FastQCFastQC Report
Wed 8 May 2024
EGAF00000828800

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000828800
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34079860
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length30
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGATGAAGCACTGTAGCTATCTCGTATG28926558.487872309334604No Hit
ATCTCGTATGCCGTCTTCTGCTTGAAAAAA19265575.653066063064813Illumina Paired End PCR Primer 2 (100% over 24bp)
TGAGATGAAGCACTGTAGCTCTATCTCGTA18312205.373320195564184No Hit
TACCCTGTAGAACCGAATTTGTATCTCGTA17678365.187333516041439No Hit
TAGCTTATCAGACTGATGTTGAATCTCGTA9655982.8333391040925635No Hit
AAGCTGCCAGTTGAAGAACTGTATCTCGTA8812962.58597306444334No Hit
TGAGATGAAGCACTGTAGCTCATCTCGTAT5216761.5307457248944099No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTA4790991.4058126999348002No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTAT3292570.9661336636946278No Hit
TACAGTACTGTGATAACTGAATCTCGTATG3207920.9412949466341705No Hit
AACCCGTAGATCCGAACTTGTATCTCGTAT3095430.9082871819309116No Hit
TCAGTGCACTACAGAACTTTGTATCTCGTA3047030.8940852456553519No Hit
TAGCTTATCAGACTGATGTTGACATCTCGT2774450.814102522721631No Hit
TGTAAACATCCTCGACTGGAAGATCTCGTA2596760.7619632240273287No Hit
TACCCTGTAGATCCGAATTTGTATCTCGTA2568250.7535975793327789No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGT2504430.7348709765826503No Hit
TGAGGTAGTAGGTTGTATAGATCTCGTATG2177320.6388876010640889No Hit
TGAGGTAGTAGGTTGTGTGGATCTCGTATG2040070.5986145482992008No Hit
CACCCGTAGAACCGACCTTGCATCTCGTAT2019270.5925112368419354No Hit
TGAGGTAGTAGGTTGTATGGTTATCTCGTA1914670.5618186224943412No Hit
CACCCGTAGAACCGACCTTGCGATCTCGTA1877500.5509118875488338No Hit
TGAGATGAAGCACTGTAGCTAATCTCGTAT1777470.5215602411512253No Hit
AACCCGTAGATCCGATCTTGTATCTCGTAT1577670.4629332397492243No Hit
GTACAGTACTGTGATAACTGAATCTCGTAT1572760.4614925061311872No Hit
AACCCGTAGATCCGAACTTGTGAATCTCGT1463580.4294559895492528No Hit
TACCCTGTAGAACCGAATTTGATCTCGTAT1429660.4195028970189431No Hit
ACCCTGTAGAACCGAATTTGTATCTCGTAT1427040.4187341145180761No Hit
TAGCTTATCAGACTGATGTTGATCTCGTAT1373170.40292712470062964No Hit
TGAGATGAAGCACTGTAGCATCTCGTATGC1213220.3559932464511298No Hit
TGAGATGAAGCACTGTAGCTCAATCTCGTA1177010.34536820280365005No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTA1163560.34142159034690867No Hit
TAGCACCATCTGAAATCGGTTATCTCGTAT1095710.32151247100193486No Hit
TACAGTACTGTGATAACTGAATATCTCGTA1066410.31291501784338316No Hit
TGAGATGAAGCACTGTAGATCTCGTATGCC1041950.3057377583123874No Hit
TGGTAGACTATGGAACGTAGGATCTCGTAT952520.27949645333050077No Hit
TGAGATGAAGCACTGTAGCAATCTCGTATG896630.2630967380734545No Hit
TGAGGTAGTAGGTTGTATGGATCTCGTATG895350.26272114967608434No Hit
TGAGGTAGTAGGTTGTGTGGTATCTCGTAT873980.2564505840106151No Hit
TGAGGTAGTAGGTTGTGTGGTTATCTCGTA862860.2531876598084617No Hit
ACAGTAGTCTGCACATTGGTTATCTCGTAT769610.2258254582031734No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTA728700.21382130090909995No Hit
TCAGTGCACTACAGAACTTTGATCTCGTAT725830.21297916129937156No Hit
TGAGGTAGTAGGTTGTATAGAATCTCGTAT704380.2066851213590666No Hit
TGAGGTAGTAGGTTGTATGGTATCTCGTAT704220.20663817280939537No Hit
ACCCTGTAGAACCGAATTTGTGATCTCGTA685400.20111584965431195No Hit
TACCCTGTAGAACCGAATTTGTGATCTCGT680640.19971913030159164No Hit
TGTAAACATCCTCGACTGGAAGCATCTCGT672650.19737463710238246No Hit
TGAGATGAAGCACTGTAGCTCTAATCTCGT671960.19717217148192512No Hit
TGAGATGAAGCACTGTAGAAAATCTCGTAT619970.1819168271231161No Hit
ACCCTGTAGATCCGAATTTGTATCTCGTAT585050.17167030615736098No Hit
TGAGGTAGTAGATTGTATAGTATCTCGTAT579160.16994201267258727No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTA574420.16855116188857583No Hit
TCCCTGAGACCCTAACTTGTGAATCTCGTA561690.16481581790535524No Hit
GGGCCAGTGGCGCAATGGAATCTCGTATGC549580.16126239955211083No Hit
TTCAAGTAATCCAGGATAGGATCTCGTATG539500.1583046409228207No Hit
AACCCGTAGATCCGATCTTGTGAATCTCGT533880.15665557311561726No Hit
TGAGATGAAGCACTGTAGCTCTTATCTCGT527720.15484805395327328No Hit
TTCTCGTATGCCGTCTTCTGCTTGAAAAAA525960.1543316199068893TruSeq Adapter, Index 20 (100% over 24bp)
TGGTAGACTATGGAACGTAGATCTCGTATG521610.15305520621270158No Hit
TGAGATGAAGCACTGTAGAATCTCGTATGC516100.15143841553339715No Hit
TATTGCACTTGTCCCGGCCTGTATCTCGTA483080.14174940859498836No Hit
TCGGATCCGTCTGAGCTTGGCATCTCGTAT435430.12776754364601264No Hit
TCAGTGCACTACAGAACTTTGAATCTCGTA427370.1254025104563223No Hit
TACCCTGTAGATCCGAATTTGATCTCGTAT419850.1231959286217725No Hit
TACAGTACTGTGATAACTGAAATCTCGTAT416550.12222761478480253No Hit
TGAGATGAAGCACTGTAGCAAATCTCGTAT403590.11842478226142948No Hit
CTTTCAGTCGGATGTTTGCAGCATCTCGTA400640.1175591683768654No Hit
TACAGTACTGTGATAACTGAAGATCTCGTA392900.1152880322865176No Hit
AAGCTGCCAGTTGAAGACCTGTATCTCGTA387850.1138062186875181No Hit
CTTTCAGTCGGATGTTTGCAGATCTCGTAT387100.11358614736093399No Hit
TACACTGTAGAACCGAATTTGTATCTCGTA377350.11072522011534086No Hit
AACCCGTAGATCCGAACTTGTGATCTCGTA362870.10647637637009072No Hit
AGCAGCATTGTACAGGGCTATGATCTCGTA358840.10529385977524557No Hit
CGCGACCTCAGATCAGACGTGGATCTCGTA346970.10181086424650805No Hit
TGAGGTAGTAGGTTGTGTGAATCTCGTATG343100.10067529620133417No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)