTTACAGTATCTCGTA169380.1496694336101455No Hit
TGAGGTAGTAGGTTGTGTGGTTATCTCGTA169330.14962525205576774No Hit
TGTAAACATCCTTGACTGGAAGATCTCGTA162110.14324543560361722No Hit
TGAGGTAGTAGGTTGTATGGATCTCGTATG161000.14226460509643066No Hit
GCGGGTGATGCGAACTGGAGTCTGAGCATC156700.13846499141994215No Hit
GGCTCCGTGGCGCAATGGAATCTCGTATGC156670.1384384824873155No Hit
TATTGCACTTGTCCCGGCCTGTTATCTCGT156520.13830593782418216No Hit
TACCGTACTGTGATAACTGAATCTCGTATG148530.13124572543461396No Hit
TCCTTCATTCCACCGGAGTCTATCTCGTAT148310.13105132659535174No Hit
TTCTCGTATGCCGTCTTCTGCTTGAAAAAA143420.1267303705772055Illumina Paired End PCR Primer 2 (95% over 24bp)
GTGAAATGTTTAGGACCACTAGATCTCGTA142980.1263415728986811No Hit
AGCAGCATTGTACAGGGCTATGATCTCGTA136050.1202180094619217No Hit
CATTGCACTTGTCTCGGTCTGTATCTCGTA131000.11575567246976658No Hit
TTTCAGTCGGATGTTTGCAGCATCTCGTAT130890.11565847305013545No Hit
CTTTCAGTCGGATGTTTGCAATCTCGTATG128200.11328150542461125No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGT127760.11289270774608685No Hit
TACCCTGTAGAACCGAATTTGTATCTCGTA125320.1107366478924515No Hit
TGTAAACATCCTCGACTGGAAGAATCTCGT123480.10911076669134943No Hit
CAACACCAGTCGATGGGCTGTCATCTCGTA120030.10606223943928307No Hit
CACTAGATTGTGAGCTCCTGGAATCTCGTA116650.10307556636334557No Hit
TAGCACCATTTGAAATCGGTTATCTCGTAT115470.10203288168003011No Hit
TCCTTCATTCCACCGGAGTCTGATCTCGTA114810.10144968516224351No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)