FastQCFastQC Report
Thu 9 May 2024
EGAF00000828799

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000828799
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14554898
Total Bases436.6 Mbp
Sequences flagged as poor quality0
Sequence length30
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCTTATCAGACTGATGTTGAATCTCGTA11063607.60128995751121No Hit
ATCTCGTATGCCGTCTTCTGCTTGAAAAAA8893606.11038291027529Illumina Paired End PCR Primer 2 (100% over 24bp)
TGTAAACATCCTCGACTGGAAGATCTCGTA7437545.109991152119376No Hit
TAGCTTATCAGACTGATGTTGACATCTCGT2895591.9894265147031605No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTA2715911.8659766629762709No Hit
TCAGTGCACTACAGAACTTTGTATCTCGTA2594551.7825957969612702No Hit
GGGCCAGTGGCGCAATGGAATCTCGTATGC2392661.6438864772532245No Hit
TAGCTTATCAGACTGATGTTGATCTCGTAT2286451.5709144784113223No Hit
AAGCTGCCAGTTGAAGAACTGTATCTCGTA2245031.5424567042654644No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTAT2095111.4394535777578104No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGT2070341.4224352516932788No Hit
TGAGGTAGTAGGTTGTATAGATCTCGTATG1687601.1594722271499258No Hit
TGAGGTAGTAGGTTGTGTGGATCTCGTATG1580841.0861223486416738No Hit
TTTGGCAATGGTAGAACTCACAATCTCGTA1521371.0452632509001436No Hit
TGTAAACATCCTCGACTGGAAGCATCTCGT1504491.033665780412889No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTA1351000.9282098713436535No Hit
TCAGTGCACTACAGAACTTTGATCTCGTAT1161130.7977589399802045No Hit
TTTGTTCGTTCGGCTCGCGTGAATCTCGTA1158760.796130622145205No Hit
TACAGTACTGTGATAACTGAATCTCGTATG1030970.7083319993036021No Hit
TAATACTGCCGGGTAATGATGGAATCTCGT989290.6796955911336513No Hit
CTTTCAGTCGGATGTTTGCAGCATCTCGTA857900.5894235741123023No Hit
GGCTCCGTGGCGCAATGGAATCTCGTATGC847420.5822232488334854No Hit
CTTTCAGTCGGATGTTTGCAGATCTCGTAT818780.5625460240257266No Hit
CACCCGTAGAACCGACCTTGCGATCTCGTA813140.5586710398107908No Hit
TCAGTGCACTACAGAACTTTGAATCTCGTA792320.5443665768045918No Hit
TGAGGTAGTAGGTTGTGTGGTATCTCGTAT751050.5160118607495566No Hit
GGCCGCGTGGCCTAATGGAATCTCGTATGC731830.502806684045467No Hit
TATTGCACTTGTCCCGGCCTGTATCTCGTA720560.4950635861549837No Hit
CACCCGTAGAACCGACCTTGCATCTCGTAT699470.48057361858530373No Hit
TGAGGTAGTAGGTTGTGTGGTTATCTCGTA693970.47679482192180256No Hit
TAATACTGCCGGGTAATGATGGATCTCGTA663650.45596334649682874No Hit
TTTGGCAATGGTAGAACTCAATCTCGTATG649750.4464132967472531No Hit
TACCCTGTAGATCCGAATTTGTATCTCGTA604400.4152554006218388No Hit
TAGCACCATCTGAAATCGGTTATCTCGTAT563860.38740223394214096No Hit
TGAGGTAGTAGGTTGTATAGAATCTCGTAT500090.34358880426369187No Hit
TGAGATGAAGCACTGTAGCTATCTCGTATG472130.32437877613432947No Hit
GACCCAGTGGCCTAATGGAATCTCGTATGC464890.3194045056172843No Hit
GTACAGTACTGTGATAACTGAATCTCGTAT434500.2985249364165932No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTA417170.2866182916568704No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTA407690.280105020316872No Hit
TCGTTTCCGGCTCGAAGGACCAATCTCGTA406720.2794385779962182No Hit
TTTGGCAATGGTAGAACTCACATCTCGTAT374130.2570474901301266No Hit
TGTAAACATCCTCGACTGGAAATCTCGTAT369830.25409315819320755No Hit
CAACACCAGTCGATGGGCTGTCATCTCGTA354740.24372551425643793No Hit
GCCCCAGTGGCCTAATGGAATCTCGTATGC345480.2373633947829796No Hit
TGAGATGAAGCACTGTAGCTCTATCTCGTA321800.22109395751175998No Hit
GGCCGCGTTGCCTAATGGAATCTCGTATGC319800.21971984963412317No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGT308720.21210729199201533No Hit
GGGCCAGTTGCGCAATGGAATCTCGTATGC307950.21157826045912517No Hit
AGCAGCATTGTACAGGGCTATGATCTCGTA303370.2084315534193369No Hit
TGTCAACATCCTCGACTGGAAGATCTCGTA301210.20694751691148916No Hit
TTTGGCAATGGTAGAACTCACACTATCTCG278610.19142009789419343No Hit
TACAGTACTGTGATAACTGAATATCTCGTA276500.1899704140832866No Hit
TATGGCACTGGTAGAATTCACTATCTCGTA276060.1896681103502065No Hit
TTTCAGTCGGATGTTTGCAGCATCTCGTAT256060.17592703157383857No Hit
TGAGGTAGTAGGTTGTGTGAATCTCGTATG252840.17371471789084336No Hit
TGTAAACATCCTTGACTGGATCTCGTATGC246620.1694412423913929No Hit
TGGTTAGTACTCTGCGTTGATCTCGTATGC240820.16545632954624623No Hit
GGCTCTGTGGCGCAATGGAATCTCGTATGC238770.1640478689716685No Hit
TTCTCGTATGCCGTCTTCTGCTTGAAAAAA224590.15430544411922364TruSeq Adapter, Index 20 (100% over 24bp)
TGAGGTAGTAGATTGTATAGTATCTCGTAT223700.15369396611367528No Hit
TTCAAGTAATCCAGGATAGGATCTCGTATG221120.1519213669515238No Hit
CACTAGATTGTGAGCTCCTGGAATCTCGTA196420.13495113466270942No Hit
GCGGGTGATGCGAACTGGAGTCTGAGCATC191450.131536476586782No Hit
CGCGACCTCAGATCAGACGTGGATCTCGTA180810.12422622267775425No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTA178370.12254981106703737No Hit
TTTCAGTCGGATGTTTGCAGATCTCGTATG175610.12065354219589859No Hit
TACCCTGTAGAACCGAATTTGTATCTCGTA175070.12028253306893666No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGT174670.1200077114934093No Hit
TAGCACCATTTGAAATCGGTTATCTCGTAT174240.1197122782997174No Hit
TGAGGTAGTAGGTTGTGTGGAATCTCGTAT173350.11910080029416902No Hit
TGTAAACATCCTCGACTGGAAGCTATCTCG171860.1180770899253296No Hit
CTTTCAGTCGGATGTTTGCAATCTCGTATG166710.11453876214041488No Hit
TTTCAGTCGGATGTTTGCAGTATCTCGTAT163290.11218903766965595No Hit
TACCCTGTAGATCCGAATTTGATCTCGTAT159080.1092965405872305No Hit
GGCTCCGTTGCGCAATGGAATCTCGTATGC155870.10709109744362344No Hit
GTGAAATGTTTAGGACCACTAGATCTCGTA151010.10375201530096603No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAA149030.10239164850210562Illumina Paired End PCR Primer 2 (100% over 23bp)
TAATACTGCCGGGTAATGATGATCTCGTAT147360.10124426842427889No Hit
CTTTCAGTCGGATGTTTGCAGTATCTCGTA145890.10023429913421586No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)